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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,009 results for: RING

Showing: 701 - 720 of 1009 results

  1. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...transduction?  Transduction is the process of transferring foreign nucleic acid into a cell by a viral ...Expressed episomally long-term? Expressed short-term? Answering these questions will help guide you to the right...

  2. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ... high G+C content genes, as validated through monitoring the expression of fluorescent proteins and oxytetracycline...-nucleotide modifications to edit DNA without requiring a double-stranded break. The Xiao Wang Lab has...

  7. The Strength of Story Telling

    Type
    Blog Post
    ...talks and interviews. They even tell short stories during weekly lab meetings. When scientists tell good ...when appropriate. It might not be scientific to bring emotional discovery into a scientific story, but...

  8. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...infection, that is. Thankfully, through the use of engineering controls — changes made to the virus that eliminate...and Man: A History of Interactions (pp. 23–40). Springer International Publishing. https://doi.org/10.1007...

  10. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...therefore titer may not need to be as high when delivering receptor constructs with AAV. In the case of ...less particles to be released into suspension and lowering the number of viral particles that can be harvested...

  11. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...tech at Addgene, part of my job, in addition to preparing DNA and generating/replenishing kits, is to find...also gave us a few goodies which included an NEB drawstring backpack and a double wall insulated glass to...

  14. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...multiplex strategies have been facing difficulties in pairing with base editing and prime editing approaches....also enable more convenient multiplex genome engineering of crops or allow for sophisticated CRISPR screenings...

  15. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ... ITR integrity and prevent unwanted mutations?  During propagation, mutants rapidly replicate in a growth...help to reduce the likelihood of ITR deletions occurring.  How do I validate my ITR sequences?  Next-generation...

  16. How to Lead a Great Meeting

    Type
    Blog Post
    ...are there. Make the most of everyone’s time by considering the level of meeting you need. Project Meetings...review the agenda and add anything that is missing.  During the meeting, take notes on the agenda points. At...

  17. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...delegating for success.  Meet regularly before during and after the assignment to discuss goals, expected...the Management for Scientists Series Learn How Volunteering at a Professional Organization Can Help You ...

  19. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...GFP is often used as a reporter and we will be covering this ever-versatile fluorophore in a later post...selection markers are listed below: Name Gene Conferring Resistance Cell Types* Mode of Action** Working...

  20. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ...are some good practice tips on adjusting voltage: During data acquisition, make sure that the signal for...upregulation or downregulation are easier to identify by comparing peak positions to the base expression. When compared...
Showing: 701 - 720 of 1009 results