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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 956 results for: cha

Showing: 721 - 740 of 956 results

  2. Addgene: The First Twenty Years

    Type
    Blog Post
    ...MTA system. We also started our blog and YouTube channel in 2013, which continue to grow today. “I generally...Neuroscience 2023 conference. In 2020, the world changed in response to the COVID-19 pandemic, and Addgene...

  3. Human Germline Editing Using CRISPR

    Type
    Blog Post
    .... Created with BioRender.com. In later characterization studies, the edited embryos proceeded to the...heterozygous mutations, it will clearly be much more challenging for homozygous mutations. Moreover, introducing...

  4. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...fluorescent measurements from red, blue, and green channels overlaid on a bright-field image. Image from Ma...intrachromosomal loci, a major benchmark in characterizing the 4D nucleome. CRISPRainbow: Scaling up to...

  5. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ... which target and cleave RNA through the same mechanism as traditional spCas9. If you are looking to modify...it perfect for targeting mRNA and useful as a mechanism of transient knockdown of gene expression. This...

  6. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...recently identified Cas12f systems from uncultivated archaea are much smaller and would be ideal for such AAV...system (CasMINI) engineered from the uncultivated archaea Cas12f (Un1Cas12f1, or Cas14a1) system. The CasMINI...

  7. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...common method. These proteins bind the IgG heavy chain. Running the serum over a column of resin crosslinked...low abundance or whose conformational state may change. If your protein might become slightly denatured...

  8. RNA Extraction Without A Kit

    Type
    Blog Post
    ...something that broke down RNases before RNases had a chance to break down RNA upon cell lysis.  In the late...until homogenization and cell lysis. This can be challenging depending on your laboratory situation or tissue...

  10. Plasmids 101: Cre-lox

    Type
    Blog Post
    ..., and is long enough that there is virtually no chance of it occurring randomly. Therefore, inserting ...recombination event in a single system. The Saccharomyces cerevisiae FLP-FRT recombination system is another...

  11. Design Tips for Prime Editing

    Type
    Blog Post
    ...edited and the sequence of the edit, so its design changes for every new prime editing application. And optimizing... L., Schmidt, R., Solley, S. C., Gilbert, L. A., Chan, M. M., Bauer, D. E., Marson, A., Parsons, L. R....

  12. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...patient mutation or need to make a precise amino acid change, there is very little flexibility in the location...nucleotides are represented equally, that’s only a 1/16 chance of having a PAM at a given site. While it is possible...

  13. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...sequencing of neurons from each class to fully characterize the transcriptome. The first data release for...C.elegans neural network, and Sibernetic, a fluid mechanics simulator that can be used to simulate muscle ...

  14. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...derived from random crosses between mice of uncharacterized backgrounds to create a limited but genetically...the injection of a targeting vector alone, some challenges still exist. Nucleases may cause off-target effects...

  15. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...not integrate into the host’s genome. The main challenge with using AdV is that it triggers a strong immune...confluent and the media will turn yellow. Do NOT change the media (add 2-3 mL of fresh media once a week...

  17. Lab Automation at Addgene

    Type
    Blog Post
    ...spirit include: Start using electronic multichannel and single channel pipettes. These can speed up any project...

  19. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...broadly reactive secondary antibody to increase the chance of primary/secondary antibody binding and therefore..., P. A., Lewis, G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical Techniques ...

  20. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ... wells with a purified protein allows you to characterize properties of an antibody that recognizes that.... As mentioned above, it can also be used to characterize antibodies against the antigen bound to the ...
Showing: 721 - 740 of 956 results