We narrowed to 1,006 results for: RING;

...translation of CRISPR technology, but they would also bring benefits to researchers conducting basic or early... Mary Gearing...

...plan to continue updating the feature database. Powering the plasmid maps on Addgene.org with SnapGene ... powered plasmid maps Learn how we use SnapGene during the plasmid quality control process Check out full...

...talks and interviews. They even tell short stories during weekly lab meetings. When scientists tell good ...when appropriate. It might not be scientific to bring emotional discovery into a scientific story, but...

...Everyone Workshop included this interactive activity during their session. Image from Reproducibility for Everyone....Viewing a poster on a screen is challenging, requiring you to zoom in to different parts of the poster...

...multiplex strategies have been facing difficulties in pairing with base editing and prime editing approaches....also enable more convenient multiplex genome engineering of crops or allow for sophisticated CRISPR screenings...

... a 4th year PhD student at MIT in Biological Engineering and is interested in developing synthetic biology...research Learn how the Arabidopsis research center brings plant science to life Resources on Addgene.org...

...therefore titer may not need to be as high when delivering receptor constructs with AAV. In the case of ...less particles to be released into suspension and lowering the number of viral particles that can be harvested...

...tech at Addgene, part of my job, in addition to preparing DNA and generating/replenishing kits, is to find...also gave us a few goodies which included an NEB drawstring backpack and a double wall insulated glass to...

...Perrimon, Targeted gene expression as a means of altering cell fates and generating dominant phenotypes....Environmental Stress Causes Lethal Neuro-Trauma during Asymptomatic Viral Infections, Cell Host Microbe...

...are there. Make the most of everyone’s time by considering the level of meeting you need. Project Meetings...review the agenda and add anything that is missing.  During the meeting, take notes on the agenda points. At...

...delegating for success.  Meet regularly before during and after the assignment to discuss goals, expected...the Management for Scientists Series Learn How Volunteering at a Professional Organization Can Help You ...

...Add ethidium bromide (EtBr) to your gel before pouring it. EtBr binds to the DNA and allows you to visualize... topics such as how to set up your digests and pouring/running a DNA gel.  More Plasmid Eductional Resources...

...GFP is often used as a reporter and we will be covering this ever-versatile fluorophore in a later post...selection markers are listed below: Name Gene Conferring Resistance Cell Types* Mode of Action** Working...

... change allowing high affinity binding to SspB, brings together the split botulinum neurotoxin halves ... to diffuse in and out but prohibit water from entering their hollow interiors. The Shapiro lab discovered...

...molecules to either activate or inhibit neuronal firing. As GPCRs are the largest class of signal transducing... and have different effects on neuron activity (firing or inhibition). AAV preps from DREADD plasmids ...

...come up with a new solution.  Now, you may be wondering how this all fits in with science. Well, I’ll ...a software product manager does, you might be wondering what the day to day looks like. Check out the ...

... ChIP is an incredibly useful application for exploring these interactions, and can give valuable insight...and the Three Steps (Crosslinking, Fragmenting, Capturing), if you ask me.  Each of the three steps need...

...the body. Because calcium levels change rapidly during neuronal activity, scientists can use GECIs to ...shows: Higher ΔF/F0 (the measure of signal change during calcium events), Greater brightness, Less signal...

...are some good practice tips on adjusting voltage: During data acquisition, make sure that the signal for...upregulation or downregulation are easier to identify by comparing peak positions to the base expression. When compared...

...you are studying a protein that may bind a ligand during your experiment, then you’d want to make sure that... scientists can freeze hybridoma cells before culturing or they can clone the genes from the hybridoma...