We narrowed to 888 results for: TRI

1. From Lab Bench to Leadership: Transitioning into Management as a PhD

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   Blog Post

   ...witnessed several colleagues do this while changing industries and positions. Some people discover they truly...

2. Flow Cytometry Readouts: Yes, No, and Everything in Between

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   Blog Post

   ..., so it no longer overlaps with the control. In tricky experiments, these populations may overlap slightly...

3. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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   Blog Post

   ...structural protein of the retroviral particle containing Matrix, Capsid, and Nucleocapsid components. Pol ...

4. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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   Blog Post

   ...not be accessible by wild-type Cas9, due to its stringent PAM specificity. This can be especially important...

5. Targeted Mutagenesis with EvolvR

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   Blog Post

   ...within a defined region of a genome. Require stringent bacterial growth conditions. Expression of an ...

6. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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   Blog Post

   ...activity. This property makes WYL proteins an intriguing counterpart to anti-CRISPR proteins that negatively...

7. A Quick Guide to a Career in Software Product Management

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   Blog Post

   ...This post was contributed by guest blogger, Rachel Rubinstein, a field-based software product manager...

8. Viral Vectors 101: Biosensors

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   Blog Post

   ...with dozens of sensors developed for it. Calcium triggers muscle contraction, and calcium channels are activated...

9. Viral Vectors 101: Types of viruses

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   Blog Post

   ...efficiency. However, gamma-retrovirus infection is restricted to dividing cells only, so is not suitable for...

10. Viral Vectors 101: The AAV Retrograde Serotype

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    Blog Post

    ...about a hundred billion neurons and six hundred trillion connections (synapses.)  In this post we’ll describe...

11. Plasmids 101: Screens vs. Selections

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    Blog Post

    ...selection can take the form of varied temperature, nutrient availability, drugs, and much more.   Workflow...

12. Antibodies 101: Isotypes

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    Blog Post

    ...edition. New York: Garland Science; 2001. The distribution and functions of immunoglobulin isotypes. Available...

13. Plasmids 101: Inducible Promoters

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    Blog Post

    ...translation. Many thanks to Nicole Zurcher who contributed to the writing of this article. Additional Resources...

14. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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    Blog Post

    ...multifragment assembly methods are preferred. No such restriction applies to deletions; using REPLACR-mutagenesis...

15. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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    Blog Post

    ...epitope. Addgene: Do you have any additional tips, tricks, or words of advice for people who may want to ...

16. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    Blog Post

    ...of their CRISPR work in rice plants. Yang was intrigued by the tRNA processing machinery present in almost...

17. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    Blog Post

    ...was a little like tapping in the dark - a lot of trial and error.” The structural details revealed two ...

18. Dos & Don'ts When Publishing a Scientific Manuscript

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    Blog Post

    ...This post was contributed by Maaike Pols, PhD, a Developmental Editor at F1000Research.  With many changes...

19. Antibodies 101: Conjugation

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    Blog Post

    ...mechanisms. Most take advantage of chemical groups intrinsically present on antibodies, such as the primary amine...

20. RaPID Detection of RNA-protein Interactions

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    Blog Post

    ... with viral RNAs. RNA viruses, like Zika, often trick host proteins into binding their untranslated regions...