We narrowed to 968 results for: lat
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  TypeBlog Post...is pictured here…she started from a blank white slate and developed this over 30 minutes, which was fun...this type of experimentation to the eventual translation to the clinic and cures. There is no doubt that...
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  Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout ScreeningTypeBlog Post...game for anyone looking to quickly and easily manipulate specific genes. But what if you want to study...said they were able to produce a very diverse population of cells within a lab dish in a matter of a few...
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  Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control ProcessTypeBlog Post...be located. By easily using BLAST to check the translated amino acid sequence from within Snapgene, we ...size of the plasmid and the insert. By using the “Simulate agarose gel” option with the desktop version of...
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  ReFigure: Save Scientific Figures into Dashboards and Share Your InsightsTypeBlog Post... to discover your insights on a topic. Beyond collating other’s data, you can even include your own data...patients was published, Zika researcher Christa Osuna collated Zika case studies, making a meta analysis available...
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  Oh, The Places You Can Go: Careers in Science Communication - Curriculum DevelopmentTypeBlog Post... part of the job, but Caitlin doesn’t work in isolation. “A lot of what we do is work with students who...theses, and occasionally posting something science-related on Facebook. “I think this helped me say with confidence...
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  Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!TypeBlog Post...identified proteins and enzymes. These compounds will later get tested for their potential agricultural and ...agriculture. For this project the expression of isolated bacterial proteins can be achieved using a bacterial...
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  Addgene's a Nonprofit? Nonprofit Awareness Day 2017TypeBlog Post...confidentiality where it is appropriate. This grows our relationship with our community members. We also find that...Mission Meet the Addgene Leadership Team Get the Latest News from Addgene ...
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  Bringing Sustainable Practices to the Lab: RecyclingTypeBlog Post...researchers represent a minority of the world’s population, we account for an estimated 2% of global plastic...it has to be packaging (no coated tissue culture plates, for example). Check the link for more details ...
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  Viral Vectors 101: Parts of the AAV Packaging PlasmidTypeBlog Post...proteins are also involved in the transcriptional regulation of the AAV p40 promoter which drives Cap expression...promoter. Alternative splicing and differing translational start sites generate the three VP proteins. ...
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  What's the Best Way to Elute and Store Your Plasmid DNA?TypeBlog Post...inactivation, protein degradation, and/or the use of chelating agents that can be introduced in the purification...time. Tris buffer controls the pH, while the EDTA chelates any divalent cations like Mg2+, preventing the...
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  Fostering communication in remote collaborationsTypeBlog Post...person, and helps improve your interpersonal relationships with people you’re collaborating with remotely...external collaborations Browse our science career related blog posts ...
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  CASTing Off for New Shores in Human Genome EditingTypeBlog Post... systems; Cascade shown) combines with a donor template and transposon proteins (Tns) to integrate large...outcompete their brethren, and the researchers isolated these more efficient CASTs (Witte et al., 2025...
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  New Viral Vectors - Fall 2024TypeBlog Post...endothelial cells via an injection into the systemic circulation. In Old World monkeys it actually targets neurons...forget to use the link below to keep up with the latest in Addgene's viral vector services and resources...
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  Antibodies 101: Affinity TagsTypeBlog Post...bacteria, yeast, mammalian, etc.). To individually isolate a protein, there must be a special feature about...S-Transferase (GST) is a 26 kDa tag which can be isolated with glutathione resin. Like MBP, it can be removed...
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  An Addgene Co-op: The Intersection of Fun, Friendship, and WorkTypeBlog Post... data, and I was given a fantastic spreadsheet template to build out a report on. I was able to understand...cultivates trust between coworkers and that trust then translated into this incredible experience. Of course, ...
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  Year of the Camelid: Antibody StyleTypeBlog Post...cultural importance of these animals to human populations around the world. Here at Addgene, we love camelids...living cells or tissues. sdAbs can also be used to manipulate cellular functions, like in this early example...
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  CRISPR 101: Drug discovery and CRISPRTypeBlog Post...may be most effective. Once drug candidates are isolated, they can be validated for specificity to their...before time and money gets put into them in those later stages. Target identification Not all diseases ...
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  Plasmids 101: Gene, enhancer, and promoter trappingTypeBlog Post...selectable marker. Each insertion line can then be isolated and screened. Depending on the marker, traps can...require the trapping of endogenous enhancers to stimulate the expression of the reporter. The system is ...
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  Cellosaurus: A Cell Line Information DatabaseTypeBlog Post...LitSuggest Tool Following the “rabbit hole” of related cell lines when annotating a cell line Community...whether a cell line is known to be genetically manipulated, its anatomical origin, if cell line has ever...
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  Five Questions to Ask If You're Deciding Between a Master's or PhD ProgramTypeBlog Post... left four years later with a master’s. At the time my degree felt like a consolation prize, but in time...