We narrowed to 954 results for: app

1. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

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   Blog Post

   .... “The Postdoctoral Experience Revisited” 2014 Appendix B p93-95). For those advocating in this area, ...developing a strategy to distilling a message for the appropriate audience and communicating that message in various...

2. Cpf1 Update: Comparison to Cas9 and NgAgo

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   Blog Post

   ...eclipse any of the CRISPR nucleases in AAV-based applications. Get More Cpf1 Background Here THE ORIGINAL ...the PAM sequence. Many research and clinical applications require HDR over NHEJ, so comparing HDR efficiency...

3. Plasmids 101: FLEx Vectors

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   Blog Post

   ...characterization of the location, morphology, and circuit mapping of a targeted neuronal population.    Figure... cell types for imaging and long-range circuit mapping. PubMed PMID: 18614669. PubMed Central PMCID: PMC2593125...

4. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

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   Blog Post

   ...to resolve your problem. Your supervisor will appreciate that you took the time to think of solutions,...prioritize your action item list and set some approximate timelines. Be sure that you keep your commitments...

5. Production of Virus in Insect Versus Mammalian Cells

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   Blog Post

   ...something you may have to optimize for your specific application. With mammalian systems, it is standard to do...titer the virus, or go straight into your viral application, like protein production. Titration of baculovirus...

6. Pooled Library Amplifications

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   Blog Post

   ...to amplify the sample for further downstream applications. As individual libraries can vary significantly...feel free to contact us at [email protected]. Happy amplifying! More resources on the Addgene blog ...

7. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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   Blog Post

   ... just trying to create a knockout, you might be happy targeting anywhere in an exon. But things get more...editing rates. This PAM flexibility should expand applications that require very specific targeting, like homology...

8. Fluorescent Proteins 101: Fluorescent Protein Timers

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   Blog Post

   ...possible consequences of FP aggregation, as Erik Snapp’s blog post shows. To avoid these issues, Subach ...-FT-N1 pTRE-Slow-FT pBAD/HisB-Slow-FT -  Applications of fluorescent protein timers FP timers have...

9. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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   Blog Post

   ... disease-associated. Mobile-CRISPRi: a modular approach for CRISPRi transfer Mobile-CRISPRi offers two...single-gene knockdown experiments. Mobile-CRISPRi applications Mobile-CRISPRi expands CRISPRi to non-model ...

10. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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    Blog Post

    ...this series, we’ll discuss the two widely used approaches of somatic cell conversion: conversion via iPSC...Pluripotent Stem Cells: Problems and Advantages when Applying them in Regenerative Medicine. Acta Naturae, 2010...

11. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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    Blog Post

    ...resources are limited or for bioart and teaching applications. Find a chromoprotein for your experiment! ...Scarless Modification of Proteobacterial Genomes. Applied and Environmental Microbiology, 85(16). https:/...

12. 10 Basic tips for mammalian cell culture

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    Blog Post

    ...waste should be autoclaved before being disposed Happy culturing! Many thanks to our guest blogger, Sana...expression disregulation on HeLa cells." Journal of applied phycology27.6 (2015): 2321-2332. 3. Contamination...

13. Light Sheet Fluorescence Microscopy

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    Blog Post

    ...of LSFM microscopes (3,4). After this initial application, Stelzer’s group described the single-plane or...shadows along the illumination path. The shadows appear as stripes in the acquired image (5).  A way to...

14. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    Blog Post

    ... Sometimes it’s a good way to find out what’s happening in the office next to you. There is an active ...sometimes stupid or uncomfortable things that have happened to them. To have that conversation out in the ...

15. Which Fluorescent Protein Should I Use?

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    Blog Post

    ...emission is green or red light. For example, T-Sapphire, LSSmOrange, and LSSmKate. Fluorescent Sensors.... al. (2010) “Fluorescent proteins and their applications in imaging living cells and tissues”  Physiol...

16. Viral Vectors 101: The Retroviral Lifecycle

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    Blog Post

    ...101: Types of Viruses Viral Vectors 101: Viral Applications Resources on addgene.org Addgene's Lentiviral...Liu, H., Xiao, H., Conway, J. A., Hunter, E., & Kappes, J. C. (1997). Functional RT and IN incorporated...

17. Isolating Genomic Regions of Interest with the CRISPR System

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    Blog Post

    ...and retroviral expression system for using this approach in in vivo studies (Fujita et al., 2018). In addition...DUX4, a transcriptional regulator that when inappropriately expressed can cause facioscapulohumeral muscular...

18. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    Blog Post

    ...labels for fixed-cell imaging, not for live-cell applications. Thus, these fluorophores are often bulky and...budget gets a bit thin, small-molecule labeling approaches can provide a fresh infusion of photons for your...

19. Tips from the Repository Trenches: Using Barcodes to Track Samples

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    Blog Post

    ... tube contains information about that tube’s appropriate location. The barcode also identifies the contents... sample tubes that we use match those of the appropriate deposit. Using barcoded tubes is part of Addgene...

20. Virus Protocol - Generating Stable Cell Lines

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    Protocol

    ...expression observed using transient transfection approaches, generating cell lines using lentiviral vectors... tubes, VWR 21008-216 Lentivirus preparation Appropriate antibiotic for selection (e.g. puromycin, blasticidin...lower dilutions depending on your downstream applications. If you’ve titered your virus beforehand, you.... Add 1.5 mL of DMEM complete containing the appropriate antibiotic. This is the beginning of the selection...