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Showing: 721 - 740 of 968 results
  1. Sleeping Beauty Awakens for Genome Engineering

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    Blog Post
    ...use of viruses is also important for social and regulatory reasons. 1.) The transposon consists of a mirrored...your gene-of-interest, either constitutively or regulated by doxycycline. The Sleeping Beauty transposase...
  2. Choosing Your Perfect Empty Backbone

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    Blog Post
    ...backbones) are frequently used in molecular biology to isolate, multiply, or express the insert they carry in ... system is great for overexpressing YGOI in an isolated, controlled environment. In most cases bacterial...
  3. Summer SciComm: Science Storytelling

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    Blog Post
    ...even by introducing a person that the audience can relate to. It doesn’t need to be anything fancy; it just... anthropomorphized animals, or even seemingly unrelated stories that they then tuck the science into. ...
  4. New Viral Vectors - Winter 2025

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    Blog Post
    ...viral vector repository with a nice cup of hot chocolate is absolutely delightful. Grab a hot beverage ...forget to use the link below to keep up with the latest in Addgene's viral vector services and resources...
  5. Science Careers: Unruly Interests Feed Many Paths

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    Blog Post
    ... projects studying developmentally programmed regulation of DNA replication and gene transcription during... useful and productive, forming a different constellation of interests and skills than what fits for applied...
  6. Viral Vectors 101: What is a Virus (and a Viral Vector)?

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    Blog Post
    ...genomes can be transcribed into mRNA, directly translated into proteins, or, in the case of retroviruses...which is then transcribed into mRNA before being translated into proteins. RNA viruses replicate their genomes...
  7. Don’t Wait To Launch Your Health Science Startup

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    Blog Post
    ...companies.  The majority of these meetings acted as a platform for significant company advancement. Be relentless... you’re able to answer every possible question related to it. First impressions are everything and you...
  8. Celebrate National Book Month with Addgene

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    Blog Post
    ...Sacks "Neurologist Oliver Sacks examines the relationship between the human brain and music: how we perceive...roles microbes play in our ecosystem, examining relationships between microbes and insects, mammals, and plants...
  9. New and Upcoming Viral Vectors - June 2019

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    Blog Post
    ...provides improved detection for tracking large populations of neurons in wide-field imaging (Dana et al.... intersectional control of specific neuronal populations. We now offer a Flp-expressing AAV, as well as...
  10. Summer SciComm Series: A PhD in Science Communication

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    Blog Post
    ...the same myths and misconceptions fifteen years later. What does that look like?  One of my more recent...designs could work to slow the spread of illness. I later helped run workshops where I showed people how they...
  11. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

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    Blog Post
    ...trigger other forms of neuronal signaling and regulation. To create iGluSnFR, the Looger lab wedged GFP...soluble protein CAG: Generic strong promoter. Formulated as AAV-DJ for neuronal expression. CAG-FLEX: ...
  12. Pushing the Limits of DNA Assembly

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    Blog Post
    ...DNA – perfect for transforma­­­tion or as a PCR template. Some of the applications of the NEBuilder HiFi...plasmids can be directly transformed into E.coli for plating, screening and generation of sgRNA. Generation ...
  13. Four Factors that Differentiate the Stem Cell Field

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    Blog Post
    ...that stem cell scientists are so focused on translational research is encouraging. Patients are key to...depositors Marius Wernig, Kristin Baldwin, Kathrin Plath, Hans Schöler, and closing keynote speaker Rudolph...
  14. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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    Blog Post
    ...process in detail often suggests new strategies to manipulate that process. We began our research with a simple...approaches. Moreover, the cost of reagents is relatively modest, requiring expressed Cas9 protein, transcribed...
Showing: 721 - 740 of 968 results