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We narrowed to 1,020 results for: NTS;

Showing: 721 - 740 of 1020 results

  1. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...resources continuously raising money from donations or grants to fulfill their mission. Addgene is fortunate ...-wide access to curated, high-quality data and reagents Addgene makes it easy for scientists to deposit...Twitter, Facebook, LinkedIn, email, or even the comments section of this post. We feel GREAT when you send...

  2. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...up the brightness in your fluorescent imaging experiments. Fluorescent protein fusions One of the easiest...Tanenbaum et al. performed a variety of different experiments in their paper, and so can you! In almost any...you to determine SunTag’s suitability in your experiments on a protein-by-protein basis. However, since...

  3. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...studies rely on delivering reversible or varied amounts of stimulation to receptors. Optogenetics offers...deschloroclozapine (DCZ) has been developed to meet these requirements. TL;DR If precise spatiotemporal control of...brainDissecting circuits Studying GPCR pathways   Acknowledgements We thank the Bryan Roth lab for feedback on...

  4. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...of your sample over time and determine which experiments you can effectively use the DNA. pH and DNases...protein degradation, and/or the use of chelating agents that can be introduced in the purification protocol...without having substantial degradation and which experiments you can use your plasmid.  DNA elution choices...

  5. Hot Plasmids - October 2022

    Type
    Blog Post
    ... use of HcKCR1 as an optogenetic silencer in experiments with mouse cortical neurons, far out-performing...two-photon excitation around 1,040 nm, has larger photocurrents, and is more selective for K+ than HcKCR2, making...long-sought tool for researchers to control K+ gradients and can be used as a potent optogenetic silencer...

  6. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ...biological sciences and constitute foundational elements of biomedical research. Traditional restriction...purification, and, most importantly, excessive time requirements. FastCloning is a simpler yet reliable cloning...directly into chemically competent E. coli and transformants selected with the appropriate antibiotic. Although...

  7. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ... variety in their job. Good relationships with clients and colleagues Sometimes this means you have to... in mind at all times when making decisions, assignments and polices. Sometimes the boss has to be the...knowledge-intensive workers”. I have added a few comments of my own to this list. Need feedback on their...

  8. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...to reuse plasticware or think about conserving reagents and buffers.  However, I had very environmentally... using.  I started to do little things to save reagents and money.  This was the start of my interest ...ethidium bromide, less stop buffer and miniscule amounts of plasmid sample.  How satisfying?  With practice...

  9. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...hooked up to invasive, cumbersome systems during experiments – neither ideal for the intrepid researcher. ...control – this is important later! Next, additional components are added - the ‘bells and whistles’ – including...on the other hand, was the wild west for fiber implants. Due to the soft nature of the intestines and ...

  10. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    ...RecA and RecBCD in bacteria. When two repeating elements love each other very much undergo recombination...sites, and other plasmids that contain repeating elements. Knowing how to amplify recombination-prone plasmids...save you the headache of troubleshooting failed experiments. Pick the right strain While common E. coli strains...

  11. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    ...reverse, making it unsuitable for some types of experiments. (To get more information on AraC and other promoter... aureochrome1 (VfAu1), led to functional BLADE variants, too. The team tested different linkers between... can do all of these more spatially confined experiments as well as those requiring reversibility that...

  13. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ...find details they need for planning their next experiments using a specific AAV tool. They can search the...tools. This word cloud (using wordart.com) represents contributed data on brain injection site from... a guide for designing your future injection experiments. Have your data or lab notebook handy?  Contact...

  14. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ... Getting a lot of empty vector contaminants in your cloning experiments and tired of having to PCR many...the coming years as we stay on top of the many developments in science and the plasmids, technologies, and...

  15. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...of their luciferase assay, they concluded that components in the buffer used for protein purification must... expression would require multiple transduction events. For these reasons, iTOP is best suited to binary...small molecule NSDB and Na+ hypertonicity are key elements of iTOP. iTOP can be used to transduce Cas9 and...

  16. Hot Plasmids - January 2023

    Type
    Blog Post
    ...candidate transcript targets analyzed. CRISPR-Csm presents an attractive alternative for nuclear-RNA targeting...hfYFP structure, the group rationally designed GFP variants with similar capabilities. If you’re looking for... temperatures, and in the presence of oxidizing agents. Adapted from Fig. 2 Campbell et al, Nature Methods...

  18. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    ...high-security facility needed to perform the experiments to demonstrate that this approach was useful ...diverse. Some of them are undergraduates or PhD students who are just starting their journey into science...stops these one-time favors from becoming starting points for long-lasting partnerships Join us! We are a...

  19. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...cells (as demonstrated in different cellular environments such as retina or glioblastoma). They also found...classical purification protocol involving iodixanol gradients. The current purification protocols for isolating...decipher the mechanistic aspects behind these improvements. What have exosome AAVs achieved so far? Exo-AAVs...
Showing: 721 - 740 of 1020 results