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We narrowed to 1,010 results for: APP

Showing: 741 - 760 of 1010 results
  1. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...workflow - analyzing the data - is often under-appreciated or over-looked.  Synthego has developed a new...analysis Once the analysis is complete, a new screen appears with a graphical representation of the results ...
  2. How to Write a Scientific Review Article

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    Blog Post
    ...papers and then feel ready to write. What actually happened was that each paper taught me a few things and...to read every single paper. I also learned to appreciate the discussion sections of the primary literature...
  3. Plasmids 101: Gateway Cloning

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    Blog Post
    ...sequences into multiple vector systems. With the appropriate entry and destination vectors, one can use Gateway...elements to express the gene of interest in the appropriate system Recombines with the entry clone to generate...
  4. Antibiotic Resistance: An Old Solution but a New Problem

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    Blog Post
    ...from random mutations in the genetic sequence that happen to be beneficial, and persist as a result. Why ...are susceptible to the drug, while the few that happen to possess resistance genes survive and multiply...
  5. CRISPR Activation: A Practical Guide

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    Blog Post
    ...suffer from an inability to easily deliver the appropriate isoform(s) of a given gene, as, many times, the...engineering parameters via a library-on-library approach. Nat Methods 12:823–826 . https://doi.org/10.1038...
  6. Neuronal labeling with Spaghetti Monster

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    Blog Post
    ...fluorescent proteins (FP) have also been important in mapping, monitoring, and manipulating neuronal circuits...combining advantages of FPs and epitope tags, smFPs appear to be very effective probes for finely visualising...
  7. Plasmids 101: Control Plasmids

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    Blog Post
    ...RNAi or CRISPR, non-targeting controls may be appropriate as they allow you to assess the specificity of...us now revisit our experiment. In Figure 1, it appeared as though the shRNA did not knock down expression...
  8. Plasmids 101: E. coli Strains for Protein Expression

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    Blog Post
    ...plasmid-- make sure to maintain this by growing on appropriate media. Note: Purifying your expression plasmid...to make sure your cells have first reached an appropriate density. Cells in the exponential growth phase...
  9. Using a Light Microscope Protocol

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    Protocol
    ...see your image through both sides. Once you are happy with the lighting, use the coarse focus knob to ...make minor adjustments to the focus. After you are happy with the positioning and focus of your image on ...microscope after use and store the microscope appropriately....
  10. Using a Light Microscope Protocol

    Type
    Protocol
    ...see your image through both sides. Once you are happy with the lighting, use the coarse focus knob to ...make minor adjustments to the focus. After you are happy with the positioning and focus of your image on ...microscope after use and store the microscope appropriately....
  11. Handling Plasmids from Addgene - Purifying Plasmid DNA

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    Protocol
    ...overnight culture of bacteria . Pro-Tip Refer to appropriate DNA prep protocol for volume of bacteria to grow...the plasmid DNA. Either spin to pellet the DNA or apply the solution to a column that will bind the now ...Aqueous DNA phase Middle phase - A white layer may appear, consisting of precipitated protein particles Bottom...
  12. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...overnight culture of bacteria . Pro-Tip Refer to appropriate DNA prep protocol for volume of bacteria to grow...the plasmid DNA. Either spin to pellet the DNA or apply the solution to a column that will bind the now ...Aqueous DNA phase Middle phase - A white layer may appear, consisting of precipitated protein particles Bottom...
  13. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...Escherichia coli as a Model Organism and Its Application in Biotechnology. In Escherichia coli - Recent...Physiology, Pathogenesis and Biotechnological Applications. InTech. https://doi.org/10.5772/67306 Koornneef...
  14. Plasmids 101: Expressing shRNAs for RNA Interference

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    Blog Post
    ...world, there is a myriad of tools this could be applied to. When it comes to altering gene expression, ...is an umbrella term for a number of different applications that involve different types of small RNA molecules...
  15. Transferable Skills Guide: Time Management

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    Blog Post
    ... cell splitting issue at all if I’d planned appropriately. Back in my undergrad days, I had general goals... do I write things down? It’s more viscerally appealing for me to physically cross things out (it just...
  16. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    Blog Post
    ...that expresses all desired variants of a protein. Applying a selective pressure winnows the pool down to ...technique described in Wrenbeck et al is a PCR-based approach for generating a customizable comprehensive mutagenesis...
  17. Fluorescent Proteins 101: When GFP lets you down

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    Blog Post
    ... proteins.Therefore, GFP is not suitable for applications that require anaerobic conditions. In experiments...protein module that can report on location. This approach enables the study of proteins in cells, but not...
Showing: 741 - 760 of 1010 results