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...needs to be added to media after autoclaving. If spread on top of pre-made plates, make sure it is evenly...Although blue-white screening is probably the most widespread way to select for plasmids containing an insert...

...transient gene expression in a particular cell type to CRISPR genome editing, optogenetics, and chemogenetics...different ages." Retinal Degenerative Diseases. Springer International Publishing, 2016. 501-507. PubMed...

...Gearing Listen to the TRIM-Away podcast segment CRISPR and RNAi have helped researchers alter DNA sequence...Segment Jerry Crabtree’s lab has developed a new CRISPR/Cas9-based system to study epigenetic modulation...

...happening within the keratinocytes. “We hope to use CRISPR libraries from Addgene to set up assays to see ... contraction defects. The lab also plans to use CRISPR plasmids to generate deletions of candidate genes...

... 96 parts including terminators and promoters, CRISPRi parts, selectable markers, transformation acceptor...promoters, and inhibited gene expression using CRISPRi. The kit functions in the model cyanobacterium ...

...Split-BioID plasmids Find CRISPR plasmids for labeling genomic regions Find CRISPR plasmids for purifying...

...or preparation, and deliver CRISPR components for gene disruption or CRISPRi. While designed to study the...

...practices could be an actionable way to tackle widespread irreproducibility. Luckily, there were other ...practices could be an actionable way to address the widespread issue of irreproducibility. Use your network...

...our “google doc of gratitude.” We must keep spreading the word about materials sharing, but we don’t... who support us by depositing, requesting and spreading the word. Our blog and site resources are just...

... position the tips indepently making it easy to spread out your samples. This can be useful if you’re ...plates onto agar q-trays where the samples must be spread much farther apart before plating. With this fine...