We narrowed to 1,006 results for: RING;
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TypeBlog Post...plan to continue updating the feature database. Powering the plasmid maps on Addgene.org with SnapGene ... powered plasmid maps Learn how we use SnapGene during the plasmid quality control process Check out full...
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The Strength of Story Telling
TypeBlog Post...talks and interviews. They even tell short stories during weekly lab meetings. When scientists tell good ...when appropriate. It might not be scientific to bring emotional discovery into a scientific story, but... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog Post... high G+C content genes, as validated through monitoring the expression of fluorescent proteins and oxytetracycline...-nucleotide modifications to edit DNA without requiring a double-stranded break. The Xiao Wang Lab has... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog Post...intramolecular DNA recombination. This naturally occurring process is mediated by recombinases like RecA ...bacterial selection marker and origin of replication. During bacterial culture, only the plasmid with the antibiotic... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog Post...spatially confined experiments as well as those requiring reversibility that you cannot do with AraC,” says...MA, Ernst L, Khammash M, Di Ventura B (2021) Engineering AraC to make it responsive to light instead of... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog Post...Perrimon, Targeted gene expression as a means of altering cell fates and generating dominant phenotypes....Environmental Stress Causes Lethal Neuro-Trauma during Asymptomatic Viral Infections, Cell Host Microbe... -
How to Lead a Great Meeting
TypeBlog Post...are there. Make the most of everyone’s time by considering the level of meeting you need. Project Meetings...review the agenda and add anything that is missing. During the meeting, take notes on the agenda points. At... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog Post... ITR integrity and prevent unwanted mutations? During propagation, mutants rapidly replicate in a growth...help to reduce the likelihood of ITR deletions occurring. How do I validate my ITR sequences? Next-generation... -
Creating accessible biology activities in schools with BioBits
TypeBlog Post... a 4th year PhD student at MIT in Biological Engineering and is interested in developing synthetic biology...research Learn how the Arabidopsis research center brings plant science to life Resources on Addgene.org... -
The Importance of High Titer for AAV Transductions
TypeBlog Post...therefore titer may not need to be as high when delivering receptor constructs with AAV. In the case of ...less particles to be released into suspension and lowering the number of viral particles that can be harvested... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog Post...tech at Addgene, part of my job, in addition to preparing DNA and generating/replenishing kits, is to find...also gave us a few goodies which included an NEB drawstring backpack and a double wall insulated glass to... -
Virtual Science Conference Coming Up? Three Addgenies Share Their Experience
TypeBlog Post...Everyone Workshop included this interactive activity during their session. Image from Reproducibility for Everyone....Viewing a poster on a screen is challenging, requiring you to zoom in to different parts of the poster... -
DAP arrays for multiplex gene editing
TypeBlog Post...multiplex strategies have been facing difficulties in pairing with base editing and prime editing approaches....also enable more convenient multiplex genome engineering of crops or allow for sophisticated CRISPR screenings... -
Management for Scientists: Delegating is Key
TypeBlog Post...delegating for success. Meet regularly before during and after the assignment to discuss goals, expected...the Management for Scientists Series Learn How Volunteering at a Professional Organization Can Help You ... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog Post...Add ethidium bromide (EtBr) to your gel before pouring it. EtBr binds to the DNA and allows you to visualize... topics such as how to set up your digests and pouring/running a DNA gel. More Plasmid Eductional Resources... -
Plasmids 101: Mammalian Vectors
TypeBlog Post...GFP is often used as a reporter and we will be covering this ever-versatile fluorophore in a later post...selection markers are listed below: Name Gene Conferring Resistance Cell Types* Mode of Action** Working... -
Luminescent Imaging with Nano-lanterns
TypeBlog Post...to rely on differences in emission wavelength. Filtering the emission wavelength partially separates the... Mary Gearing... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog Post...are some good practice tips on adjusting voltage: During data acquisition, make sure that the signal for...upregulation or downregulation are easier to identify by comparing peak positions to the base expression. When compared... -
Plasmids 101: Inducible Promoters
TypeBlog Post...shock-inducible Cre and Cas9 for easy genome engineering in species like C. elegans and Drosophila. Light... Mary Gearing... -
New and Upcoming Viral Vectors - May 2020
TypeBlog Post...acts through the Gq pathway and promotes neuronal firing. Plasmid Serotype Name Depositor 44361 AAV1...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...