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Find tips for working with RNA and simple protocols for extracting RNA without using a kit

Getting start with mouse models? Here, we break down how to cross mice to create double knockout lines and Cre-lox lines.

Rosella is a fluorescent biosensor that you can use to study autophagy.

What is a career coach and how can scientists find the right one for them?

There are a few different types of Fab fragments to consider when you need a smaller affinity reagent than antibodies. Learn about them here!

An updated look at how CRISPR is being used in therapeudic applications across a variety of diseases.

ELISA are used to quantitatively detect protein concentrations in experimental samples. Learn about different types of ELISA and when to choose each method.

If you're new to behavioral neuroscience, this article will walk you through how to standardize your experiments and chose viral vectors appropriate for your experiment.

Learn what FRET is and how you can use it in your experiments. Find additional FRET resources throughout the post.

Dora Farkas, career coach for scientists, discusses how to identify difficult supervisors and outlines 7 strategies to resolve conflicts.

Western blots have many technical elements. We cover each element, and the different options available to choose from, in this post.

This protocol describes the basic steps for fixing and staining cells in culture with a primary antibody against a target protein and a fluorescent secondary antibody.

Did you know CRISPR can be used to target non-coding RNAs? In this blog post, we explain the pros and cons of different approaches.

Find the basics about chemogenetics: what are chemogenetics, why use them, and how to build your chemogenetic system using receptors and ligands.

An in-depth look at fixing and permeabilizing samples in immunofluorescence experiments.

an overview of qPCR and ddPCR copy number variant assays.

Our new Instagram series, Meet an Addgenie, features stories form Addgenies about their career decisions, paths, and more.

A new open source tool for CRISPR experiments, CrispyCrunch, has been developed by the Chan Zuckerberg Initiative to design batch gRNAs, templates, and sequencing primers for CRISPR + HDR experiments.

Learn how a 3D protein model can help you visualize, learn, and teach others about protein structure and function.

Get your fly research started with this quick guide to working with drosophila.