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Showing: 701 - 720 of 959 results
  1. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...proteins are also involved in the transcriptional regulation of the AAV p40 promoter which drives Cap expression...promoter. Alternative splicing and differing translational start sites generate the three VP proteins. ...
  2. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ... systems; Cascade shown) combines with a donor template and transposon proteins (Tns) to integrate large...outcompete their brethren, and the researchers isolated these more efficient CASTs (Witte et al., 2025...
  3. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ...endothelial cells via an injection into the systemic circulation. In Old World monkeys it actually targets neurons...forget to use the link below to keep up with the latest in Addgene's viral vector services and resources...
  4. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...cultural importance of these animals to human populations around the world. Here at Addgene, we love camelids...living cells or tissues. sdAbs can also be used to manipulate cellular functions, like in this early example...
  5. Antibodies 101: Affinity Tags

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    Blog Post
    ...bacteria, yeast, mammalian, etc.). To individually isolate a protein, there must be a special feature about...S-Transferase (GST) is a 26 kDa tag which can be isolated with glutathione resin. Like MBP, it can be removed...
  6. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    ...may be most effective. Once drug candidates are isolated, they can be validated for specificity to their...before time and money gets put into them in those later stages. Target identification Not all diseases ...
  7. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...selectable marker. Each insertion line can then be isolated and screened. Depending on the marker, traps can...require the trapping of endogenous enhancers to stimulate the expression of the reporter. The system is ...
  8. Cellosaurus: A Cell Line Information Database

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    Blog Post
    ...LitSuggest Tool Following the “rabbit hole” of related cell lines when annotating a cell line Community...whether a cell line is known to be genetically manipulated, its anatomical origin, if cell line has ever...
  9. Sleeping Beauty Awakens for Genome Engineering

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    Blog Post
    ...use of viruses is also important for social and regulatory reasons. 1.) The transposon consists of a mirrored...your gene-of-interest, either constitutively or regulated by doxycycline. The Sleeping Beauty transposase...
  10. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...backbones) are frequently used in molecular biology to isolate, multiply, or express the insert they carry in ... system is great for overexpressing YGOI in an isolated, controlled environment. In most cases bacterial...
  11. Science Careers: Unruly Interests Feed Many Paths

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    Blog Post
    ... projects studying developmentally programmed regulation of DNA replication and gene transcription during... useful and productive, forming a different constellation of interests and skills than what fits for applied...
  12. A Tour of Addgene's Most Popular Pooled Libraries

    Type
    Blog Post
    ...which stands for Multiplexed Overexpression of Regulatory Factors. The Feng Zhang lab developed this library...Element. This library consists of broad host range regulatory sequences ready to be assembled with other elements...
  13. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...viral vector repository with a nice cup of hot chocolate is absolutely delightful. Grab a hot beverage ...forget to use the link below to keep up with the latest in Addgene's viral vector services and resources...
  14. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...genomes can be transcribed into mRNA, directly translated into proteins, or, in the case of retroviruses...which is then transcribed into mRNA before being translated into proteins. RNA viruses replicate their genomes...
Showing: 701 - 720 of 959 results