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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,043 results for: STI;

Showing: 721 - 740 of 1043 results

  1. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...include Swiss Webster and Harlan (Hsd) National Institutes of Health (NIH) Swiss. Genetically engineered...injection of a targeting vector alone, some challenges still exist. Nucleases may cause off-target effects on...the transgene in specific cells or under certain stimuli. Cre recombinase catalyzes the recombination of...

  2. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    ...you can make big changes in your career path and still find comfort and happiness there. Read all of the...part of why they work at Addgene. I posed this question to all nine of my interviewees. Fabienne Furt,...people.” My absolute favorite response to this question came from Lucy Hammer, our Financial Operations...

  3. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ... instead that it is noninvasive and has longer-lasting effects. Building your chemogenetic system To set...they retain their intracellular interactions and still set off the same pathways, giving rise to the same... create ultrapotent PSEMS (uPSEMs) that have drastically increased potency. Download our chemogenetics...

  4. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ... be used for the development of vaccines and diagnostics (Hsieh et al., 2020). As the virus spreads around...also deposited plasmids for producing COVID-19 diagnostic enzymes. Plasmids in this toolkit include ones...starting point for SARS-CoV-2 researchers to investigate how the virus interacts with human cells. Addgene...

  6. CRISPR in the Clinic

    Type
    Blog Post
    ...Repair of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem ...adult stem cells isolated from two patients with cystic fibrosis (Schwank et al). They were able to demonstrate...

  7. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...option in early 2020 and eventually replace the existing version of PubMed.  Key features This new version... for getting a quick summary of papers before investing the time to read them. Organizations: Accounts...AI-backed literature search developed by the Allen Institute for Artificial Intelligence. Semantic Scholar ...

  8. 6 Tips for Grant Writing

    Type
    Blog Post
    ...of expertise, equipment, or excellence of the institution). Likewise, if you’re staying in the same lab... applying for smaller grants from within your institute or a private funding body. Success breeds success...proof-of-principal study were very helpful in assisting me with applications for much larger fellowships...

  9. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...paternal grandmothers who experienced famine while still in their mother's womb passed on a trait to their...grandchildren; in this study,epigenetic changes were statistically significantly associated with reduced life-span...hybridization, methylation-sensitive restriction enzyme digestion, DNA adenine methyltransferase identification,...

  10. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...a minute, when it is only warm but not hot- and still liquid- it is rapidly poured onto the middle of ...individual cell clones with different expression characteristics from a population of transfected clones. The...two 48-well plates of clones) which enables investigators to identify specific clones with desirable properties...

  11. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...One of the most powerful strategies to investigate a gene's function is to inactivate, or "knockout",...(Figure 4). When recombination occurs, the gene still functions normally because one of its exons has ...are located in intronic regions, this gene will still be expressed. You'd first screen for this specific...

  12. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ... Khan Khilji, a PhD student at the Max Planck Institute of Colloids and Interfaces. Here are some tips...wiped with 70% ethanol immediately. Finally, avoid sticking pipettes into solution bottles directly, rather...soon be joining her PhD lab at the Max Planck Institute of Colloids and Interfaces. Sana is particularly...

  13. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...AAV and lentivirus have low immunogenicity, AAV still beats lentivirus. In mice, AAV is almost entirely...high degree. This is particularly helpful when investigating tissue-specific diseases, as you can target ...doi.org/10.1038/s41596-022-00700-y Hanlon, K. S., Kleinstiver, B. P., Garcia, S. P., Zaborowski, M. P., Volak...

  14. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...large heavy chains and two small light chains that stick out to form the “arms” holding the highly variable...the mRNA molecule, forming a ribosome complex consisting of the translated protein (sybody), ribosome,...constructed, they are used to produce a phage library consisting of phages that display the candidate sybodies...

  15. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ... In that case, an RQ value of one (or, more realistically, between 0.80-1.20) would mean you have one ...each reaction, math happens (this time, Poisson statistics), and the readout gives an absolute value of ...hg0721s80 Colborn JM, Byrd BD, Koita OA, Krogstad DJ. Estimation of copy number using SYBR Green: confounding ...

  16. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...sensing component, which separates them into two distinct categories detailed below. Voltage Sensitive Domain...fluorescence all have room for improvement. These sensors still have many useful applications, though, and if you...this two-part fluorescent system, and rhodopsin still functioning as the voltage sensor. FP-rhodopsin ...

  17. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...handy guide from OpenWetWare for a more extensive listing of common E. coli strains. Increase the volume ..., based on determining the cell density or by harvesting and miniprepping at various time points. Measuring...Bacterial Transformation Learn How to Perform a Diagnostic Restriction Digest Additional Resources Sigma-Aldrich...

  19. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    ...vector Promoter driving Cas9 expression can be constitutive or inducible. U6 promoter is typically used ...will want to invest some time in optimizing and testing the efficiency of CRISPR delivery. There are a ... at Addgene that have been published as CRISPR testing tools: Traffic Light Reporter System: Can evaluate...

  20. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    ...Wyss Technology Development Fellow at the Wyss Institute and Harvard Medical School. Scientists making ...efforts to develop formal guidelines to answer this question. Until then, using multiple confinement strategies...effort to implement (depending on the species in question), why not use them whenever applicable?Against...
Showing: 721 - 740 of 1043 results