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We narrowed to 965 results for: app

Showing: 701 - 720 of 965 results

  1. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...location of the cut and subsequent edit. In these applications, location takes priority over cutting efficiency...in double stranded cleavage and base editing applications, with high editing capacity in both contexts...

  2. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...Wickersham et al., 2007).   Summary and RABV applications Use of G deleted RABV in conjunction with restricted...L, Ogawa SK, Vamanrao A, Uchida N. Whole-brain mapping of direct inputs to midbrain dopamine neurons. ...

  4. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...within 1-10nm of each other and be oriented appropriately for energy transfer via dipole-dipole interaction..., which could monitor physical stresses or even apply forces (Gayrard and Borghi 2016) to a selected protein...

  7. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...noticed one of the biggest differences between applying for an academic position versus an industry position...interview is an opportunity to learn something and then apply it to the next interview. Perhaps an interviewer...

  8. Hot Plasmids - October 2020

    Type
    Blog Post
    ... FP genes into C. elegans? By using a split FP approach. Split-wrmScarlet is a new split red FP that is...Hybrid for Multiplexed Editing and screening Applications (CHyMErA) Knockout Libraries express hybrid ...

  10. Tips for Technical Support Calls

    Type
    Blog Post
    ..., I’m confident that these tips and tricks will apply to any biology-related customer support interaction...company. This might seem like a no-brainer, but it happens more frequently than you might think. The Addgene...

  12. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...eclipse any of the CRISPR nucleases in AAV-based applications. Get More Cpf1 Background Here THE ORIGINAL ...the PAM sequence. Many research and clinical applications require HDR over NHEJ, so comparing HDR efficiency...

  13. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...characterization of the location, morphology, and circuit mapping of a targeted neuronal population.    Figure... cell types for imaging and long-range circuit mapping. PubMed PMID: 18614669. PubMed Central PMCID: PMC2593125...

  15. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...something you may have to optimize for your specific application. With mammalian systems, it is standard to do...titer the virus, or go straight into your viral application, like protein production. Titration of baculovirus...

  16. Pooled Library Amplifications

    Type
    Blog Post
    ...to amplify the sample for further downstream applications. As individual libraries can vary significantly...feel free to contact us at [email protected]. Happy amplifying! More resources on the Addgene blog ...

  17. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...waste should be autoclaved before being disposed Happy culturing! Many thanks to our guest blogger, Sana...expression disregulation on HeLa cells." Journal of applied phycology27.6 (2015): 2321-2332. 3. Contamination...

  18. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ... just trying to create a knockout, you might be happy targeting anywhere in an exon. But things get more...editing rates. This PAM flexibility should expand applications that require very specific targeting, like homology...

  19. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...possible consequences of FP aggregation, as Erik Snapp’s blog post shows. To avoid these issues, Subach ...-FT-N1 pTRE-Slow-FT pBAD/HisB-Slow-FT -  Applications of fluorescent protein timers FP timers have...
Showing: 701 - 720 of 965 results