Targeted Mutagenesis with EvolvR
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Blog Post
...within a defined region of a genome.
Require stringent bacterial growth conditions. Expression of an ...
We narrowed to 888 results for: TRI
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Plasmids 101: Screens vs. Selections
TypeBlog Post...selection can take the form of varied temperature, nutrient availability, drugs, and much more. Workflow... -
Antibodies 101: Isotypes
TypeBlog Post...edition. New York: Garland Science; 2001. The distribution and functions of immunoglobulin isotypes. Available... -
A Quick Guide to a Career in Software Product Management
TypeBlog Post...This post was contributed by guest blogger, Rachel Rubinstein, a field-based software product manager... -
Viral Vectors 101: Biosensors
TypeBlog Post...with dozens of sensors developed for it. Calcium triggers muscle contraction, and calcium channels are activated... -
Viral Vectors 101: Types of viruses
TypeBlog Post...efficiency. However, gamma-retrovirus infection is restricted to dividing cells only, so is not suitable for... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog Post...about a hundred billion neurons and six hundred trillion connections (synapses.) In this post we’ll describe... -
From Lab Bench to Leadership: Transitioning into Management as a PhD
TypeBlog Post...witnessed several colleagues do this while changing industries and positions. Some people discover they truly... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog Post..., so it no longer overlaps with the control. In tricky experiments, these populations may overlap slightly... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog Post...structural protein of the retroviral particle containing Matrix, Capsid, and Nucleocapsid components. Pol ... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog Post...multifragment assembly methods are preferred. No such restriction applies to deletions; using REPLACR-mutagenesis... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog Post...epitope. Addgene: Do you have any additional tips, tricks, or words of advice for people who may want to ... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog Post...of their CRISPR work in rice plants. Yang was intrigued by the tRNA processing machinery present in almost... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post...was a little like tapping in the dark - a lot of trial and error.” The structural details revealed two ... -
Dos & Don'ts When Publishing a Scientific Manuscript
TypeBlog Post...This post was contributed by Maaike Pols, PhD, a Developmental Editor at F1000Research. With many changes... -
RaPID Detection of RNA-protein Interactions
TypeBlog Post... with viral RNAs. RNA viruses, like Zika, often trick host proteins into binding their untranslated regions... -
Visualizing Protein Turnover In Situ
TypeBlog Post...This post was contributed by guest blogger, Eugenia Rojas. A question worthy of a PhD: How do you visualize... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog Post...package for therapeutic delivery by AAV, which has strict capacity limitations. New efforts have recently... -
Antibodies 101: Polyclonal Antibodies
TypeBlog Post...antibodies in its bloodstream, the antibodies are retrieved first by drawing blood from the immunized animal... -
Viral Vectors 101: An Introduction to AAV
TypeBlog Post...relevant viruses for your system. This post was contributed by guest blogger Didem Goz Ayturk with edits ...