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  2. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...proteins are also involved in the transcriptional regulation of the AAV p40 promoter which drives Cap expression...promoter. Alternative splicing and differing translational start sites generate the three VP proteins. ...

  3. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...bacteria, yeast, mammalian, etc.). To individually isolate a protein, there must be a special feature about...S-Transferase (GST) is a 26 kDa tag which can be isolated with glutathione resin. Like MBP, it can be removed...

  6. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...confidentiality where it is appropriate. This grows our relationship with our community members. We also find that...Mission Meet the Addgene Leadership Team Get the Latest News from Addgene ...

  7. Sleeping Beauty Awakens for Genome Engineering

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    Blog Post
    ...use of viruses is also important for social and regulatory reasons. 1.) The transposon consists of a mirrored...your gene-of-interest, either constitutively or regulated by doxycycline. The Sleeping Beauty transposase...

  8. Choosing Your Perfect Empty Backbone

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    Blog Post
    ...backbones) are frequently used in molecular biology to isolate, multiply, or express the insert they carry in ... system is great for overexpressing YGOI in an isolated, controlled environment. In most cases bacterial...

  11. Summer SciComm: Science Storytelling

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    Blog Post
    ...even by introducing a person that the audience can relate to. It doesn’t need to be anything fancy; it just... anthropomorphized animals, or even seemingly unrelated stories that they then tuck the science into. ...

  13. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...genomes can be transcribed into mRNA, directly translated into proteins, or, in the case of retroviruses...which is then transcribed into mRNA before being translated into proteins. RNA viruses replicate their genomes...

  14. New Viral Vectors - Winter 2025

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    Blog Post
    ...viral vector repository with a nice cup of hot chocolate is absolutely delightful. Grab a hot beverage ...forget to use the link below to keep up with the latest in Addgene's viral vector services and resources...

  15. Science Careers: Unruly Interests Feed Many Paths

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    Blog Post
    ... projects studying developmentally programmed regulation of DNA replication and gene transcription during... useful and productive, forming a different constellation of interests and skills than what fits for applied...

  18. Don’t Wait To Launch Your Health Science Startup

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    Blog Post
    ...companies.  The majority of these meetings acted as a platform for significant company advancement. Be relentless... you’re able to answer every possible question related to it. First impressions are everything and you...

  19. Celebrate National Book Month with Addgene

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    Blog Post
    ...Sacks "Neurologist Oliver Sacks examines the relationship between the human brain and music: how we perceive...roles microbes play in our ecosystem, examining relationships between microbes and insects, mammals, and plants...

  20. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...provides improved detection for tracking large populations of neurons in wide-field imaging (Dana et al.... intersectional control of specific neuronal populations. We now offer a Flp-expressing AAV, as well as...
Showing: 721 - 740 of 970 results