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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 921 results for: ire;

Showing: 701 - 720 of 921 results

  1. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...about its limited resources. Rather than developing entire new teams in house to solve every problem (although...collaborators’ professional developments without directly contacting them. One great way to transition from...

  2. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...solution. Since this technique is intensive and requires an electron microscope, it is not routinely performed...a few ways:  Absolute quantification by qPCR requires generating a standard curve of known concentration...

  3. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...drawbacks limit the utility of these systems: St1Cas9 requires a very specific PAM sequence that limits the number...SaCas9, Zhang’s group used an approach called BLESS (direct in situ breaks labeling, enrichment on streptavidin...

  4. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ... with a distributor for that country. In China, direct shipments weren’t able to clear import control...., we launched the Addgene blog for topics that required a lot of nuance or detail. By the end of 2023,...

  5. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...as Rep or other initiator proteins, they do not require host proteins for replication and can maintain ...pneumophila, Methylophilus methylotrophus, Myxococcus virescens, M. xanthus, Proteus rettgeri, P. mirabilis, Providencia...

  6. How to Be an Excellent Trainee

    Type
    Blog Post
    ...someone who provides career advice and offers direction on your scientific project. Your scientific mentor...meaningful relationships in your new lab, learn the required techniques, and be ready to take on trainees one...

  9. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...best to start from scratch. Obtain a vial of the desired cell line from a reputable distributor such as ...course of infection. These binding events can be impaired by the electrostatic repulsion of the negatively...

  12. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...electron microscopy (cryo-EM) protocols typically require high concentration and purity of the target molecule...particles (eVLPs) offers an option to transduce cells directly with ribonucleoprotein cargo. eVLPs contain no...

  13. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...forward scatter (FSC) and side scatter (SSC). To acquire these measurements, the cytometer illuminates the... “lymphocyte + single cell + live” population, acquired from the previous gating pipeline, about 53% of...

  14. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...., Huisman, T.M., Verkhusha, V.V. & Hodgson, L. Direct multiplex imaging and optogenetics of Rho GTPases...Piatkevich, K.D. et al. A robotic multidimensional directed evolution approach applied to fluorescent voltage...

  15. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...to support your work. For example, if your work requires specialized equipment, try finding institutes ... institutes were able to provide the resources required for him to meet his goals. Therefore, he turned...

  17. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...Similarly, placing UAS upstream of a transgene permits directed expression of that gene in cells that also express...systems, allowing for more complex control over desired gene expression. Take a look at all of the options...

  19. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...cell that gets the plasmid contains all of the desired gRNAs. This increases the chance that all the edits...editing, and a subsequent curing protocol that requires only 2-3 hours incubation. Kondo Lab multiplexed...

  20. Deep Dive: qPCR

    Type
    Blog Post
    ... you to measure the intensity of signal over an entire cycle. Since signals are produced by the presence... practice, multiplexing is quite difficult and requires significant optimization to ensure the results...
Showing: 701 - 720 of 921 results