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  1. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...inactivation, protein degradation, and/or the use of chelating agents that can be introduced in the purification...time. Tris buffer controls the pH, while the EDTA chelates any divalent cations like Mg2+, preventing the...

  2. Fostering communication in remote collaborations

    Type
    Blog Post
    ...person, and helps improve your interpersonal relationships with people you’re collaborating with remotely...external collaborations Browse our science career related blog posts ...

  5. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...use of viruses is also important for social and regulatory reasons. 1.) The transposon consists of a mirrored...your gene-of-interest, either constitutively or regulated by doxycycline. The Sleeping Beauty transposase...

  6. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...backbones) are frequently used in molecular biology to isolate, multiply, or express the insert they carry in ... system is great for overexpressing YGOI in an isolated, controlled environment. In most cases bacterial...

  7. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...viral vector repository with a nice cup of hot chocolate is absolutely delightful. Grab a hot beverage ...forget to use the link below to keep up with the latest in Addgene's viral vector services and resources...

  9. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...genomes can be transcribed into mRNA, directly translated into proteins, or, in the case of retroviruses...which is then transcribed into mRNA before being translated into proteins. RNA viruses replicate their genomes...

  10. Summer SciComm: Science Storytelling

    Type
    Blog Post
    ...even by introducing a person that the audience can relate to. It doesn’t need to be anything fancy; it just... anthropomorphized animals, or even seemingly unrelated stories that they then tuck the science into. ...

  14. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ... projects studying developmentally programmed regulation of DNA replication and gene transcription during... useful and productive, forming a different constellation of interests and skills than what fits for applied...

  16. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...companies.  The majority of these meetings acted as a platform for significant company advancement. Be relentless... you’re able to answer every possible question related to it. First impressions are everything and you...

  17. Pushing the Limits of DNA Assembly

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    Blog Post
    ...DNA – perfect for transforma­­­tion or as a PCR template. Some of the applications of the NEBuilder HiFi...plasmids can be directly transformed into E.coli for plating, screening and generation of sgRNA. Generation ...

  19. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...the same myths and misconceptions fifteen years later. What does that look like?  One of my more recent...designs could work to slow the spread of illness. I later helped run workshops where I showed people how they...

  20. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...Sacks "Neurologist Oliver Sacks examines the relationship between the human brain and music: how we perceive...roles microbes play in our ecosystem, examining relationships between microbes and insects, mammals, and plants...
Showing: 721 - 740 of 968 results