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TypeBlog Post...human therapies and in vivo laboratory experiments ranging from transient gene expression in a particular ...
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Transferable Skills Guide: Public Speaking
TypeBlog Post...semi-international audience or not in your own native language. And, at least in the biomedical field, with very... -
Plasmids 101: The protein expression toolbox
TypeBlog Post... its analogs will induce these transcriptional changes, and expression is tunable by titration with different... -
Antibodies 101: Polyclonal Antibodies
TypeBlog Post...low abundance or whose conformational state may change. If your protein might become slightly denatured... -
Celebrating 15 Years of Scientific Sharing
TypeBlog Post...The Atlantic. We’ve been in documentaries in 3 languages (Japanese, German, and English) and even on 60... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog Post...promotes gender equality in careers through structural change in research organizations. “Fix the Knowledge” ... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog Post...shape of the response function or how the output changes as a function of input. Usually that function is... -
Plasmids 101: Cre-lox
TypeBlog Post...experimental constraints. This has been used for a wide range of purposes, including activating an oncogene only... -
Lentivirus ddPCR Titration
TypeProtocol...outlined in this protocol is based on an LV titer range of 1E+05–1E+09 TU/mL, where TU is transducing units... This protocol was modified from the publication Wang et al. (2018) . Before Starting Thaw the master ...directly in the center of the well and tilt to a 45° angle. Count to 20 while slowly and gently pipetting the... of the well and tilt the pipette tips at a 45° angle. Count to 20 while slowly and gently dispensing ...Table: Example dilutions and titrations Reference Wang Y, Bergelson S, Feschenko M. Determination of Lentiviral... -
Adenoviral Vector Production and Troubleshooting
TypeBlog Post...confluent and the media will turn yellow. Do NOT change the media (add 2-3 mL of fresh media once a week... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog Post...Answering the big science questions around climate change, biodiversity, or genomes requires lots of data... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog Post...Timing: High expression levels of the viral vector trangenes typically occur two weeks after injection. Therefore... -
How to Prepare for an Industry Interview
TypeBlog Post...experience less stressful. First, you can make arrangements with those you live with to have a quiet place... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog Post...application (different experiments/conditions can change binding ability – i.e., the antibody can no longer... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog Post...affected, leading to unpredicted gene expression changes (Sapozhnikov and Szyf 2021, Charlton et al. 2020... -
Plasmids 101: Modular Cloning
TypeBlog Post...As a plasmid repository, we are no strangers to cloning. If you’re reading this, then chances are you... -
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog Post...concentration of any experimental wells in that range of optical densities. If your standard curve does... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog Post...With molecularly defined antibodies that do not change over time, scientists will know exactly what antibody... -
Early Career Researcher Toolbox: Finding Relevant Papers
TypeBlog Post...research or larger journals that publish about a broad range of topics. Twitter paper bots: Twitter bots tweet... -
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog Post...AAV vectors are also capable of infecting a wide range of cell types including but not limited to muscle...