We narrowed to 771 results for: VARS

1. Plasmids 101: Knockout/Knock-In Plasmids

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   ...depositors have developed new CRISPR-based methods for various knock-ins, discussed in our CRISPR 101 eBook. ...

2. Addgene's Cancer Collection Pages Connect the Research Community

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   .... Despite the prevalence of Ras mutations in a variety of cancers, Ras proteins have stumped the pharmaceutical...

3. Google Forums Round Up: First Impressions of NgAgo

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   ...Update (November 18, 2016): Researchers from a variety of institutions recently reported their inability...

4. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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   ...biosensors were made with CFP and YFP, cyan and yellow variants of EGFP equally capable of dimerizing as EGFP....

5. PITChing MMEJ as an Alternative Route for Gene Editing

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   ..., and the balance of NHEJ, HDR, and MMEJ repair varies from organism to organism. MMEJ doesn’t yield a...

6. Anatomy of a Plasmid Page at Addgene

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   ...Check the Price of course! The price of some items vary, for example, plasmid kits are avaliable for less...

7. Genome-wide Screening Using CRISPR

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   ...modifying the genome. If you'd like to brush up on the various CRISPR technologies, check out Addgene's CRISPR...

8. Light Sheet Fluorescence Microscopy

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   ... reviews on several other LSFM configurations’ various features see (4,7).   Light sheet fluorescence ...

9. Viral Vectors 101: The Retroviral Lifecycle

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   ...different genomic sequence from one another) — by various mechanisms, only one of the two strands’ DNA counterparts...

10. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    ...BiFC experiments. The ideal control is a mutated variant of one or both of your PIPs in which the protein-interaction...

11. Isolating Genomic Regions of Interest with the CRISPR System

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    ...researchers have begun to make important discoveries in various biological fields. In 2015, the Fujii Lab identified...

12. Droplet Digital PCR for AAV Quantitation

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    ...ddPCR. One issue with qPCR is that the results can vary by a factor of 2. This means that if you set up ...

13. Tips for arabidopsis transformation

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    ... or affinity tags (HA, FLAG). There are also a variety of plant selection markers (basta, hygromycin, ...

14. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    ...systems consist of a genetically encoded enzyme variant tag that reacts specifically and irreversibly with...

15. CRISPR Antimicrobials

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    ... based on the well-studied T7 phage, to target various types of bacteria. In mixed populations, their ...

16. Plasmids 101: Protein tags

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    ...between column runs. The TEV protease itself, with various mutations used to increase its stability activity...

17. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

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    ...ended up being very straightforward to engineer various cell lines from different tissues and mammalian...

18. Save Time with Transient Plant Leaf Transformations

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    ...simple and easy technique to implement. Success might vary between experimenters. Vacuum infiltration (Mortensen...

19. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

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    ...manipulation. The ReMOT control system (Receptor-Mediated Ovary Transduction of Cargo)  delivers Cas9 RNP to the...

20. Tips for Writing a Good Cover Letter

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    ...application. Even within an organization, there can be variance. At Addgene, our content team, where writing is...