We narrowed to 1,045 results for: STI;

...promoters, origins, tags, and certain ORFs. Their sophisticated detection algorithm easily and precisely identifies...Sequence” tabs. Primers This tab contains a table listing commonly used primers detected in a given nucleotide...thanks to you (our community of scientists) for suggesting these changes, and the SnapGene and Addgene software...

...an important feature of a fluorophore. The key question is how the brightness is best defined or determined... cells within the dish. This is caused by the stochastic nature of plasmid uptake by cells. To correct...precise determination of brightness in cells, under realistic experimental conditions. By determining the brightness...

...began with only a few inventory items offered domestically, but by the end of 2016, we expanded our viral...vector developed by Feng Zhang’s lab at the Broad Institute (Sanjana et al., 2014). It is a dual-vector system... Carolina, Chapel Hill. These can be used to investigate the effects of activating particular signaling...

...recombineering to create insertions, deletions, and substitutions - at the same efficiency as Gibson Assembly ... This method can work well, but poor template digestion can result in a high number of background colonies...fragment In vitro Assembly No No Yes Yes DpnI Digestion Yes Yes No No Purified Enzymes Required No No...

...have something in common. Namely, the scientific question you are interested in and this is a great ice ...relationships. Make a list of experimental problems or questions you are pondering and be ready to talk about them...scientists your own age. Some will be friendly so stick with those. Don’t miss social opportunities, but...

...? Sometimes, the funders of your research may stipulate that your article must be published in an open...scope of the journal you are sending it to. It’s wasting everybody’s time if you don’t. Add a clear cover...get writing! Let us know if you have any other questions about writing and publishing your manuscript, ...

...discussion with resources. One of the most common questions I get is, “What are we going to talk about?” There...internet, but start by downloading my handy list of suggestions.  Get started. Have regular meetings. Experiment...ideas from your own mentoring group or with any questions about mentoring. Read the other posts in the series...

...there and what are they doing. To address these questions, biologists often use next-generation sequencing...microbiota and host biomarkers In clinical and diagnostic labs, deep sequencing and qPCR methods are too...Jeffrey Barrick’s labs at The University of Texas at Austin recently developed the Bee Microbiome Toolkit (...

...- These fragments are produced by proteolytic digestion that retains an antibody’s disulfide bonds within...resulting product is a mini-Y with both Fab regions still attached to one another. Size: ~110 kDa. Fab' - ...- These fragments are produced by proteolytic digestion above the hinge-region, which results in two independent...

...target tissue, two weeks is the standard ballpark estimation. Once expressed, viral cargo is usually quite...a Postdoctoral Fellow in Connie Cepko’s Lab investigating neuronal circuits of the retina using viral ...12349826. Xiong, Wenjun, and Constance Cepko. "Distinct expression patterns of AAV8 vectors with broadly...

... to make a plasmid. Doench’s lab in the Broad Institute has created hundreds of thousands of plasmids,...also a great way to get to know people doing interesting and relevant work in your field! However, there...my ability to tell. But even from these rough estimates, it’s clear that community engagement with and...

...tet-off turns off constitutively expressed proteins while tet-on turns on constitutively silenced proteins...know what the job requirements are. Here are the questions you should be asking. What kind of control? On...

...initiates single stranded DNA degradation. Cas3 DNA digestion can proceed up to multiple kilobases, but only...that it generates staggered breaks, also known as sticky ends. Cas12a cleavage results in 5 bp overhangs...efficient, multiplexable RNA knockdown. Cas13 has also stirred up interest for its potential as a viral therapeutic...

... admittedly, somewhat meaningless) practice of listing “Submitted” articles in their CV or grant applications...directly alongside the preprint while the work is still in progress or awaiting a final publication in a...Motivations, concerns and selection biases when posting preprints: a survey of bioRxiv authors. bioRxiv...

...core mission. Plasmid distribution is amazingly still growing at >9% every year (12% in 2018) since 2005... The viral vector team is publishing research, hosting conferences, and are expanding our catalog of materials...community of scientists who support us by depositing, requesting and spreading the word. Our blog and site ...

...this though) about the fact that containers are distinct from their contents. You should be tracking both...tray or a freezer. Some are disposable, like a plastic tube. Talk with your lab team about how they would...getting up and running and more time working on interesting biotechnology code.There are valid technical ...

...one-pot reactions with straightforward restriction digestion and ligation steps to build barcoded lentiviral...pool of lentiviral plasmids. Multiple rounds of digestion and ligation result in plasmid libraries containing...tailored to address the users’ specific research questions. CombiGEM has been successfully applied to functionally...

...You’ve done great research and made interesting discoveries. You’ve analyzed the data and generated beautiful...should be easily navigable and efficient; your questions, data, and conclusions should be clearly identified...spacing between them so that they are easily distinguished. The UCLA Library offers some Powerpoint based...

...highlighted new tools but also included time for questions and a group discussion on the best strategies ... the Arizona State University (ASU) Biodesign institute developed a 3’UTR database specifically for C.... to activation sequences upstream of genes to stimulate expression (Wang et al., 2016). In 2018, the lab...

...include Swiss Webster and Harlan (Hsd) National Institutes of Health (NIH) Swiss. Genetically engineered...injection of a targeting vector alone, some challenges still exist. Nucleases may cause off-target effects on...the transgene in specific cells or under certain stimuli. Cre recombinase catalyzes the recombination of...