We narrowed to 968 results for: abo
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TypeBlog Post...changes. Using this road map, and what was known about previously characterized TFs, the Zhang group were...Additional Resources on the Addgene Blog Learn More about Lentivirus Figure out How to Get the Most from Your...
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With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog Post...conduct the screen. To help alleviate some of this labor for our users, we now provide the Brunello (targeting... hear from you if you have used or are thinking about using our viral services. Please send any questions... -
Management for Scientists: Delegating is Key
TypeBlog Post...Medical School researcher Connie Cepko to learn about her management style. Recipes for delegation success...accountability? What types of issues do you want to know about right away? Provide adequate support. The manager... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog Post...immunofluorescent images like in this paper (2). Metabolic assays such as Seahorse can be employed to look...Additional Resources on the Addgene Blog Learn about Using Cre in Drosophila Read Other Genome Engineering... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog Post... single amino acid. Without further information about properties like photostability or aggregation tendency...Fluorescent Proteins for Multicolor Imaging Learn about Fluorescent Protein Biosensors Additional Resources... -
Multiplex Genome Editing with CRISPR-Cpf1
TypeBlog Post...targets compared to Cas9. Read on to learn more about Cpf1 multiplexing. For an in-depth review of Cpf1... Addgene. If you have any questions or thoughts about Cpf1 multiplexing, leave them in the comments below... -
Screening for Successful Genome Editing with Digital PCR
TypeBlog Post...advantages over mismatch nuclease assays that will be elaborated below. Digital PCR Digital PCR (dPCR) involves...type targets. Comparatively, detection limits of about 5% mutant targets have been reported for mismatch... -
Top Requested Lentivirus and AAV of 2016
TypeBlog Post...Resources on the Addgene Blog Learn More About Lentivirus Learn more About AAV Get the Scoop on Our Viral Service... -
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection
TypeBlog Post...blog to learn about making your own competent cells and our protocols page to learn about bacterial transformation... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog Post... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here. ...can take as long as several days. In 2015, the laboratory of long-time Addgene depositor Alice Ting used... -
New and Upcoming Viral Vectors - May 2020
TypeBlog Post...vector name and serotype you’d like to be notified about. Chemogenetics Plasmid Serotype Name 44362 ...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog Post...interest. Created with biorender.com. The plot above is for a smeary expression and is quite different.... Additionally, you can gate off the histograms above, allowing you to visualize your data as a dot plot... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog Post...packaging methods. The three components described above (envelope, packaging, and transfer) are supplied...gamma-retrovirus. Frequently asked questions about γ-retroviral vectors How do engineered γ-retroviruses... -
AAV Q&A with Tim Miles
TypeBlog Post...labeling in the brain with PHP.eB: A recent CLOVER collaboration injected 1e+13 vg per mouse to get phenotypes...coverage. Please see this paper for more information about PHP.eB brain labeling at various doses. As always... -
Viral Vectors 101: Biosensors
TypeBlog Post...component of a biosensor is the thing that you care about within your cellular or animal system. This ‘thing...for expression and downstream monitoring. Nervous about making a virus for your biosensor? Don’t be! We ... -
Viral Vectors 101: Viral Applications
TypeBlog Post...Viruses as Biological Tools blog can help you learn about the different types of viruses and pick one for ...Addgene.org Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog Post... in science brings stronger networking across laboratory and disciplinary boundaries and can allow for... that are otherwise cut off from the process. About the author: Benedict Diederich received his PhD ... -
From Lab Bench to Leadership: Transitioning into Management as a PhD
TypeBlog Post...clarifying roles, I eased the tension and improved collaboration. The good news: It’s a journey, not a final...of postdocs focusing on molecular biology and metabolism. He now guides fellow PhDs in their careers with... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog Post...questions is the same--methylation! Read on to learn about how DNA methylation may affect your restriction ...be methylated. Using the same example plasmid as above (which has the XbaI site blocked by methylation)... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog Post...with limited packaging capacity. Still confused about the various tRNA expression strategies? No worries... Addgene. If you have any questions or thoughts about this technology, leave them in the comments below...