We narrowed to 1,082 results for: IND

...ssDNA molecules in vitro. Figure 1: Cas9 binding to the cleaved DNA duplex. Image from Richardson...diseases in which sequence replacement holds some kind of fitness advantage but error-prone repair of the...on Your HDR Basics Learn How to Design Your gRNA Find the Ideal CRISPR Software for Your Needs Resources... on Your CRISPR Background with Our CRISPR Guide Find CRISPR Plasmids for Your Research Check out Our ...

... Podcast.       Resources at Addgene Find CRISPR resources Find CRISPR pooled libraries Learn about other...editing and screening) such that thousands of individual edits can be studied quickly. Involvement with.... the nonprofit, sharing ethic that Addgene has behind it is something that very much resonates with me...

...You’ve committed to the daunting task of finding new factors in your biological system of interest, the...are assessed, regardless of whether they enhance, hinder, or have no effect on the process you are interested...everything else dies off. For a screen, you would individually treat cells with many different drugs and then... forward screens. A given panel of drugs are individually administered and then outcomes are measured....

...necessary for most ChIP experiences, but the increased binding can mask the epitopes antibodies recognize. It ...In the case of histone modifying proteins, which bind quite strongly to DNA, crosslinking may not be necessary...to the DNA, depending on how tightly the proteins bind natively. But the amount of cross-linking can affect...Introduction to Antibodies Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene...

...the Fc portion of different antibody isotypes. Binding to complement system proteins, which can initiate...chain domain as your primary antibody but doesn’t bind your antigen of interest. Isotype controls are used...staining you see is due to target-specific antibody binding rather than nonspecific background staining. Isotype...structure, which means each IgM molecule has a binding capacity or valency of 10 antigens. An extra constant...

...mutated cancer genes are rare and most of the individual variants found in tumors are exceedingly rare...But, often these methods don’t agree. Confusing indeed! We've developed a new plasmid toolkit, the Broad...approach. Also, in principle, these experiments could find correlations between variants in a gene of poorly... next-generation of cancer patients. We hope you find these new cancer variant plasmid collections useful...

...(i.e. Csy4 cleavage sequence, tRNAs, multiple individual promoters).  spCas9 and its gRNAs are also larger...suitable substrate for Cpf1. This means Cpf1 can bind and cleave the lentiviral RNA, preventing packaging... with Cpf1 and GFP.  Of these neurons, ~15% had indels at all 3 targeted loci.  See the chart below for...Addgene.org Browse Our CRISPR Multiplexing Resources Find CRISPR Plasmids for Your Research Catch up on Your...

...creates more breakthrough and innovation. With any kind of open platform, there is the potential for increased...general auditor. I oversee a department of 20 individuals that help the plan manage risk. My job and my...LLP, auditing insurance, finance and high-tech industries. He has earned both an MBA in finance and a B.S...the other members of Addgene's Board of Directors Find careers at Addgene ...

...post is designed with beginners in mind, there’s some great reminders for Twitter pros as well, like tip...tips on using Twitter as a scientist. You can also find Dr. Page and Hello PhD on Twitter: @ThePurplePage...capture your interest during that week.” You can also find Dr. Knoepfler on Twitter: @pknoepfle 3. "Social ...

...replication, repairing mismatched basepairs or small indels that occur during DNA synthesis, and protomoting...processes (for example Dam and Dcm methylases) are independent from the restriction modification systems, yet...non-mutated and methylated template is destroyed leaving behind a pool of mutated plasmids which can later be verified...that is unmethylated at those sites. Please keep in mind that dam-/dcm- strains may have an increased rate...

...manuscript to make resources easier to identify, find back, and share. Depending on the type of manuscript...including F1000Research, also ask for the raw data behind the figures and results. This is a relatively new...researchers to access the data and make up their own minds on whether the data analysis has been of good quality...accepted for publication and is, in most cases, blind (meaning you don’t know who the reviewers are). ...

...manuscripts shared by the authors on a public server independent from peer-reviewed publishing in a journal.  ...is usually a confidential process among a few individuals.  Early-career researchers especially enjoy the...an updated preprint, further increasing their findability. Now, go out there and finish that experiment...10.1371/journal.pbio.3000151 Sever R, Roeder T, Hindle S, Sussman L, Black K-J, Argentine J, Manos W, ...

... Five: Share your results.  Does this process remind you of anything? Perhaps the Scientific Method?...expected to write up a report to communicate your findings to your colleagues. So perhaps a better definition...time in a lab learning techniques and taking on independent projects, you will inevitably develop problem...improve. The next time you face a problem, try to be mindful of how you work through it. Ask yourself if you...

...numerical designations, separated by a slash, that indicate the ITR and serotype, respectively. For example...polarities are rescued from the vector plasmid and individually packaged into the AAV capsids. As a genome conversion...scAAV) vectors promote efficient transduction independently of DNA synthesis." Gene therapy 8.16 (2001)....Elements Resources on Addgene.org Read Our AAV Guide Find AAV Vectors Browse All Viral Vector Resources ...

...you have to know what resources exist and where to find them! Keeping good records and an organized lab ... are typically more expensive, so you’ll have to find a balance between time savings and budget savings...factors to help you choose the right cloning method Find blog posts about scientific sharing here Download... out our special collection pages to more easily find plasmids Search for a plasmid in Addgene’s repository...

..., the lack of caveolin 1 in breast cancer also induces changes of stromal cells in the microenvironment...transforming growth factor-β signaling, possibly inducing the secretion of growth promoting proteins, such...molecules that can be transferred to other cells, inducing epigenetic changes in targeted cells. In this ...our cancer research plasmids and resources page  Find plasmids for your research Learn about Addgene's...

...some of its dsRNA intermediates into a healthy individual to provoke an immune response and then, hopefully...degrade mRNA transcripts. RNAi activates the RNA-induced silencing complexes (RISCs), which detect dsRNA...dysfunction, it is unclear what the precise mechanism behind the production of these dsRNAs is. A major source...infection. Similarly, if endogenously synthesized dsRNA finds its way outside of the mitochondria, the same inflammatory...

...neurons are likely the first thing that comes to mind. However, ion-based communication is not just for...reporting and genetically encoded fluorescent voltage indicators (GEVI) are ideal tools to track bioelectric states...channelrhodopsin used as a sensitive fluorescent voltage indicator with near-complete desensitization in continuous...BLINK2 potassium channel - An optimized blue-light induced synthetic potassium channel. Upon activation, it...

... on the Addgene Blog Find tips for online social networking for scientists Find tools for using social...profile is absolutely necessary. If people can’t find you on LinkedIn, you will lose opportunities and...for politics, family updates, or click bait of any kind. For more on inappropriate content see this article...

...Addgene.org Find protocols for plasmid cloning Read our molecular biology reference Find how-to videos..., Gibson Assembly, Golden Gate etc.), and try to find the simplest way to do it (i.e. avoid assembling...cloning strategy: Restriction enzyme digestion Be mindful of the restriction enzymes you chose. Make sure...