We narrowed to 807 results for: Eng;

1. Educational Planarian Tools for Teaching Developmental Biology Techniques

   Type

   Blog Post

   ...experiments are either not accessible or too challenging for amateur scientists. Planarians, wormy creatures...

2. 6 Tips for Grant Writing

   Type

   Blog Post

   ...approach that problem in a novel way. Finally, engineering new applications to solve problems will provide...

3. Adapting Toehold Switches to Detect Zika Virus

   Type

   Blog Post

   ...2014, as postdoctoral fellows with Jim Collins and Peng Yin at the Wyss Institute (Harvard University), ...

4. Viral Vectors 101: Chemogenetics

   Type

   Blog Post

   ...Chemogenetics relies on genetically introducing engineered receptors that are only activated by otherwise...

5. Antibodies 101: Choosing the Right Isotype

   Type

   Blog Post

   ...secondary antibody binding and therefore the signal strength (Fig. 1). Monoclonal primaries If you do decide...

6. Antibodies 101: Stripping and Reprobing Western Blots

   Type

   Blog Post

   ...time you strip a blot, you should not compare the strength of bands from different rounds of detection. Finally...

7. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

   Type

   Blog Post

   ...DNA Extracts. Pooled libraries are a bit more challenging to QC by NGS as they are often quite different...

8. Tips to Make the Most of a Scientific Conference

   Type

   Blog Post

   .... This made sure I didn’t zone out and kept me engaged throughout the day. After the conference If there...

9. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

   Type

   Blog Post

   ...access to routinely, and it can be especially challenging when trying to develop antibodies against unstable...

10. 10 Basic tips for mammalian cell culture

    Type

    Blog Post

    ...Additional Resources on the Addgene Blog The Challenges of Cell Culture Cloning Mammalian Cells with ...

11. 3D Printing Meets CRISPR Cas9

    Type

    Blog Post

    ...colleagues to reduce off-target hits in a rationally engineered Cas9 with improved specificity. Hold this flexible...

12. Cloning Mammalian Cells with the Agarose Method

    Type

    Blog Post

    ...Time CRISPR User (by a 1st Time CRISPR User) The Challenges of Cell Culture Plasmids 101: Mammalian Vectors...

13. Plasmids 101: Optimizing Plasmid Yields

    Type

    Blog Post

    ...of endA+ strains Strains like Stbl3 have been engineered to reduce plasmid recombination, but are also...

14. Experimenting with New Careers while in Grad School

    Type

    Blog Post

    ...realized I wanted a career in science outreach: engaging the public to promote an awareness and understanding...

15. Polymerase Chain Reaction Overview and Applications

    Type

    Blog Post

    ...standard curve, which can be time consuming and challenging to get right. ddPCR uses a water-oil emulsion...

16. Hot Plasmids and Viral Preps - May 2021

    Type

    Blog Post

    ...(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE from Hongkui Zeng's lab now available as a ready-to-use viral prep ...

17. Hot Plasmids: Winter 2025

    Type

    Blog Post

    ... developed in human cells, has been uniquely challenging to apply in dicotyledonous plants, or dicots....

18. Common Injection Routes in Mice

    Type

    Blog Post

    ...Organisms Additional resources on Addgene.org Genome Engineering Guide CRISPR Mammalian Expression plasmids Cre-Lox...

19. Viral Vectors 101: Producing Your rAAV

    Type

    Blog Post

    ...Ceballos-Diaz, C., Liu, X., Paterno, G., Mejia, C., Deng, D., Menezes, K., Londono, L., Arjona, K., Parianos...

20. Twenty Years of Addgene Sharing: CRISPR

    Type

    Blog Post

    ... the easy part; using them can be a bit more challenging. Addgene’s free educational resources help scientists...