We narrowed to 1,010 results for: RING;

1. Antibodies 101: The Basics of Western Blotting

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   ...Westerns are divided into two categories, native and denaturing. In a native western, the protein’s secondary...is separated through a matrix by charge. In a denaturing western, the protein is denatured to its primary... purpose of this blog, we will be focusing on denaturing westerns.  Separating a protein mix by size Sodium...The wet transfer process is very effective in transferring a broad range of protein sizes but takes hours...blocking the membrane  from binding to the antibodies during the stain. The ideal blocking buffer depends on...loading control when assessing protein abundance or comparing samples across lanes.  For a typical loading control...

2. Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies

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   ...social media, you could be sharing your research or finding opportunities to bring science to the community...the same page with the potential PI in terms of mentoring style and expectations. Make sure you will enjoy...mantra and never forgot it. Usually when I see inspiring artists who are thinking about getting into the... the process down into steps and set aside time during the week to tackle each one.  Get the toughest ...

3. IDP and your PI: A Roadmap for Career Planning and Personal Development

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   ...year, graduate students and post-docs alike are wondering where the time has gone. Are we any closer to ...to graduating, publishing that key paper, or figuring out a career path? Many trainees are developing Individual...accomplishments, from learning new techniques to finally figuring out a tough experiment, sets a positive and encouraging...Good Manager Anyway Developing Transferable Skills During Science Training ... Mary Gearing...

4. Tag Your Favorite Yeast Genes with Ease

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   .... pombe. The protocol is surprisingly simple, requiring only a PCR product containing the modifying sequence...affinity purification of protein complexes. John Pringle and Jürg Bähler have deposited a large collection...genome modification in yeast developed by Dr. Pringle’s former lab at UNC Chapel Hill. Bähler et al.(2...McKenzie A 3rd, Steever AB, Wach A, Philippsen P, Pringle JR. Yeast. 1998 Jul;14(10):943-51. Longtine MS,... DJ, Shah NG, Wach A, Brachat A, Philippsen P, Pringle JR. Yeast. 1998 Jul;14(10):953-61. Note: Marcy...

5. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

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   ...openness and sharing in science.  We’re excited to support the neuroscience community by sharing not only ...capsid proteins that determine the AAV serotype. During transduction, the viral capsid proteins bind to...enhancing anterograde transport efficiency, and engineering viral capsids that target specific cell types...science, we aim to accelerate the rate by which sharing speeds science.   Check out the precise neuronal...

6. 2023 and 2024: Reflections and Looking Ahead

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   ...believing, as we do, that sharing speeds science.  This year, Chonnettia will be sharing more of her perspective...what we accomplished in 2023 and thrilled to be sharing our plans for 2024.  In 2023, Addgene distributed... of innovation and impact made possible by the sharing of tools for research: discovered and developed...you a healthy, productive, and thriving 2024, mirroring our own aspirations for Addgene. On behalf of ...

7. Mapping the 4D nucleome with CRISPR/Cas9

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   ...new technique, developed in Thoru Pederson’s lab, brings us one step closer to mapping the 4D nucleome, ...with the previously established physical map. In comparing pairs of targets ~2 Mbp apart, they noticed that...may be imaged under lower illumination settings, lowering photobleaching and phototoxicity concerns and ... of chromosomal translocations or chromosomal shattering (chromothripsis). As this study joins the pantheon... Mary Gearing...

8. RNA Interference in Plant Biology: New Tools for an Old Favorite

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   ...DCL) process the dsRNA into specific types of interfering RNAs.   RNAi vs. CRISPR: Complementary techniques...fundamental steps: creation of the RNAi trigger delivering the RNAi trigger characterizing the RNAi phenotype...from a long hpRNA trigger, only a single ~21-nt interfering RNA is produced using shRNA/amiRNA. Although ...evaluated for their gene silencing efficacy, thereby requiring a substantial time investment. Once the target...widely implemented in plants, such as in the model flowering plant Arabidopsis thaliana (Li et al., 2013; Zhang...Gong B-Q, Guo Y, Xu L, Zhang X-N, Li J-F (2018) Engineering Artificial MicroRNAs for Multiplex Gene Silencing...

9. Embracing Serendipity: A Crucial Element in the PhD Journey

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   ... the importance of leaving room for serendipity during your PhD studies and offer two simple steps to ...you to discussions beyond your immediate field, fostering a diverse range of perspectives and sparking innovative...capture fleeting thoughts and connections that emerge during moments of inspiration. Additionally, journaling...enrich the depth and creativity of your work, while offering mental health benefits by encouraging activities...How to Lead a Great Meeting Form Your Own Peer Mentoring Group...

10. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

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    ...thought leader in scientific sharing, leading by example to increase sharing in the community Addgene fosters...organization what do you think of? Maybe the term brings to mind a social service organization like the ...believes there is strength in diversity and in empowering team members to take initiative and give their...our mission to accelerate research with easier sharing and collaboration. Joanne and all the Addgenies...