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Showing: 741 - 760 of 964 results

  1. Viral Production at Addgene

    Type
    Blog Post
    ...particles can be identified after negative staining and appear darker than full vector particles.     ...replaced with a new vial of early passage cells. Approximately two weeks post-thaw, cell culture supernatant...

  2. How to Be an Excellent Trainee

    Type
    Blog Post
    ...easiest to train individuals who are polite, appreciative, and clear about their needs. Remember, your...trainees one day when you are the senior personnel! Happy training! Resources Resources on the Addgene blog...

  3. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...bioinformatic analysis. At Addgene, we use a mix-and-match approach for sequencing methods and choose the best method...Nanopore sequencing technology, bioinformatics and applications. Nature Biotechnology, 39(11), 1348–1365. https...

  4. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...suffer from an inability to easily deliver the appropriate isoform(s) of a given gene, as, many times, the...engineering parameters via a library-on-library approach. Nat Methods 12:823–826 . https://doi.org/10.1038...

  5. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...RNAi or CRISPR, non-targeting controls may be appropriate as they allow you to assess the specificity of...us now revisit our experiment. In Figure 1, it appeared as though the shRNA did not knock down expression...

  6. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...plasmid-- make sure to maintain this by growing on appropriate media. Note: Purifying your expression plasmid...to make sure your cells have first reached an appropriate density. Cells in the exponential growth phase...

  7. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...add liquid LB to a tube or flask and add the appropriate antibiotic to the correct concentration ( see...Large plasmids usually have a low copy number (approximately one or two copies per cell) and they need to...to grow for longer periods of time (approximately 18-30 hr). On the other hand, smaller plasmids can be...

  8. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...protocols that you can use for a wide range of applications, with videos for select protocols in the right-hand... of DNA to a plasmid Gibson Assembly Combine overlapping DNA fragments in a single reaction Ligation Independent...preparation Antibodies Protocols for common antibody applications. Name Description Link to Video Transfection...

  9. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ... proteins.Therefore, GFP is not suitable for applications that require anaerobic conditions. In experiments...protein module that can report on location. This approach enables the study of proteins in cells, but not...

  10. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ... cell splitting issue at all if I’d planned appropriately. Back in my undergrad days, I had general goals... do I write things down? It’s more viscerally appealing for me to physically cross things out (it just...

  11. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...Escherichia coli as a Model Organism and Its Application in Biotechnology. In Escherichia coli - Recent...Physiology, Pathogenesis and Biotechnological Applications. InTech. https://doi.org/10.5772/67306 Koornneef...

  12. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...that expresses all desired variants of a protein. Applying a selective pressure winnows the pool down to ...technique described in Wrenbeck et al is a PCR-based approach for generating a customizable comprehensive mutagenesis...

  13. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...adjust this setting as needed. To figure out the appropriate voltages to observe your cells on a flow plot...voltage describes an electric potential that can be applied to the photomultiplier inside the cytometer to ...

  14. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...world, there is a myriad of tools this could be applied to. When it comes to altering gene expression, ...is an umbrella term for a number of different applications that involve different types of small RNA molecules...

  15. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...discounts, collaborations, access to internal award applications, etc. If this is the institute that can help...budgets and invite you to leave a comment below Happy Sciencing, Damien   Damien Wilpitz is a laboratory...

  16. Using a Light Microscope Protocol

    Type
    Protocol
    ...see your image through both sides. Once you are happy with the lighting, use the coarse focus knob to ...make minor adjustments to the focus. After you are happy with the positioning and focus of your image on ...microscope after use and store the microscope appropriately....

  17. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...something odd in our uncut DNA control: a band or two appear on an agarose gel at notably higher molecular weights... only in the uncut DNA; the rest of the digest appears normal and produces the expected fragments for ...

  18. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...overnight culture of bacteria . Pro-Tip Refer to appropriate DNA prep protocol for volume of bacteria to grow...the plasmid DNA. Either spin to pellet the DNA or apply the solution to a column that will bind the now ...Aqueous DNA phase Middle phase - A white layer may appear, consisting of precipitated protein particles Bottom...

  19. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...extensively characterized and validated, with the application data from IPI and other labs easily viewed in...2021). Directed Evolution: Methodologies and applications. Chemical Reviews, 121(20), 12384–12444. https...

  20. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...immunotherapeutic agents. Currently the FDA has 12 approved monoclonal antibodies targeting the PD-L1 pathway...Dendreon Corporation is currently the only FDA-approved DC therapy for prostate cancer and is aimed at...
Showing: 741 - 760 of 964 results