Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 968 results for: LAT;

Showing: 741 - 760 of 968 results

  1. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...is a challenge. To help keep you abreast of the latest fluorescent proteins, I maintain an interactive... is sufficient to use a spectrum for a closely related protein, if you know that your protein of interest...

  2. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...pub with us in the evening, wanting to know our latest results as soon as the gels ran.” Dr. Ellis tries...emphasis on genetic engineering, sequencing, and translation of results to companies.” Take home message Starting...

  3. Antibodies 101: Isotypes

    Type
    Blog Post
    ...region length. Now for some essential isotype nomenclature. Antibody heavy chain proteins as well as the...start by co-expressing IgM and IgD antibodies, but later in an immune response, the variable region of an...

  4. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...adding more terms.  Weighting We determined the relative importance of different bits of information for...shortcuts to specific catalog items as well as related items (for example, a kit that contains a plasmid...

  6. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...offered by the system that allows for specific manipulation of neurons. We have expanded our AAV collection...Chemogenetics offers noninvasive methods for manipulating cell activity, particularly in the brain, and...

  7. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...in the native virus. Since the WT sequence is relatively unstable, a more stable 130 bp version is used... D. V. (2023). AAV- based vector improvements unrelated to capsid protein modification. Front Med, 10,...

  9. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...continuously imaged cells for about 4 hours! The latest enhanced Nano-lanterns (eNL) described in Suzuki...signals of various Nano-lanterns, but it does not isolate each Nano-lantern, so Takai et al. used a linear...

  11. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...? Sometimes, the funders of your research may stipulate that your article must be published in an open...data behind the figures and results. This is a relatively new development, and it allows reviewers and ...

  12. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...antibodies to antibody fragments to tools completely unrelated to antibodies.  Why use an alternative affinity... These are antibody mimics and are not closely related to antibodies. Instead, they are derived from a...

  13. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...the requirements of the specific assay. For the latter three, secondary antibodies are indicated when ...absolute quantitative analysis of a sample. Instead, relative quantification can be done using either the direct...

  15. Click Biology: How to Click and Play to Explore Biology

    Type
    Blog Post
    ...homology-directed repair efficiency by linking the repair template VirD2 HUH-tag For creating DNA-protein conjugates... are many convenient tools out there, not just relating to proteins but also covalent reactions to DNA...

  16. Addgene: The First Twenty Years

    Type
    Blog Post
    ...the Neuroscience AntiBody Open Resource (NABOR) platform, supported by an NIH BRAIN Initiative. We also...shipped out our two millionth item, and had a cumulative 120,000 citations of individual items in our ...

  17. dTAG - You're it!

    Type
    Blog Post
    ...FKBP12F36V-tagged proteins using our lentiviral expression platform, to assess selective degradation of FKBP12F36V-BRD4...dTAG-47 Weintraub et al. YY1 Is a Structural Regulator of Enhancer-Promoter Loops. Cell. December 14 ...

  19. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...cloning, perform an exonuclease cleanup, transform, plate, and pick. In short, the Fragmid system takes the...Plasmid pRDB_053 (for transgenic flies; gypsy insulator sequences; white + selectable marker; attB site...

  20. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...the membrane for storage, but plan to reprobe it later, strip it thoroughly first. Then continue on with...always be added fresh to solutions. It is also volatile and toxic (and smells bad!), so be sure to use...
Showing: 741 - 760 of 968 results