We narrowed to 1,073 results for: TOR

...resources: Preserving scientific assets: We hold a repository of valuable research materials that might otherwise...continue to offer high-quality DNA sequencing, viral vector preparations, and antibody production that labs...continue.  Moving Forward Together Throughout history, the scientific community has demonstrated remarkable...

...the virus itself that kills you, but the cytokine storm it ultimately sets off.  The virus works its way...cells and turn the cell into a veritable virus factory.  Their findings were reported in the Journal of...and the target of neutralizing antibodies and inhibitors of attachment and fusion. Recommended Reading...

...two options: “Search Repository” and “Deposit Materials”. The “Search Repository” function is our same...section: Tools of the Day. Our plasmid and viral vector of the day have a fresh new look and a fresh new...

...'Regan Ordering jeans online from your favorite store is easy. Ordering genes on plasmids online is a ...nonprofit, Addgene does not own the material in our repository. We distribute it on behalf of depositing institutions...full of information about how to deposit plasmids, store your plasmids or viral items, technical support,...

...Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins by Cary Valley,...Academic Job Interview by Erik Snapp Addgene depositor and guest blogger Erik Snapp breaks down how to... blog posts Resources at Addgene Browse the repository Find educational resources at Addgene Search ...

..., even when all kinds of other factors, including journal impact factor, study topic, and the corresponding...preference to journal access granted via a public repository or authors’ own web sites. 9)      The year 2013...

...MCF10A cellular models. Ba/F3 and MCF10A are growth factor dependent cell lines and any mutations that that...advantages will be revealed in the absence of growth factor. The team assigned the mutations to functional ...significantly increased cell viability, and these “activators” may be the direct drivers of tumor formation...

..., and young Principal Investigators (PIs). We've interviewed Addgene depositors who are at various stages...different ways to succeed – as loner or gregarious;  dictator or laissez-faire; theory or practice; business-like...

... gene in a pLenti vector backbone. During those five weeks, I worked with my mentors, Katherine, Amrita...exchanged contact with Amy C., Addgene’s intern coordinator shortly after hearing her talk about Addgene’...

...used to target complementary DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA...array: The section of the bacterial chromosome that stores a genetic record of previously encountered foreign...itself, simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer ...

...original policy was straightforward, but cumbersome: depositors had to email us with permission to release their... are left in the hold status and reach out to depositors about them, but we all know how easy it is to...your work. Plus, it’s costly for us to process and store materials that aren’t distributed or for our teams...

...affiliated TniQ protein recruits transposition cofactors. The transposon proteins integrate a payload sequence... Figure 1: Mechanism of CAST systems. A Cas effector (Cascade in Type I systems; Cas12k in Type V-K...Programmable gene insertion in human cells with a laboratory-evolved CRISPR-associated transposase. Science...

...stuffing new depositor welcome kits, note taking for protocol video production, and keeping inventory of our... the people at Addgene have become my friends, mentors, and some of my favorite people to talk to. This...

...match sequence information contributed by the depositor, published backbone sequences, and any known sequences...analyze sequencing results for plasmids in the repository at a glance. Our Snapgene-powered plasmid maps...experiments like restriction digests. We’ll have a tutorial showing all of the features available on our Snapgene...

...neurons that trigger the CPG (central pattern generator) (Wyart et al., 2009). The CPG is a neural circuit... involved in locomotion that generates periodic motor commands for rhythmic movements like spontaneous...optogenetics blog posts Read blog posts about viral vectors Subscribe to the Addgene blog Resources on Addgene.org...

...biodistribution therefore introduce additional confounding factors when attempting to interpret signals from these...collapse E. coli re-express arg1 gas vesicles and restore their ultrasound signal. Using acoustic reporter...years to clinically treat enteric infection and inflammatory bowel conditions. In these experiments, arg1...

...department.” Caitlin does this by acting as a facilitator between her team, the sales department, and operations...business.” Caitlin’s advice for budding science communicators: “My recommendation would be to take any opportunity...education tools? Check out this Addgene podcast with educator and science rap mastermind Tom McFadden Get tips...

...For this series, I’ll interview three science communicators who work in the biotech, education, or nonprofit...job. “I am really more of a multimedia science communicator than a science writer these days. I do some ... to talk to other scientists when I am in new territory. Perhaps there are examples of science writers...

...cannot produce them. By using yeast integration vectors (see for example this yeast cloning kit) she intends...expression system such as the pET GFP LIC cloning vector and the pOKD plasmids. The recombinant Cry proteins... develop technologies to protect fresh water repositories in Mexico. At Addgene we're happy to be able...

...individuals through stock or investor pay outs. Addgene has no investors or stock. All of our proceeds...proceeds go into running the repository and serving scientists better. That includes keeping fees as low as ...