We narrowed to 824 results for: EED

1. Hot Plasmids and Viral Preps - May 2021

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   ... easier for you to find and use the plasmids you need. Here's what you'll find in this post: Pooled library...

2. 3D Printing Meets CRISPR Cas9

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   ...compelling, and I encourage you to hold one. Why do we need physical models of protein structure? Structural...

3. PITChing MMEJ as an Alternative Route for Gene Editing

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   ...created in about a month and a half, without the need for complicated cloning of homology arms. MMEJ...

4. AAVs CREATed for Gene Delivery to the CNS and PNS

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   ...transgene into a specific cell type in the brain, you needed to make a transgenic mouse. Combining AAv.PHP.eB...

5. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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   ... and stability of the Cas9 protein in the host. Indeed, research has shown that a Cas9 protein from Geobacillus...

6. AAVs in Retinal Gene Therapy

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   ... systemic delivery of high doses of vectors are needed. Additionally, AAVs do not integrate in the host...

7. Plasmids 101: Secondary Nanobody Toolbox

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   ...Bacteria: Table 2 also has a link to the plasmid you’ll need to express your nanobody of choice in bacteria. ...

8. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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   ...Flexible GS linkers may also work. You will also need to generate negative control constructs — more on...

9. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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   ...promoter sequence. Thus, T7 RNA polymerase also needs to be present in the cells to transcribe the viral...

10. The Importance of a Fun Workplace: Company Culture at Addgene

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    ...sounds a little neurotic, but hey- sometimes fun needs to be had in an orderly and timely fashion. 9. ...

11. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    ...this versatile protein to suit their experimental needs, and some of the common applications in the lab....

12. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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    ...RNAs) or studying RNA processing in live cells. Indeed, groups have utilized dCas13 fusion proteins for...

13. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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    ...arise and tools to deactivate specific genes are needed. Targeted mutation has been demonstrated in zebrafish...

14. New Tools Enable CRISPRa for Neuroscience Applications

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    ...laborious because multiple plasmids/viruses are needed to deliver all the protein and RNA components, ...

15. Rett Syndrome: A History of Research and Therapeutic Outlooks

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    ...researchers can find the reagents and resources they need to cure Rett Syndrome.  If you’re new to Rett Syndrome...

16. To Codon Optimize or Not: That is the Question

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    ... a protein in its native host there is likely no need to codon optimize as this gene should already be...

17. Overcoming the AAV Size Limitation for CRISPR Delivery

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    ... for some applications, it doesn’t eliminate the need to deliver larger cargo such as base editors and...

18. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    ...requirement for Cas9-mediated DNA cleavage. The need for more PAM sequences While PAM sequences for the...

19. Finding nucleic acids with SHERLOCK and DETECTR

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    ...sensitivity and specificity without compromising speed, ease of use, and portability. This method can be...

20. Plasmids 101: Plasmid Incompatibility

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    ...replication and the replication control machinery (Need a refresher on the Origin of Replication? See our...