We narrowed to 1,006 results for: RING;

...the body. Because calcium levels change rapidly during neuronal activity, scientists can use GECIs to ...shows: Higher ΔF/F0 (the measure of signal change during calcium events), Greater brightness, Less signal...

...are some good practice tips on adjusting voltage: During data acquisition, make sure that the signal for...upregulation or downregulation are easier to identify by comparing peak positions to the base expression. When compared...

...shock-inducible Cre and Cas9 for easy genome engineering in species like C. elegans and Drosophila. Light...  Mary Gearing...

... called the Resource Identification Initiative. During this project, authors are asked to add an identifier...guidelines for the reporting of laboratory animals during in vivo research and the CARE guidelines for case...

...however, they tend to be unstable and may be lost during budding. Yeast Centromere plasmids (YCp): These...with yeast, due to high rates of spontaneously occurring resistant mutants and the insensitivity of yeast...

...informing potential for success in a patient-mutation bearing knock-in mouse model for CTD. Congratulations the...questions,” Hébert says. In the spirit of plasmid sharing and open science, Hébert plans on building upon...

...techniques. Gel filtration and SDS-PAGE under non-denaturing conditions separate proteins based on size. Whether... and the concentration of the protein solution. During the experiment, the solution is diluted, complicating...

...similar to a Fab fragment but created through engineering instead of cleavage. They are comprised of the...stable up to 80°C and can refold correctly after denaturing.  Designed ankyrin repeat proteins, or DARPins...

...purification preserves RNA structure and naturally occurring complexes present in the cell, it can also introduce...events that occur on a short timescale, such as during drug action or cell signaling. Limitations of RaPID...

...as cereblon (CRBN) or von Hippel-Lindau (VHL) to bring the endogenous degradation machinery into close ...lentiviral plasmids can serve as a rapid means of comparing functionality of N- and C-terminal fusions and...

...you on LinkedIn, you will lose opportunities and hiring managers will think it is odd. For those planning...any of these people, including and especially the hiring manager. This is considered intrusive and it’s ...

...rather blunt instrument for controlling CRISPR. To bring greater control to Acr activity as a biological ...benthamiana or Arabidopsis thaliana (Xing et al., 2016)). During Agrobacterium-mediated DNA transfer, each GOI is...

... a musician, then metastasize is touring. But you know what a touring musician needs? A backstage team...

...spectral properties of the fluorophores you are considering to make sure that they’ll be compatible with ...target. I love FPbase’s Spectra Viewer tool for comparing different fluorophores. We also touched on fluorophores...

... could present something coherent or to quickly string together all the words that I could remember related...conferences, or even just provide quick updates during a company meeting increases visibility of your ...

...influence behavior. A conference and a breakthrough! During my graduate studies I attended a scientific symposium...inspiration to resolve the technical hurtle of monitoring protein turnover of specific proteins in situ...

...them for free. Plasmids ship out 2-3 days after ordering, making it a toss-up as to whether it’s faster...cloning method Find blog posts about scientific sharing here Download the Plasmids 101 eBook Resources...

...enough that there is virtually no chance of it occurring randomly. Therefore, inserting loxP sites at deliberate...are active only in certain cell or tissue types, during certain stages of development, or by making the...

...resulted altogether in 40 new ABE8 variants. When comparing on-target DNA editing efficiency in a human cell...and ABE7.10. They quantified genomic editing by measuring the reduction in protein expression of these six...

... of Research (FoR) symposia held across the USA during this time have revealed that young scientists are...Biochemistry and Molecular Biology/ FoR Hack Day during which several projects were initiated. Recent developments...