We narrowed to 872 results for: cel

...these issues by creating immortal antibody-producing cell lines (hybridomas) that secrete antibodies in culture...reproducible production. However, even clonal hybridoma cell lines can produce more than one mAb (Bradbury et...

...successfully used this plasmid to biotinylate the extracellular domain of the erythropoietin receptor and subsequently... Eye Institute and is particularly interested in cell signaling.     Additional Resources on the Addgene...

...would rather fund excellent people with mediocre ideas than mediocre people with excellent ideas. I am lucky...

...  There are many online guides – including an excellent series of posts by Joachim Goedhart on the Addgene...Associate at Harvard Medical School. He manages the Cell Biology Microscopy Facility, specializing in advanced...

...engineering project at BosLab. Once he optimizes plant cell culture and transgenesis he wants to share his knowledge... in an Open Source Lab: from CRISPR to Cats Stem Cell Models for Disease & Open Science: Interview with...

...as an opportunity to practice communicating.  Excellent scientists learn from advisers and other mentors...help graduate students in all STEM fields become excellent science communicators. To empower graduate students...

...of Professor Richard Murray. Murray’s lab uses cellular extracts to perform transcription and translation...develop and test hypotheses. Students then use cellular extracts as a means to rapidly prototype DNA circuits...

...EvolvR could also be a tool for lineage tracing cells that do not tolerate double-strand DNA breaks by...researchers to trace the progeny of a single starting cell. Additionally, multiplexing of guides allows EvolvR...

...team potential. I often thought that performing a cell culture experiment myself would take less time than...Another team member was highly self-motivated and excelled when given the freedom to explore projects autonomously...

...text for the example image. We’ll wait!  Done? Excellent! We asked our workshop participants to do the ...heard of alt text before, they all came up with excellent alt text for their practice image. We know; we...

...allow scientists to express a gene of interest in a cell or animal model. AAV plasmids contain several important...Integrity on rAAV8 Production Using the Baculovirus/Sf9 Cells System. Hum Gene Ther Methods, 28(5), 277–289. https...

... channels in living cells. Multiple properties of a channelrhodopsin, such as cell localization, kinetics...

... project aimed at understanding how a critical cellular pathway is regulated. You’ve read all the background...expression in knock-out or knock-down down tissues and cell lines; orthogonal strategies that compare antibody...

...force to control protein expression in mammalian cells: easy to use biological tools are abundant! In this...tag prior to introduction of the construct into cells. However, if you would like tunable control of an...

...Different AAV serotypes have preferences for specific cell types. For example, AAV1 and AAV9 serotypes have...most cases with VSV-G that generally mediates broad cell tropism and has a high percentage of neuronal targeting...

...strain, the promoter being used should be tested in cell lines to determine how it impacts the expression...interest. Cre may be expressed globally (in every cell of the mouse) or in a tissue-specific manner (with...

...research (either because of expertise, equipment, or excellence of the institution). Likewise, if you’re staying...Cambridge University where he studied mechanisms of cell division. Recently Seán got the entrepreneurial ...

... attempt to write grants. If you don’t enjoy or excel at writing, an industry postdoc will free you of...Scientist at AstraZeneca. She has a background in cell and developmental biology and is applying this towards...

...As we celebrate twenty years at Addgene, we are sharing stories of our repository’s positive impact on... Sharing this story is a truly special way to celebrate our twentieth anniversary in 2024, and it’s all...

...different substances can be injected, including cells, drugs, DNA, viral vectors, and viruses. All researchers... injections are most often used when injecting a cell suspension to create xenograft models. Restraint...