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We narrowed to 1,085 results for: ANT;

Showing: 721 - 740 of 1085 results

  1. Newly Updated AAV Data Hub!

    Type
    Blog Post
    ...can save you significant time and money. For instance, this report shows that anterograde, monosynaptic...see from the community is your negative data. We want to make sure people are selecting AAVs that will...work was cited by others?)  Ready to submit?  Fantastic! The next 25 submissions will receive a 20% off...

  2. Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up

    Type
    Blog Post
    ...Instrumental Access participants. Like Seeding Labs, Addgene also feels it’s incredibly important for scientific...challenges. However, these same researchers face significant barriers to scientific discovery and career development...accelerate biology research in the labs they’re granted to, as well as shine a light on some of the great...

  3. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    ...identify what your plasmid contains and other important information. Tip: A lowercase "p" is often used...simple piece of information can often convey many important details. Once you know the backbone a plasmid ... on, you can usually derive: a) the bacterial antibiotic resistance, b) the promoter that drives the insert...position 295 was mutated to an Alanine, Q295A.  Mutant version: pBACKBONE-Flag-hGene(Q295A)-EGFP  Wildtype...

  4. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ... do not express Cre or Flp. Here at Addgene we quantify the amount of transgene recombination for every...fibrillary acidic protein (GFAP) for astrocytes it is important to test it in the brain region of interest, especially...Zolotukhin S, Reier PJ, Mandel RJ, Muzyczka N (2004) Recombinant AAV Viral Vectors Pseudotyped with Viral Capsids... Analysis Platform to Assess the Identity of Recombinant Adeno-Associated Viral Preparations from Viral... the Addgene blog Plasmids 101: FLEx Vectors Important Considerations When Using AAVs Three Key Considerations...

  5. A Scientist's Guide to Ebola

    Type
    Blog Post
    ...component of vaccines and the target of neutralizing antibodies and inhibitors of attachment and fusion. Recommended... Genome Portal at the University of California, Santa Cruz, offers sequence data on five strains of Ebola...outbreak and earlier ones, along with links to the relevant scientific literature.  -In light of the current...Medicine have made their complete collection of relevant papers freely available here. -PLoS and The New...

  6. What's New in CRISPR - May 2019

    Type
    Blog Post
    ...libraries also include 2,400 gRNAs without any significant hit across the E. coli genome as a negative control...PCV and PCV-Cas9 plasmids Selective curbing of unwanted RNA editing (SECURE) base editors Keith Joung’... in human cells. Thus, they engineered two CBE variants with mutations in the rat APOBEC1 that decreases... fold and >3,800 fold in human cells with no significant reduction in editing efficiency. Read the paper...

  7. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...typically grown in liquid culture without antibiotics to give the antibiotic resistance gene time to be expressed...being performed at Addgene, this adds up to a significant time savings. You can also save yourself time...homemade competent cells to ensure good efficiency.  Want even more tips for transforming your cells? Check...

  9. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...Protocols Recombinant Antibody Affinity Purification with Protein A or Protein G Recombinant Antibody Affinity...like... Antibody Plasmid Collection Antibody Blog Posts Antibody Guide Transfection for Recombinant Antibodies...describes how to affinity purify recombinant antibodies from cell culture supernatant using Protein A or Protein...to affinity purify recombinant antibodies. Workflow Timeline Day 1: Purify antibody Day 2 or later: Buffer...Equilibrate filtered tissue culture supernatant containing the recombinant antibody to room temperature (see Transfection... will ensure depletion of the recombinant antibody from the supernatant. Wash the column with 25 mL of...

  10. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    ...a request and order if approved.  I want to stay updated!  We want to keep you updated! Subscribe to the...will be available for request in three different quantities: 10x 100 µL (1 mL), 25x 100 µL (2.5 mL), or 50x...process. This process will take 1–2 days, and we anticipate approving the vast majority of requests.  If ...

  11. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...ability to label live cells make this system truly advantageous. This new technique, developed in Thoru Pederson... other orthologs. Ma et al. tested their dCas9 variants using gRNAs specific for telomeric sequences and... live cell imaging of genomic loci. Ma et al. anticipate that the technique will be applicable to studies...shattering (chromothripsis). As this study joins the pantheon of useful CRISPR/Cas9 techniques, we at Addgene...

  12. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ... be able to store your sample without having substantial degradation and which experiments you can use...preventing the activity of DNase. While this is very important to preserve the stability of your DNA, it will...elution of plasmid DNA through their kit. The advantage of using a Tris buffer is that it allows better...that elution buffer onto your DNA. Knowing how you want to use the plasmid DNA can help you choose the best...

  13. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...is reflected in the biology, too. CRISPR is an elegant bacterial immune system and an efficient gene editing...sequence. The spacer sequence is the RNA guide, like a wanted poster that Cas9 uses to track down its suspect—the... repeat section of the crRNA, forming the repeat:antirepeat duplex. tracrRNAs also contain multiple stem...have a blog post on the structural biology of Cas9. Want a simpler guide to keep at your desk? We’ve got ...

  14. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    ...that had never been done at Addgene before. This meant I was responsible for establishing a new report ...certification on social media data, and I was given a fantastic spreadsheet template to build out a report on....more people who need it!  After a few months, we wanted to share these, and other, findings with the larger...to-dos, I also found time to go out to sushi restaurants for lunch with coworkers and visit the office...

  15. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ... effective tools but neglect some biologically relevant situations. Tissue-specific genes – the genes...its functions can be observed. Functionally redundant genes – multiple genes which function in overlapping...to generate a collection of monitored genes and mutants - viral integration, transposon, and many more!...10.7554/eLife.67667 Springer, S. P. Gene Traps. Plant Cell. (2000) 12(7): 1007-1020. DOI: 10.1105/tpc.12.7.1007...

  16. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...Genome Engineering and CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR 101: Multiplex Expression...CRISPR is an incredibly fast-moving field, and we want to make it easy for you to keep up with new developments...editing plasmids for mammalian systems, bacteria, plants, and yeast. Popular cytosine base editor pCMV-BE3...

  17. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    ...or personnel to constantly prepare them for distribution. One of the major advantages to depositing materials...depositor’s dedication to scientific sharing, we wanted to celebrate our depositing PI’s who have the most...Given the increasing popularity of CRISPR in the plant community, this has saved us hours of work responding...

  18. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    ... drudgery to some scientists, MTAs are important—they guarantee certain rights and protections for depositor...another blog post: Why Do I Need an MTA Anyway?) Don't want to wait? Talk with your TTO Has a pending legal ...request MTAs and deposit agreements to basically instantaneous in many cases.  Suggest that they get an Addgene...

  19. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    ... discipline and reduces the dissemination of unwarranted claims or unacceptable interpretations. A good...journal.  As a postdoctoral researcher studying plant development and abiotic stress tolerance, I have...peer-reviewer you wish for The last thing that I wanted to mention about the peer-review, is that as a ...ratio in Arabidopsis, (2) expression patterns in plants with enhanced sodium accumulation in their roots...

  20. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...updated Nov 10, 2020. Now that we know all about antibiotic resistance genes, let’s consider another basic...one plasmid type in your cells at a time? Do you want to make a lot of your plasmid? Is the gene toxic...choice of ori depends on how many plasmid copies you want to maintain, which host or hosts you intend to use...amount of aeration, temperature, culture volume, antibiotic, and medium can all affect copy number. Some ...
Showing: 721 - 740 of 1085 results