We narrowed to 974 results for: LAT

1. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

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   Blog Post

   ... left four years later with a master’s. At the time my degree felt like a consolation prize, but in time...

2. Viral Vectors 101: Parts of the AAV Packaging Plasmid

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   ...proteins are also involved in the transcriptional regulation of the AAV p40 promoter which drives Cap expression...promoter. Alternative splicing and differing translational start sites generate the three VP proteins. ...

3. What's the Best Way to Elute and Store Your Plasmid DNA?

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   ...inactivation, protein degradation, and/or the use of chelating agents that can be introduced in the purification...time. Tris buffer controls the pH, while the EDTA chelates any divalent cations like Mg2+, preventing the...

4. Fostering communication in remote collaborations

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   ...person, and helps improve your interpersonal relationships with people you’re collaborating with remotely...external collaborations Browse our science career related blog posts ...

5. Bringing Sustainable Practices to the Lab: Recycling

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   ...researchers represent a minority of the world’s population, we account for an estimated 2% of global plastic...it has to be packaging (no coated tissue culture plates, for example). Check the link for more details ...

6. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

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   Blog Post

   ... to discover your insights on a topic. Beyond collating other’s data, you can even include your own data...patients was published, Zika researcher Christa Osuna collated Zika case studies, making a meta analysis available...

7. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

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   ... part of the job, but Caitlin doesn’t work in isolation. “A lot of what we do is work with students who...theses, and occasionally posting something science-related on Facebook. “I think this helped me say with confidence...

8. Sleeping Beauty Awakens for Genome Engineering

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   ...use of viruses is also important for social and regulatory reasons. 1.) The transposon consists of a mirrored...your gene-of-interest, either constitutively or regulated by doxycycline. The Sleeping Beauty transposase...

9. Choosing Your Perfect Empty Backbone

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   Blog Post

   ...backbones) are frequently used in molecular biology to isolate, multiply, or express the insert they carry in ... system is great for overexpressing YGOI in an isolated, controlled environment. In most cases bacterial...

10. New Viral Vectors - Winter 2025

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    Blog Post

    ...viral vector repository with a nice cup of hot chocolate is absolutely delightful. Grab a hot beverage ...forget to use the link below to keep up with the latest in Addgene's viral vector services and resources...

11. Viral Vectors 101: What is a Virus (and a Viral Vector)?

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    ...genomes can be transcribed into mRNA, directly translated into proteins, or, in the case of retroviruses...which is then transcribed into mRNA before being translated into proteins. RNA viruses replicate their genomes...

12. Science Careers: Unruly Interests Feed Many Paths

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    Blog Post

    ... projects studying developmentally programmed regulation of DNA replication and gene transcription during... useful and productive, forming a different constellation of interests and skills than what fits for applied...

13. What Do I Do Now? Academic v. Non-Academic Career Decisions

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    ...scheduling Diverse opportunities to do science related jobs that require no bench work Better resources... important first step. Developing mentoring relationships, especially with peer mentors, to discuss your...

14. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

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    Blog Post

    ... focus of the NABOR platform will be neuroscience reagents and data, the platform can be expanded for ...

15. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

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    Blog Post

    ...single mRNA translation in U2OS cells. HA is small, only 9 amino acids, thus it is translated quickly by...

16. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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    ...sorbitol is already a common excipient in AAV formulation buffers, this sugar may be a more attractive ...Charlotte A., Michael Hollinshead, and Amin Hajitou. "Clathrin-mediated endocytosis and subsequent endo-lysosomal...

17. Summer SciComm: Science Storytelling

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    ...even by introducing a person that the audience can relate to. It doesn’t need to be anything fancy; it just... anthropomorphized animals, or even seemingly unrelated stories that they then tuck the science into. ...

18. Donations from Addgene to Yield Answers for Rare Disease Researchers

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    Blog Post

    ...diseases, offering them the chance to win some of the latest tools and technologies in life sciences research...affected by AHC suffer from recurrent attacks of unilateral paralysis (hemiplegia) that are often painful...

19. Don’t Wait To Launch Your Health Science Startup

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    Blog Post

    ...companies.  The majority of these meetings acted as a platform for significant company advancement. Be relentless... you’re able to answer every possible question related to it. First impressions are everything and you...

20. Pushing the Limits of DNA Assembly

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    Blog Post

    ...DNA – perfect for transforma­­­tion or as a PCR template. Some of the applications of the NEBuilder HiFi...plasmids can be directly transformed into E.coli for plating, screening and generation of sgRNA. Generation ...