Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 928 results for: Por

Showing: 701 - 720 of 928 results

  1. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...all of which need to be represented in the same proportion after amplification as they were prior to amplification...this guide, we hope you feel more confident incorporating them into your research. And, of course, if ...

  2. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...sharing their ideas? And how do we give people the opportunity to speak out and prevent groupthink? Here are...implemented, impacted the entire company. It is important to note that the person who submitted the idea...

  3. The Challenges of Cell Culture

    Type
    Blog Post
    ...been known about since 1966 when Stanley Gartler reported that most of the 18 cell lines he had studied ...repository like the ATCC, there are times when it is important to check their identity. For example, perhaps ...

  4. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...single-use aliquots, and stored at -80°C. Some reports suggest rapidly freezing virus in a dry ice/ethanol...protein attaches to cell surface receptors, other important binding events take place during the course of...

  6. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...added to the growth medium. Induction timing is important, as you typically want to make sure your cells... optimal because of the high cell-density they support; however, minimal media supplemented with M9 salts...

  7. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ... theory, is an easy technique, but it is very important to follow some simple rules to avoid common pitfalls...purpose of your studies. These parameters are less important when designing a biosensor, as the distance between...

  8. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ... to therapeutic uses, and in those cases it's important to identify the right tissue-specific promoters...Posts Learn about Mammalian Vectors Read about Reporter Gene like Luciferase and GFP Resources on Addgene.org...

  9. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...option for the experiment above, but it’s also important not to load yourself up with too many tasks. If...ll discuss in the planning section below, it’s important to dedicate specific amounts of time to specific...

  10. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...merely co-opted toward that aim (i.e. sacB). Importantly, to prevent activation of toxins before the second...genome editing in Escherichia coli." Scientific reports 5 (2015): 15096. PubMed PMID: 28060411. Thomason...

  11. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ... areas of microbial research like metabolism. Transport, biofilms, and much more. Because of it’s BSL-...designed for use in Arabidopsis. There are also important tips for plant transformations and the use of ...

  12. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ... out of fashion but is still useful and most importantly, effective. In the research world, there is a...plasmids The design of shRNA plasmids requires three important steps: Designing the shRNA sequence Choosing ...

  13. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...purification and minimizes sample loss while also supporting imaging, making it easy to identify dozens or...Integrase Gene Editing (PASSIGE), their previously-reported technique for precisely integrating large DNA ...

  14. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...cells to make CAR T cells. The extracellular scFv portion of the receptors recognizes an antigen of interest...interest while the intracellular T cell receptor portion of the CAR facilitates signal transduction and release...

  16. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...better to reduce off-target effects, but it’s important to not let ‘perfection’ get in the way of ‘good... score their probability of off-target events: CRISPOR, Cas-OFFinder, CCTop, and many more. While such...

  17. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...protocol is a good place to get started.  One important consideration when deciding whether you can do...specific considerations to keep in mind. Most importantly, make sure you are using primary antibodies from...

  18. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...some DNA during the gel purification step, it is important to digest plenty of starting material. We recommend...be cut with both enzymes, and therefore it is important that the digest go at least 4 hours and as long...running a gel for purification purposes it is important to have nice crisp bands and to have space to ... your favorite gel purification method, it is important to determine the concentration of recovered DNA...ratios of recipient plasmid to insert. It is also important to set up negative controls in parallel. For instance...

  20. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...a pressurized buffer called sheath fluid and transported through tubes or capillaries to a laser. As the...tag your protein of interest with a fluorescent reporter on the N- or C-terminus of the protein. One downside...
Showing: 701 - 720 of 928 results