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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 968 results for: abo

Showing: 721 - 740 of 968 results

  1. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ... extremely heterogeneous. There is consequently about a two-thirds chance of causing a frameshift mutation...favor accumulation of the latter products. As noted above, a single cycle of cleavage and accurate repair ...

  2. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...communities. The ARRIVE guidelines for the reporting of laboratory animals during in vivo research and the CARE ...writing! Let us know if you have any other questions about writing and publishing your manuscript, or add your...

  3. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...in modern cloning, and why should you learn more about it? Read on to find out how ccdB can make your cloning...of this system for enhancing cloning efficiency about 20 years ago and developed cloning vectors to harness...

  4. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...they get old, they lose competency). If all the above is correct, you should probably have a second and...blog posts Read blog posts on plasmid cloning Read about different plasmid elements Resources on Addgene.org...

  6. All in a Twist: dsRNA

    Type
    Blog Post
    ...power of dsRNA for a long time, even before we knew about all the diverse in vitro production mechanisms. ...repetitive sequence. All of the R-loops described above play distinct roles in promoting cellular processes...

  9. Plasmids 101: Cre-lox

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    Blog Post
    ...discussed below. How does it work? As alluded to above, the Cre recombinase catalyzes the site specific...Combining this with some of the loxP methods described above, a genetic modification can be restricted based ...

  10. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...blog Find our CRISPR blog posts Read blog posts about using Cre-lox Read our genome engineering blog posts... posts Resources on Addgene.org Find more about Cre-lox Read our Genome Engineering Guide Learn more...

  11. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...available systems. Producing lentivirus: it's all about the T's (transfection and titers) To produce lentivirus... optimize transfection conditions. As mentioned above, plasmid ratios can be adjusted to enhance titer...

  12. Rabies and Neuronal Tracing

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    Blog Post
    ...connected to the neck. Shortly after, we learned about organs and the jobs they perform. This became foundational...part of the answer to many biological questions about the brain. To address this, molecular tools to map...

  16. Hot Plasmids - October 2020

    Type
    Blog Post
    ... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's...fluorescent proteins coupled with Fibronectin intrabodies generated with mRNA display (FingRs), Xue Han...

  17. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ...Bondy-Denomy et al., 2018). For example, AcrIIA4 mentioned above indicates that it’s the fourth Acr protein discovered...Cas9 Variant for Your Next Experiment Read more about Optogenetic Control of CRISPR   Resources on Addgene.org...

  18. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...Destination vector (dropdown options depending on above selections) Module set (the set of components you...vectors) Target type (drop down options depending on above selections.) CRISPR enzyme (Cas9 [sp], Cas9 [other...

  19. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...experiments, therefore, you often must make a choice about what is your top priority: location or efficiency...These Cas proteins have everything we know and love about Cas9, but with broader PAM compatibility. This alternative...

  20. CRISPR in the Clinic

    Type
    Blog Post
    ...trials The CRISPR therapies don’t stop with the list above! There are many, many more in the earlier stages... and others we just don’t have the time to talk about. Other treatments currently in clinical trials include...
Showing: 721 - 740 of 968 results