We narrowed to 559 results for: Spr;

... observed light emission by fluorescence. The widespread use of FRET with fluorescent molecules, including...cascades, quantify mechanical tension (a molecular “spring”) (Meng et al., 2008), and monitor neuronal activity...

...For instance, through open plasmid sharing, the CRISPR community has created hundreds of useful variants...Antibodies: a Laboratory Manual, 2nd Edition. 2104. Cold Spring Harbor Laboratory Publications, New York. Helma...

... of the plasmid in the pool - ex. the gRNA in a CRISPR library. In general, our QC scientists work with...Brigham MD, Sanjana NE, Zhang F (2017) Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...

...intracerebroventricular) to minimize tissue damage; however, the spreading of vector to the area of interest may be very ...downregulation via RNAi or gene editing/deletion using CRISPR. In some cases, a decrease of the expression efficiency...

... TnpB, so hot right now By Alyssa Shepard While CRISPR has become a household name, the search for smaller...more in our recent blog post on TnpB). Similar to CRISPR-Cas, TnpB is directed to genomic targets using ...

... only the cultures with intact ITRs. I use a spreadsheet similar to this one to plan my AAV packaging ...packaging transfections Find tips for a first time CRISPR user Resources on Addgene.org Learn how Addgene...

...have fly collections for in vivo work, including CRISPR tools. The Vienna Drosophila Resource Center ...Addgene.org Find Drosophila Plamids Drosophila CRISPR Resources Deposit Your Drosophila Plasmids ...

...the community. I even spray painted a small erlenmeyer flask with gold glitter spray paint to make a trophy...

...expression of KRAB-dCas9-DHFR for CRISPRi. See Plasmid #167937 for CRISPRa Emma Rawlins 72835 pMK243 (Tet-OsTIR1...-inducible Cas9-2A-EGFP expression. Based on LentiCRISPR v2 . Adam Karpf 167935 pLenti-tetON-KRAB-dCas9...

... (2002) Arabidopsis : a laboratory manual. Cold Spring Harbor Laboratory Press. Available at: https://...Addgene blog Engineering the plant genome using CRISPR/Cas9 Using pSiM24 for simplifying plant genetic...