We narrowed to 1,045 results for: STI;

... instead that it is noninvasive and has longer-lasting effects. Building your chemogenetic system To set...they retain their intracellular interactions and still set off the same pathways, giving rise to the same... create ultrapotent PSEMS (uPSEMs) that have drastically increased potency. Download our chemogenetics...

...highlighted new tools but also included time for questions and a group discussion on the best strategies ... the Arizona State University (ASU) Biodesign institute developed a 3’UTR database specifically for C.... to activation sequences upstream of genes to stimulate expression (Wang et al., 2016). In 2018, the lab...

...include Swiss Webster and Harlan (Hsd) National Institutes of Health (NIH) Swiss. Genetically engineered...injection of a targeting vector alone, some challenges still exist. Nucleases may cause off-target effects on...the transgene in specific cells or under certain stimuli. Cre recombinase catalyzes the recombination of...

...and 5 lentiviral Destination vectors. These vectors can induce combinatorial constitutive or inducible gene...produce their shRNA library to support functional investigations of the mammalian genome that can reveal how...

...of expertise, equipment, or excellence of the institution). Likewise, if you’re staying in the same lab... applying for smaller grants from within your institute or a private funding body. Success breeds success...proof-of-principal study were very helpful in assisting me with applications for much larger fellowships...

... be used for the development of vaccines and diagnostics (Hsieh et al., 2020). As the virus spreads around...also deposited plasmids for producing COVID-19 diagnostic enzymes. Plasmids in this toolkit include ones...starting point for SARS-CoV-2 researchers to investigate how the virus interacts with human cells. Addgene...

...to keep track of growing collections of music. I still have a shelf full of CDs that have been gathering...Therefore, researchers from over 100 scientific institutions have proposed a shift to recombinant DNA-based...enormous amount of wasted money and time, with an estimated $350M/year lost on low quality antibodies in the...

...You’ve done great research and made interesting discoveries. You’ve analyzed the data and generated beautiful...should be easily navigable and efficient; your questions, data, and conclusions should be clearly identified...spacing between them so that they are easily distinguished. The UCLA Library offers some Powerpoint based...

...paternal grandmothers who experienced famine while still in their mother's womb passed on a trait to their...grandchildren; in this study,epigenetic changes were statistically significantly associated with reduced life-span...hybridization, methylation-sensitive restriction enzyme digestion, DNA adenine methyltransferase identification,...

...large heavy chains and two small light chains that stick out to form the “arms” holding the highly variable...the mRNA molecule, forming a ribosome complex consisting of the translated protein (sybody), ribosome,...constructed, they are used to produce a phage library consisting of phages that display the candidate sybodies...

...you’re currently using mVenus or mCitrine, or if existing yellow fluorescent proteins just haven’t met your.... The team used these libraries to analyze pre-existing heterogeneity of hematopoietic stem cells and ... S., & Rodriguez-Fraticelli, A. E. (2025). Pre-existing stem cell heterogeneity dictates clonal responses...

...AAV and lentivirus have low immunogenicity, AAV still beats lentivirus. In mice, AAV is almost entirely...high degree. This is particularly helpful when investigating tissue-specific diseases, as you can target ...doi.org/10.1038/s41596-022-00700-y Hanlon, K. S., Kleinstiver, B. P., Garcia, S. P., Zaborowski, M. P., Volak...

...sensing component, which separates them into two distinct categories detailed below. Voltage Sensitive Domain...fluorescence all have room for improvement. These sensors still have many useful applications, though, and if you...this two-part fluorescent system, and rhodopsin still functioning as the voltage sensor. FP-rhodopsin ...

...One of the most powerful strategies to investigate a gene's function is to inactivate, or "knockout",...(Figure 4). When recombination occurs, the gene still functions normally because one of its exons has ...are located in intronic regions, this gene will still be expressed. You'd first screen for this specific...

... Khan Khilji, a PhD student at the Max Planck Institute of Colloids and Interfaces. Here are some tips...wiped with 70% ethanol immediately. Finally, avoid sticking pipettes into solution bottles directly, rather...soon be joining her PhD lab at the Max Planck Institute of Colloids and Interfaces. Sana is particularly...

...a minute, when it is only warm but not hot- and still liquid- it is rapidly poured onto the middle of ...individual cell clones with different expression characteristics from a population of transfected clones. The...two 48-well plates of clones) which enables investigators to identify specific clones with desirable properties...

... In that case, an RQ value of one (or, more realistically, between 0.80-1.20) would mean you have one ...each reaction, math happens (this time, Poisson statistics), and the readout gives an absolute value of ...hg0721s80 Colborn JM, Byrd BD, Koita OA, Krogstad DJ. Estimation of copy number using SYBR Green: confounding ...

...handy guide from OpenWetWare for a more extensive listing of common E. coli strains. Increase the volume ..., based on determining the cell density or by harvesting and miniprepping at various time points. Measuring...Bacterial Transformation Learn How to Perform a Diagnostic Restriction Digest Additional Resources Sigma-Aldrich...

...localization, dynamics, and behavior of a POI. As an investigator, you want a fusion tag to be inert, to not produce...has gone into making FPs monomeric, yet many investigators remain ignorant of FP dimerization. Equally ... Jennifer Lippincott-Schwartz at the National Institutes of Health and he is currently an Associate Professor...

...vector Promoter driving Cas9 expression can be constitutive or inducible. U6 promoter is typically used ...will want to invest some time in optimizing and testing the efficiency of CRISPR delivery. There are a ... at Addgene that have been published as CRISPR testing tools: Traffic Light Reporter System: Can evaluate...