We narrowed to 1,006 results for: CAL;

...., 2021). Another version of the spike protein, called HexoPro, was developed by Jason McLellan’s lab....genome-wide CRISPR libraries, Brunello (knock out) and Calabrese (activation), from Addgene to better understand...bidirectional CRISPR screens identify mucins as critical host factors modulating SARS-CoV-2 infection. ...

...bacteria through the mail due to laws concerning genetically modified organisms. Since Addgene is very proud...individually. Enter the BIOROBOT! This robot is called the Qiagen BIOROBOT Universal Platform and it suits... for a mini prep, except it does it on a larger scale. Using the BIOROBOT Universal Platform is ideal ...

... to be re-engineered for each gene, making wide-scale gene activation seem next to impossible. The development...methods greatly improved CRISPRa, expanding its practicality and popularity in research (Tanenbaum et al....JS, Nishimasu H, Nureki O, Zhang F (2014) Genome-scale transcriptional activation by an engineered CRISPR-Cas9...

...Addgene’s Lab Manager, was taking classes at her local community college when she took her first job at... and hoping to pursue. The choice to not go to medical school was devastating. At the same time, she has...circumstance. She was working as a research and technical assistant, when her life changed––she had a daughter...

...unintelligible to a lay audience. My lay paragraph typically includes a couple of sentences of introduction... rejected on some occasions. Strategize, choose calls that suit your career stage / research area, and...peers and collaborators can also help you get some critical insight into what you're doing wrong. All this...

...remarkable progress on establishing a system for the chemical replication of RNA in the absence of evolved enzymatic... about using CRISPR/cas to address the growing clinical problem of antibiotic resistant bacteria. See ... and Sun Yat-sen University presented two new technical developments. Using the Tet-on system, his lab...

...edits or knock-ins. Editing efficiency decreases drastically once your cut site is more than 10 bp away from... mutagenesis plasmid (MP6) permits quick, large-scale mutation of the gene of interest. They created a... a selection phage (SP) to carry catalytically dead SpCas9 (dCas9) fused to the bacterial polymerase subunit...

...but close together in terms of where they are physically located. DNA is then sheared into pieces small...on-target enrichment. 2. Determining the spatial and hierarchical organization of chromatin interaction networks...CAPTURE 2.0 is the construction of spatial and hierarchical chromatin interaction networks. Think of these...

...sequencing primers for quality control. At such a scale, automation is crucial, both for speeding things...to the same principles listed here. It employs biological best practices. For example, it ranks guides ...published on how to do it best. Scientists are typically advised to mutate the PAM to which the guide binds...

...bicistronic vectors take advantage of an element called an Internal Ribosome Entry Site (IRES) to allow...use the 2A peptides instead of an IRES when stoichiometrically equivalent levels of expression are required...journal.pone.0018556. Epub 2011 Apr 29.) PubMed. 5. Scalable signaling mediated by T cell antigen receptor-...

...-55 °C for the homologous region (calculated using Oligo Calculator). Insertions - To insert short sequences...parental DNA (37 °C for 15 mins). This enzyme specifically cuts methylated DNA, so it will selectively ...

...Liang et al. 2017).  PEG-mediated methods, while technically less difficult to perform, often show low efficiency...ribonucleoprotein electroporation of zygotes." Journal of Biological Chemistry (2016): jbc-M116. PubMed PMID: 27151215...Cell-Type-Specific Gene Editing." Journal of the American Chemical Society 140.21 (2018): 6596-6603. PubMed PMID:...

...culprit for these unusual cases was either 1) a physiological effect of SARS-CoV-2 toward destabilizing one...Adenovirus (and/or to its partner, AAV) or 2) a sociological effect of the COVID-19 lockdowns causing a lack...Adenoviruses: General Features. In Reference Module in Biomedical Sciences. Elsevier. https://doi.org/10.1016/B978...

...not the case for knock-ins. Knock-in mutations typically require an exact DNA sequence to be incorporated...know that HR is often mutated in cancer cells, specifically in breast and ovarian lines commonly used in...common editing pathway for DNA repair. To tilt the scales in your favor for an HDR edit, you can try one ...

...methods avoid many of the drawbacks of kits and typically follow the same principles: cells are lysed, RNAs...method described in Kim and Morrison is nearly identical to that of commercially available kits but instead...B., & Gillespie, D.A. (1979). Preparative and analytical purification of DNA from agarose. Proceedings...

... familial aspect of Addgene. As our size has drastically increased, it would have been a tight squeeze...event! Addgene company culture At Addgene we organically developed the philosophy of  “work hard, play...department) right in your breakroom. 5. Throw some physical activity into the mix It’s easy to sit around ...

...community to help Involve family. If you don’t have local family to help, create “fake” family by forming ...help you do your job better. #8 Thou shalt enthusiastically foster independence Your partner can make ... fold their laundry, take public transportation, call a cab and remember their own school assignments,...

...uses a “nicking” Cas9, in which one of Cas9’s two critical enzymatic residues has been converted to an alanine...deaminases edit A-to-G.) In short, the impaired Cas9 localizes the deaminase to where the edit is desired, and...to dCas9.  This technique has been exploited to localize factors such as transcriptional activators and...

...generate a dot plot (Figure 1). Clustered events are called a population.                             ...of markers you use to define your population is called your “gating strategy.” This series of markers ...around clusters to define populations. This shape is called a ‘gate’; it tells the machine that everything ...

... relative to empty particles (black arrowhead). Scale bar = 100 nm.   AAV resources Browse AAV preps... a concentration step prior to being frozen. Specifically, lentiviral particles are collected from the... strain of E. coli. In addition, plasmids are typically prepared using endotoxin-free plasmid purification...