We narrowed to 1,006 results for: RING
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TypeBlog Post...intramolecular DNA recombination. This naturally occurring process is mediated by recombinases like RecA ...bacterial selection marker and origin of replication. During bacterial culture, only the plasmid with the antibiotic...
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A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog Post...spatially confined experiments as well as those requiring reversibility that you cannot do with AraC,” says...MA, Ernst L, Khammash M, Di Ventura B (2021) Engineering AraC to make it responsive to light instead of... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog Post...translation of CRISPR technology, but they would also bring benefits to researchers conducting basic or early... Mary Gearing... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog Post...plan to continue updating the feature database. Powering the plasmid maps on Addgene.org with SnapGene ... powered plasmid maps Learn how we use SnapGene during the plasmid quality control process Check out full... -
The Strength of Story Telling
TypeBlog Post...talks and interviews. They even tell short stories during weekly lab meetings. When scientists tell good ...when appropriate. It might not be scientific to bring emotional discovery into a scientific story, but... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog Post... ITR integrity and prevent unwanted mutations? During propagation, mutants rapidly replicate in a growth...help to reduce the likelihood of ITR deletions occurring. How do I validate my ITR sequences? Next-generation... -
Virtual Science Conference Coming Up? Three Addgenies Share Their Experience
TypeBlog Post...Everyone Workshop included this interactive activity during their session. Image from Reproducibility for Everyone....Viewing a poster on a screen is challenging, requiring you to zoom in to different parts of the poster... -
The Importance of High Titer for AAV Transductions
TypeBlog Post...therefore titer may not need to be as high when delivering receptor constructs with AAV. In the case of ...less particles to be released into suspension and lowering the number of viral particles that can be harvested... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog Post...tech at Addgene, part of my job, in addition to preparing DNA and generating/replenishing kits, is to find...also gave us a few goodies which included an NEB drawstring backpack and a double wall insulated glass to... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog Post...Perrimon, Targeted gene expression as a means of altering cell fates and generating dominant phenotypes....Environmental Stress Causes Lethal Neuro-Trauma during Asymptomatic Viral Infections, Cell Host Microbe... -
Creating accessible biology activities in schools with BioBits
TypeBlog Post... a 4th year PhD student at MIT in Biological Engineering and is interested in developing synthetic biology...research Learn how the Arabidopsis research center brings plant science to life Resources on Addgene.org... -
DAP arrays for multiplex gene editing
TypeBlog Post...multiplex strategies have been facing difficulties in pairing with base editing and prime editing approaches....also enable more convenient multiplex genome engineering of crops or allow for sophisticated CRISPR screenings... -
How to Lead a Great Meeting
TypeBlog Post...are there. Make the most of everyone’s time by considering the level of meeting you need. Project Meetings...review the agenda and add anything that is missing. During the meeting, take notes on the agenda points. At... -
Management for Scientists: Delegating is Key
TypeBlog Post...delegating for success. Meet regularly before during and after the assignment to discuss goals, expected...the Management for Scientists Series Learn How Volunteering at a Professional Organization Can Help You ... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog Post...Add ethidium bromide (EtBr) to your gel before pouring it. EtBr binds to the DNA and allows you to visualize... topics such as how to set up your digests and pouring/running a DNA gel. More Plasmid Eductional Resources... -
Plasmids 101: Mammalian Vectors
TypeBlog Post...GFP is often used as a reporter and we will be covering this ever-versatile fluorophore in a later post...selection markers are listed below: Name Gene Conferring Resistance Cell Types* Mode of Action** Working... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog Post...are some good practice tips on adjusting voltage: During data acquisition, make sure that the signal for...upregulation or downregulation are easier to identify by comparing peak positions to the base expression. When compared... -
Antibodies 101: ChIP
TypeBlog Post... ChIP is an incredibly useful application for exploring these interactions, and can give valuable insight...and the Three Steps (Crosslinking, Fragmenting, Capturing), if you ask me. Each of the three steps need... -
Antibodies 101: Monoclonal Antibodies
TypeBlog Post...you are studying a protein that may bind a ligand during your experiment, then you’d want to make sure that... scientists can freeze hybridoma cells before culturing or they can clone the genes from the hybridoma... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog Post...molecules to either activate or inhibit neuronal firing. As GPCRs are the largest class of signal transducing... and have different effects on neuron activity (firing or inhibition). AAV preps from DREADD plasmids ...