... Sometimes it’s a good way to find out what’s happening in the office next to you. There is an active ...sometimes stupid or uncomfortable things that have happened to them. To have that conversation out in the ...

...emission is green or red light. For example, T-Sapphire, LSSmOrange, and LSSmKate. Fluorescent Sensors.... al. (2010) “Fluorescent proteins and their applications in imaging living cells and tissues”  Physiol...

...the basics of how to weigh reagents. Wear the appropriate personal protective equipment (PPE) prior to ...contain the reagent while on the balance, and an appropriately sized container to put the reagent in. For some...container. Dispose of the excess material into an appropriate waste container if the material is biohazardous...biohazard solid waste container. Conclusion Appropriately weighing reagents is important to ensure the...

...101: Types of Viruses Viral Vectors 101: Viral Applications Resources on addgene.org Addgene's Lentiviral...Liu, H., Xiao, H., Conway, J. A., Hunter, E., & Kappes, J. C. (1997). Functional RT and IN incorporated...

...and retroviral expression system for using this approach in in vivo studies (Fujita et al., 2018). In addition...DUX4, a transcriptional regulator that when inappropriately expressed can cause facioscapulohumeral muscular...

...labels for fixed-cell imaging, not for live-cell applications. Thus, these fluorophores are often bulky and...budget gets a bit thin, small-molecule labeling approaches can provide a fresh infusion of photons for your...

... tube contains information about that tube’s appropriate location. The barcode also identifies the contents... sample tubes that we use match those of the appropriate deposit. Using barcoded tubes is part of Addgene...

...working in the lab. Do not eat, drink, chew gum, or apply makeup in the laboratory. Before you start your ...and uncluttered. Disinfect your workspace with appropriate materials. For example, you may use 70% ethanol...your lab supervisor what is available and the appropriate contact times for each agent. Make sure you have...autoclaved. Conclusion Although simple, following appropriate BSL-1 and BSL-2 protocol goes a long way. It ...

...similar approach would be good for your work. Hopefully this blog helps you with that…and happy flowing...

...particles can be identified after negative staining and appear darker than full vector particles.     ...replaced with a new vial of early passage cells. Approximately two weeks post-thaw, cell culture supernatant...

...easiest to train individuals who are polite, appreciative, and clear about their needs. Remember, your...trainees one day when you are the senior personnel! Happy training! Resources Resources on the Addgene blog...

...papers and then feel ready to write. What actually happened was that each paper taught me a few things and...to read every single paper. I also learned to appreciate the discussion sections of the primary literature...

...sequences into multiple vector systems. With the appropriate entry and destination vectors, one can use Gateway...elements to express the gene of interest in the appropriate system Recombines with the entry clone to generate...

...fluorescent proteins (FP) have also been important in mapping, monitoring, and manipulating neuronal circuits...combining advantages of FPs and epitope tags, smFPs appear to be very effective probes for finely visualising...

...workflow - analyzing the data - is often under-appreciated or over-looked.  Synthego has developed a new...analysis Once the analysis is complete, a new screen appears with a graphical representation of the results ...

...Motif (PAM), the sequence NGG.  If these conditions apply to your experimental target, SpCas9 and its enhanced...specificity over a single SpCas9. The drawback of this approach is that two sgRNAs targeting opposite strands ...

... have created a yeast-based directed evolution approach with some notable improvements. Directed evolution...specific guide RNA (gRNA) sequences. But these approaches rely on continual expression of both dCas9 and...

...transduced by PHP.B. The Deverman Lab took a broad approach and searched for genetic difference between the...phenotype in mice. They took a classic genetics approach and interbred C57BL/6J and BALB/cJ mice to map...

... providing support "for development of tools, approaches, and infrastructure necessary for direct tests...recent). They have diverse traits with good genetic mapping morphology and interesting behaviors. There is ...

...possibilities for the clinical and therapeutic applications of iPSCs. The generation of human iPSCs (hiPSCs...., Recent technological updates and clinical applications of induced pluripotent stem cells. Korean J ...