Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,018 results for: ple

Showing: 701 - 720 of 1018 results

  1. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    ...cassettes for selection of transgenic plants. The complete GreenGate toolkit, now available at Addgene, also...tested GreenGate in Arabidopsis, it should in principle work in any plant species. Tricks of the trade...essential for scientific progress. Also, the more people use such a system, the more building blocks will...

  2. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    ...interviewing fellow Addgenies. I spoke with amazing people that were a part of the different departments of...felt like I could actually make connections with people. These were some of the things that really made...by Addgene. I have met some of the most genuine people who have shared amazing advice with me. Due to ...

  3. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    ...Science Communication group started. It's as simple as getting people together at a bar. Chapter 10: Maintaining...well as a platform for scientists to be viewed as people (#ActualLivingScientists). We also discuss the ...

  5. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...team costumes, and spooky science stories. Yes, please! At Addgene, we love finding ways to incorporate...bird feces, which get eaten by another snail, completing its lifecycle. Halloween genes The Halloween ...and drains all the blood out. Once the blood is depleted, the tongue atrophies and falls off. The louse...

  6. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ...affected by devastating diseases that affect far fewer people and therefore fail to garner much international...the Institute of Biosciences and Bioresources in Naples, Italy. His group works on spinal muscular atrophy...data from primary peritoneal mesothelioma tumor samples that they have used to identify candidate genes...

  8. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    ...YFP) to the new and improved fruit colors (e.g., apple, papaya, and tomato) and the photoactivatable fluors...quickly. Researchers should keep in mind that a very simple sequential or double restriction enzyme digest ...on the corresponding plasmid pages (e.g. see Supplemental Documents here). If you’re not sure where your...

  9. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ...observe what’s happening inside cells for at least a couple of decades. Green is the classic color, but fluorescent...signal-to-background ratio, thus, allowing higher resolution in samples containing substantial autofluorescence background...direction of biosensors capable of revealing, for example, how muscles work inside a living animal. For those...

  10. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR 101: Multiplex Expression of gRNAs and ...Cpf1: A New Tool for CRISPR Genome Editing and Multiplex Genome Editing with CRISPR-Cpf1 Day 4: Edit validation...

  11. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    ...kind of walking them through the steps of, for example, cutting open a heart to look at the inside. We...a lot of interactives and videos, as well, to supplement the hands-on [aspect of the labs],” says Caitlin...new technologies in education and ask ‘Can we implement that?’ [And think about] where we [eScience] are...

  12. Fostering communication in remote collaborations

    Type
    Blog Post
    ...helps improve your interpersonal relationships with people you’re collaborating with remotely. Depending on...as Slack. Share data and documents While having multiple scientists edit the same document may sound like...collaborators. Being able to present data from multiple aspects of a collaboration and immediately discuss...

  13. Oh, The Places You Can Go: Careers in Science Communication - Product Development

    Type
    Blog Post
    ...Iowa in the Department of Pharmacology, I taught a couple of lectures in the undergraduate pharmacology course... editing sphere.” But things aren’t always that simple, especially when your spouse is also in science... stumbled upon eScience by luck, by chance, by complete happenstance. It was on Indeed or one of the job...

  14. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...University of Akron, Ohio. Congratulations! There was plenty of geeky fun to be had at ASM Microbe as well as...science theme, the Artwalk on the exhibit floor had plenty of wonderfully nerdy art for sale, and photos of...presented at the conference, it looks like there are plenty more exciting plasmid tools on the horizon for ...

  15. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...skills help when it comes to finding a job. Many people in science will work in industry or take on collaborative...and their interest in science more generally to people outside of academia. Here are my recommendations... they start asking you questions. Remember that people like a good story and science involves characters...

  16. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    ...common lexicon for genome editing studies. These complementary goals are aimed at improving translation of ... loosely paraphrased in the tweet below. The complexity and challenges of homology directed repair (HDR...editing and low off-target editing rates across multiple systems. Read our introductory blog post, then...

  18. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...manipulated with mutations in the replicon. For example, the pMB1 ori maintains about 20 copies per cell... any questions? Need something clarified? If so please, post in the comments.  References Chatterjee A...Schultz PG. A versatile platform for single- and multiple-unnatural amino acid mutagenesis in Escherichia...

  19. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...an ITR during routine plasmid propagation, with complete ITR loss abolishing AAV packaging. To prevent ... like as long as it’s ~4.4 kb or smaller. Some examples include a reporter like GFP, some smaller CRISPR-Cas...also drive different levels of expression. For example, CAG is a strong promoter that drives very high...

  20. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...jGCaMP7 sensors with different properties (for example, overall brightness or on/off kinetics) that makes...quantify targeting of particular cell types. For example, the mDlx enhancer restricts expression to GABAergic... particular catalog item is available for order please email [email protected] with the vector name and...
Showing: 701 - 720 of 1018 results