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We narrowed to 964 results for: TES

Showing: 701 - 720 of 964 results

  1. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...restriction sites to flank your insert : You can use PCR Based Cloning and add restriction sites to the ends...as long as it is already bounded by restriction sites that are also present in the same orientation on...Analyzer , allow you to identify which restriction sites are present in a given sequence. When selecting ...recipient plasmid as well as a specifically designed test digest later to verify that the insert was cloned...a version of your insert flanked by restriction sites compatible with the recipient plasmid's MCS. However... within your insert. Adding desired restriction sites to your recipient plasmid : You can modify the MCS...

  2. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...dilution is appropriate). Quality of duplicates: Exclude duplicates from analyses if there is more than...-log of the expected titer of the samples being tested. Always include a No Template Control (NTC), i....small aliquot of each standard (enough for 1 or 2 plates) and store at -20 °C. Once a standard is thawed...the assay multiple plasmids containing ITR were tested. Plasmid #59462 is one plasmid that gave reliable...consistent results. Use the recommended plasmid, or test multiple plasmids to find a suitable one. Some labs...second peak at a temperature of ~70–75 °C usually indicates the presence of primer dimers which can increase...

  3. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ... including the recent homepage and search tool updates. Now, let’s take a look at the least visible part...behind Addgene’s new search  Like millions of other websites, we built our new search engine atop Elasticsearch...

  4. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...site of interest, which nCas9 nicks and then dissociates from. PolI3M then binds the nicked DNA, and extends..., where it nicks its target DNA (left). Cas9 dissociates and PolI binds (middle) and extends from the ...

  7. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...small molecule realm is typically not feasible to test in any given screening experiment. Thus, a limited...require there to be a culture condition which eliminates manipulations that do not affect the function...

  8. Antibodies 101: ChIP

    Type
    Blog Post
    ...  Fig. 1: Schematic overview of ChIP. Image courtesy of Shengliu via Wikicommons.   Okay, let’s...celebrating a successful ChIP experiment! Image courtesy of https://www.wannapik.com/   Even though...

  9. Hot Plasmids - February 2022

    Type
    Blog Post
    ...2: comparison of Cas 9 vs. Cas 12a expression cassettes when encoding multiple guides. While two guides... and fusion proteins, such as Cas9 and Cas12a, creates challenges for in vivo delivery and cell engineering...

  10. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...security-critical bugs, and contribute to constant updates of the software. In hardware, this process is often...are as follows: Detailed documentation, which facilitates the reproduction of the structure in a similar...

  11. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    ...Better performance = better data In side-by-side tests with popular red and yellow GECIs, OCaMP shows: ...events are indicated below the trace; asterisk indicates a single AP. B) Averaged fluorescence responses...

  12. Clear and Accessible Writing

    Type
    Blog Post
    ...supernatant through each layer. The right panel illustrates a gradient tube before and after ultracentrifugation...ultracentrifugation. The viral supernatant migrates through increasingly dense layers until the macromolecule density...

  13. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...sequence containing transcription factor binding sites can increase transgene expression and transduction...validate ITR sequences as these enzymes cut at known sites within the ITR. However, small deletions and point...

  14. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...parts: the receptor itself and the G-proteins it associates with.  Receptor: GPCRs are also known as seven...transducing receptors in the brain they are ideal candidates for use in chemogenetics. To create chemogenetics...

  15. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ... and have worked closely with us to functionally test our virus. Ed Boyden’s lab from MIT and Alla Karpova... methods, performs extensive quality control, generates  educational materials, and provides viral vector...

  17. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ... fits the plate. Incubate plates at 37 ℃ for 18 hours. Day 2 Observe plates for colony formation. Shown...Antibiotic Plating You may also like... Making LB Agar Plates Bacterial Transformation Recovering Plasmid DNA...source) Incubator Reagents 6 cm diameter LB/agar plates without antibiotic High concentration (100 mg/mL... the plate at room temperature for at least 30 minutes with the lid on to give the antibiotic time to ... with smaller size than the 1 mg/mL and 2 mg/mL plates and effective selection. Selection Curve of Transformed...

  18. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...and isolated with antibiotic selection. The team tested this system in vitro and in vivo. They identified...fluorescent proteins into one bright particle that segregates into one cell during cell division. To achieve...

  19. Addgene: The First Twenty Years

    Type
    Blog Post
    ...theaters, the Razr was the hottest phone on the market, and two new websites, Facebook and Gmail, were ...

  20. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...load The Wikipedia definition of cognitive load states: “cognitive load refers to the used amount of working...even the most enthusiastic of learners.  What contributes to cognitive load?  Everything! Here are some...
Showing: 701 - 720 of 964 results