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We narrowed to 921 results for: Por

Showing: 701 - 720 of 921 results

  1. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ... to therapeutic uses, and in those cases it's important to identify the right tissue-specific promoters...Posts Learn about Mammalian Vectors Read about Reporter Gene like Luciferase and GFP Resources on Addgene.org...

  2. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...500ML Benzamide, Millipore Sigma 12072 Antipain, Millipore Sigma 10791 Leupeptin, Millipore Sigma L2884 Aprotinin...that sharing the full details of our protocols supports reproducibility and accelerates science. Here,...information is solely for transparency intended to support reproducibility in science. General Considerations... Aprotinin saline solution, Millipore Sigma A6279 Before Starting Warm the DNA and working stock of PEI-MAX...

  3. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...IgG standards in Google Sheets. Open exported CSV file. Select import. Choose file. Format as needed. Select...that sharing the full details of our protocols supports reproducibility and accelerates science. Here,...information is solely for transparency intended to support reproducibility in science. Last Update: June 14...intensity of the protein bands to the overall lane. Import the gel image into ImageJ. Select File . Select... each peak by selecting the center of the peak. Export results as a csv file. In the results table, select...

  4. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ... out of fashion but is still useful and most importantly, effective. In the research world, there is a...plasmids The design of shRNA plasmids requires three important steps: Designing the shRNA sequence Choosing ...

  5. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...purification and minimizes sample loss while also supporting imaging, making it easy to identify dozens or...Integrase Gene Editing (PASSIGE), their previously-reported technique for precisely integrating large DNA ...

  6. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...cells to make CAR T cells. The extracellular scFv portion of the receptors recognizes an antigen of interest...interest while the intracellular T cell receptor portion of the CAR facilitates signal transduction and release...

  8. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...option for the experiment above, but it’s also important not to load yourself up with too many tasks. If...ll discuss in the planning section below, it’s important to dedicate specific amounts of time to specific...

  9. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...merely co-opted toward that aim (i.e. sacB). Importantly, to prevent activation of toxins before the second...genome editing in Escherichia coli." Scientific reports 5 (2015): 15096. PubMed PMID: 28060411. Thomason...

  10. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ... areas of microbial research like metabolism. Transport, biofilms, and much more. Because of it’s BSL-...designed for use in Arabidopsis. There are also important tips for plant transformations and the use of ...

  11. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...protocol is a good place to get started.  One important consideration when deciding whether you can do...specific considerations to keep in mind. Most importantly, make sure you are using primary antibodies from...

  12. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...better to reduce off-target effects, but it’s important to not let ‘perfection’ get in the way of ‘good... score their probability of off-target events: CRISPOR, Cas-OFFinder, CCTop, and many more. While such...

  14. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...some DNA during the gel purification step, it is important to digest plenty of starting material. We recommend...be cut with both enzymes, and therefore it is important that the digest go at least 4 hours and as long...running a gel for purification purposes it is important to have nice crisp bands and to have space to ... your favorite gel purification method, it is important to determine the concentration of recovered DNA...ratios of recipient plasmid to insert. It is also important to set up negative controls in parallel. For instance...

  15. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...a pressurized buffer called sheath fluid and transported through tubes or capillaries to a laser. As the...tag your protein of interest with a fluorescent reporter on the N- or C-terminus of the protein. One downside...

  16. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...around the band as possible. To do so, it is often important to take the excised band, lay it down on the UV...sides with the razor blade. This is especially important during the DNA purification step, as many kits... fragment. The weight of the gel is directly proportional to its liquid volume and this is used to determine...manufacturer's instructions. Note: It is usually important to determine the concentration of the DNA that...

  17. Protocol - Bacterial Transformation

    Type
    Protocol
    .... Transformation of bacteria with plasmids is important not only for studies in bacteria but also because...amplification. Higher efficiency cells are more important if you will be transforming with very small amounts...critical for Ampicillin resistance but is much more important for other antibiotic resistances. Plate some or... do this you will need to have access to an electroporator and the appropriate cuvettes. Follow the manufacturer's...

  18. Using a Light Microscope Protocol

    Type
    Protocol
    ...light to detect small objects. Two of the most important properties of a microscope are magnification (...see several pieces of information, but the most important (for now) is the magnification power, such as ...be sure to carry it with two hands - one hand supporting the base and the other holding the arm - or use...your images in the future. Conclusion Like any important laboratory instrument, you should be sure to take...

  19. Lentivirus ddPCR Titration

    Type
    Protocol
    ...EX0276-1 Benzonase 250 U/µl, Millipore #71205-3 Polybrene 10 mg/mL, Millipore, TR-1003-G Molecular Biology...place the PCR plate with the foil onto the metal support block. Place the block in the plate sealer and ... reading. When the droplet reading is complete, export the data from all wells as a CSV file which will...

  20. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...desired insert. If you are in this situation, it is important to treat the digested vector backbone with a phosphatase...setting up the ligation reaction itself, it is important to determine the amount of cut insert and vector...being sure to increase the amount of buffer proportionally. 1μL of ligase should be sufficient for larger...
Showing: 701 - 720 of 921 results