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...adjust this setting as needed. To figure out the appropriate voltages to observe your cells on a flow plot...voltage describes an electric potential that can be applied to the photomultiplier inside the cytometer to ...

...discounts, collaborations, access to internal award applications, etc. If this is the institute that can help...budgets and invite you to leave a comment below Happy Sciencing, Damien   Damien Wilpitz is a laboratory...

...something odd in our uncut DNA control: a band or two appear on an agarose gel at notably higher molecular weights... only in the uncut DNA; the rest of the digest appears normal and produces the expected fragments for ...

...extensively characterized and validated, with the application data from IPI and other labs easily viewed in...2021). Directed Evolution: Methodologies and applications. Chemical Reviews, 121(20), 12384–12444. https...

...immunotherapeutic agents. Currently the FDA has 12 approved monoclonal antibodies targeting the PD-L1 pathway...Dendreon Corporation is currently the only FDA-approved DC therapy for prostate cancer and is aimed at...

...complete: Prepare 7 microcentrifuge tubes with the appropriate amount of media volume listed in the chart below...tube. Add 4 µL of the 25 ng/µL samples to the appropriate PCR tubes. Pipette back and forth 10 times to...the average infectious titer obtained from the appropriate dilutions to calculate the final infectious titer...

...with the newest CRISPR technologies and their applications can be exhausting. A quick search for "CRISPR...Laganière, J., Cheng, A. W., Hockemeyer, D., Gao, Q., Alagappan, R., Khurana, V., Golbe, L. I., Myers, R. H., ...

...within the entire construct. Is the primer an appropriate distance from the target? The primer should be... should I do? Check your trace file first; the apparent mismatch/mutation may be the result of a mis-called...

...in place. Turn the water bath on and set the appropriate temperature as described in your protocol. How...in step 3. You will also need to maintain the appropriate water level in the water bath so before use, ...

...traditional cloning, base-pairing in the short overlapping regions (usually 4 bp) does not provide enough...with the start codon or tag sequences (where appropriate). The primer length is dependent on the T4 Pol...

...fragment length or end compatibility, multiple overlapping DNA fragments can be joined in a single isothermal...part to be inserted is too long to include on overlapping PCR primers (>60 bp) but too short to make its...

...lentivirus can be used for a variety of downstream applications such as stable-cell line generation. Workflow...mL of DMEM Complete to a 15 mL conical and an appropriate amount of 25 mM chloroquine to the conical so...

.... Add 1.5 mL of DMEM complete containing the appropriate antibiotic. Incubate the cells for ~2 weeks. ...replace it with fresh DMEM complete containing the appropriate antibiotic. When the untransduced (negative control...

... P1000. The table shows the pipette with the appropriate dispense volume: This image shows the P2, P10...,000 µL Each pipette should be used with the appropriate tips, which are made in a number of sizes and...

...want a recipient plasmid to insert ratio of approximately 1:3. Since the number of base pairs for each...should also verify that you are plating on the appropriate antibiotic and try varying the amount of recipient...

... can be adapted for wet and semi-dry transfer approaches. Last Update: January 24, 2022 Workflow Timeline...remove supernatant. Resuspend cell pellet in appropriate volume of cold lysis buffer. Pro-Tips The volume...

...Water, Hyclone, SH30538.02 GeneAmp 10X PCR Buffer, Applied Biosystems, N8080129 ddPCR Supermix for Probes ... PCR tubes. Add 5 µL of dilutions 6–8 to the appropriate PCR tubes. Pipette back and forth 5 times. Lightly...

... not affect the quality of RNA or downstream applications. To improve yield of RNA, instead of incubating...

... Western blot and share tips for this tricky application.    You can check out all of our protocols ...

...found on our Protocols page and YouTube page.  Happy learning, and welcome to the lab bench, new scientists...