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We narrowed to 1,010 results for: PLE

Showing: 721 - 740 of 1010 results

  1. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...produced? Purifying exo-AAVs is actually a bit simpler than the classical purification protocol involving...have tried to modify it to improve yields. For example, transfecting the producer cell line with an expression...were always normalized to their genomic titers). Examples include the use of exo-AAV1 for transducing sensory...

  2. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ...techniques are continually being refined; it is completely plausible that scientists will eventually have...many things we can navel-gaze over from the last couple of weeks, including whether or not scientists can...history of medical research, there have always been people who unethically experiment on humans, and they ...

  5. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...about FPs than is currently represented at fpvis (complete spectral information, accession numbers, sequences...the goal of encouraging user contributions (using simple web forms) while also maintaining a relatively ...in the early stages of development, and there is plenty of room for improvement and elaboration.  Current...

  6. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...variants. Image adapted from Aggarwal et al…, Supplementary Figure 3.   Figure 3: pH titration...variants. Image adapted from Aggarwal et al…, Supplementary Figure 2.   Improved trafficking and localization... Multiple Authors...

  7. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ... most familiar bioluminescent organism for most people is the firefly (Photinus pyralis) and perhaps not...luciferase activity as a measure of signaling activity. Multiple response elements are cloned into a plasmid upstream... will result in luciferase expression and more complex cell treatments can be designed to further explore...

  8. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...For safety and production reasons, multiple plasmids with many components are used to package lab grown...with each other to allow DNA synthesis of the complementary strand to yield dsDNA. Unlike LTRs, ITRs are...): Cold Spring Harbor Laboratory Press; 1997. Principles of Retroviral Vector Design. Available from: ...

  9. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...researchers new to GCE aren’t able to successfully complete their experiments and many others are dubious ...universal techniques that researchers can easily implement in their own labs. They provide resources, knowledge...bulletin board on their website specifically so people can talk about GCE. In fact, their first protocol...

  10. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...cool because they can transmit information, for example, from one of the 180 regions to another or from...even further defined population of neurons. For example, say I wanted to access all the neurons that signaled... Multiple Authors...

  11. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...shRNAs are short RNA sequences which target complementary mRNA sequence for degradation. Viral introduction...targeting an exon in your gene of interest, for example. Some customization is required, but many viral...them an ideal delivery system for human patients. Multiple viral-based gene therapies have already been approved...

  12. How Addgene Creates Content

    Type
    Blog Post
    ... look at how we create this content, using the example of our Antibody Guide, which was published alongside...can. We even have a Slack channel just for asking people if they can write or review things and another ...require updating or retiring. CRISPR/Cas9, for example, has gone through rapid change in the past few ...

  13. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...specific region of your protein of interest. For example, if you are studying a protein that may bind a ...recognize the protein even though it is present in your sample. Additionally, there is a lower likelihood that...higher than that of polyclonal antibodies. For example, it takes longer to develop a hybridoma than it...

  14. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    ...of both AsLOV2 and the nanobody. As a proof-of-principle the Toettcher Lab monitored how AsLOV2 insertion...manipulation of the signaling pathway.  As a proof-of-principle, the team created an OptoNB to control the Ras...levers to pull to control signaling pathways. For example, if the OptoNB could bind and block a receptor ...

  15. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ... enzymes, some of which are also functional in multiple species (read Arbor Biotechnologies' press release...nt direct repeat) mediating potent knockdown. Multiplexing from the CRISPR array yielded >90% knockdown... necessary. Konermann et al. compared CasRx to multiple RNA regulating methods: small hairpin RNA interference...

  16. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...agriculture, are now synonymous with GM to the point that people cannot imagine any use for GM technology other ... is not food, not medicine, and not mandatory – people can decide for themselves if they want this beautiful...developed a genetic tool for plant engineering, please share it with Addgene to both build the plant synthetic...

  17. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ... the LoxP sites, which is more difficult than a simple knock out. Looking for some permanent knock out...transcripts. When transiently introduced, they can deplete endogenous protein levels for 1-3 days. There’... tunable control These systems can be easily implemented for exogenously introduced proteins, as many ...

  18. What's New in CRISPR - November 2021

    Type
    Blog Post
    ... further demonstrated that PRIME-del can be “multiplexed” using pooled paired pegRNAs.  Read the paper...Xue’s lab also uses paired pegRNAs targeting complementary DNA strands to program large genomic deletions... without small insertions. But, instead, PEDAR couples this deletion process to a prime editing enzyme...

  19. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...ever like to write about a recent plasmid deposit please sign up here.        AAV delivery of split base... Type IIS restriction sites to easily assemble multiple DNA fragments. This system has been used to construct...controlled by Cre recombination. Each cell can have multiple copies of the transgene that recombine independently...

  20. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ... practice to compare degradation rates between samples treated with or without an autophagy inhibitor....accumulates in the vacuole. See Figure 2 for an example of what fluorescence looks like for cytoplasmic...Fluorescent Protein Oligomerization Seeing Red: Simple GFP Photoconversion Light Sheet Fluorescence Microscopy...
Showing: 721 - 740 of 1010 results