Plasmids 101: Screens vs. Selections
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...worse yet have a screen that doesn’t yield any hits. Make sure you plan ahead and consider these questions...
We narrowed to 811 results for: mak
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Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog Post...elicit a strong immune response, factors that may make it especially suitable for in vivo research and ... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog Post...or for diagnostics, we suggest double checking to make sure your results will not be affected by methylation... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog Post...machinery to process gRNAs. Hijacking tRNA processing to make gRNAs In a recently published paper, Yinong Yang... -
Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur
TypeBlog Post... and devoted 100% of his energy and effort into making the startup self-sustainable. Within a year from... -
Transferable Skills Guide: Problem Solving
TypeBlog Post...other skills such as critical thinking, decision making, creativity, or data analysis. Conveniently, these... -
Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog Post...issues with some of the experimental designs used to make these measurements and hope this information can... -
Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog Post... the main source of IL-36 cytokines in the skin making it important to study what is happening within ... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog Post...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog Post...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you... -
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog Post... away. As a result, variable gene expression can make data hard to interpret or can interfere with the... -
Human Germline Editing Using CRISPR
TypeBlog Post...differences in editing between iPS cells and embryos make a case for conducting research in embryos should... -
Giving gRNAs a Facelift - Synthetic and Beyond
TypeBlog Post...modifications What is more stable than RNA? DNA! One way to make your gRNA more efficient on a budget is to simply... -
Transferable Skills Guide: Cross-team Communication
TypeBlog Post...We took steps towards solving this problem by 1) making sure we qualified these words when we discussed... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog Post...bioelectrical signals affect pattern memory and decision-making in somatic cell networks. “Developmental bioelectricity... -
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog Post...Be mindful of the restriction enzymes you chose. Make sure that enzymes that you are using are not blocked... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog Post... has revolutionized how we decode the genome by making it easy to create specific genetic perturbations... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog Post...deposited the library with Addgene because she wanted to make it as easy as possible for researchers to use this... -
Plasmids 101: Cre-lox
TypeBlog Post...types, during certain stages of development, or by making the Cre inducible (such as with tamoxifen or doxycycline... -
Working with Nuclear Receptors
TypeBlog Post...with a one-stop shop for plasmids and vectors, and making them available to all researchers for a reasonable...