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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,019 results for: CAL;

Showing: 741 - 760 of 1019 results

  1. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...uses a “nicking” Cas9, in which one of Cas9’s two critical enzymatic residues has been converted to an alanine...deaminases edit A-to-G.) In short, the impaired Cas9 localizes the deaminase to where the edit is desired, and...to dCas9.  This technique has been exploited to localize factors such as transcriptional activators and...

  2. How to Write a Scientific Review Article

    Type
    Blog Post
    ... to synthesize a large volume of information: basically, a scientist’s tool kit. I wrote a review article... was cheating. Eventually I learned that it’s practical because there simply isn’t enough time to read...used both. Curate and present some useful data Typically lists and pictures are the most useful parts of...

  3. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...even when larger vector genomes are packaged, the physical size of the genomes is still ~4.7kb. But despite...overlapping strategy has a region of homology, typically ~400-1400 bp long between part A and B of the ...overlapping split vector had an improvement in muscle physiological performance.  2. Trans-splicing Overview: For...

  4. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...blocking off time for different tasks. I use google calendar for this and try to give myself flexibility by...activities that are needed to accomplish these goals. Practically speaking, this means I write everything in a ...down? It’s more viscerally appealing for me to physically cross things out (it just feels good) and I remember...

  5. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...clones from that population. You can additionally calculate the minimum number of clones you would have to...consider using a version of Cas9 that has been specifically engineered for reduced off target editing, such...Resources at Addgene: Read our CRISPRGuide Get practical help from our CRISPR Protocols Find gRNA Design...

  6. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...here). Cas9 is commonly tagged with a nuclear localization sequence to enhance targeting to the nucleus...interest (GOI) into plant cells. A) The system typically consists of a Ti plasmid, into which the GOI is...(2020) Targeted delivery of nanomaterials with chemical cargoes in plants enabled by a biorecognition ...

  7. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...used to mutate the PAM sequence (an NGG sequence critical for Cas9 cleavage), thereby rendering the resulting...unique sites and allows for the introduction of specifically designed mutations (not limited to frame shift...temperature in the polymerase chain reaction." Analytical Biochemistry 209.2 (1993): 284-290. PubMed PMID...

  8. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...vitro, and what obstacles must be overcome for clinical success. ART and HIV-1 reservoirs HIV-1 infects...these methods represent exciting advances in preclinical HIV-1 research that should be pursued in animal... field, the lessons learned in pursuing one technological application can benefit many others and accelerate...

  9. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ... incredibly important to the stability of the biological research enterprise going forward, in this post...Addgene), that need people who are data oriented, scientifically minded, and able to communicate complex information...underrepresented groups under their mentorship with the same critical eye they place on others within their labs - as...

  10. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...Using AAV vectors in complex biological systems can be tricky at best, and downright infuriating at worst...promoter in vivo. Molecular Therapy - Methods & Clinical Development, 3, 16062. https://doi.org/10.1038... Expression specificity of GFAP transgenes. Neurochemical Research, 29(11 SPEC. ISS.), 2075–2093. https...

  11. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...sequences. Non-editing CRISPR applications direct catalytically dead dCas9 fused to a variety of epigenetic ...from Addgene. For most of these constructs, catalytically dead modifiers are also available as controls...Resources on the Addgene Blog Cas9 Activators: A Practical Guide CRISPR Between the Genes: How to Experiment...

  12. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...transposition: Class I TEs, also called retrotransposons, and Class II TEs, also called DNA transposons. DNA transposons...in a genome. But there is a type of mobile DNA, called a transposon, that’s a bit hyperactive and likes...

  14. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    ...understanding of how protein folding works and creating biological tools not found in nature. Dr. Baker’s team was...was not based on any existing protein structures, called Top7. Figure 1: Top7 receives the Nobel ...

  15. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    ...of an “electricity aggregation program” for all local households and businesses. Electricity aggregation... energy expenditure here at Addgene. These are critical systems that cannot be removed or substantially...

  16. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...E6 regulatory element, which targets it to VIP cortical interneurons. Take a look to see if these new ...Wilson New serotype AAVrg = AAV retrograde (also called AAV2-retro) Looking for more viral vectors? Browse...

  17. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...preparations of several variants of a new optogenetic tool called PdCO, deposited by the Yizhar lab. Addgene is now...preparations of several variants of a new optogenetic tool called PdCO.  This is an "optoGPCR" (coupled to Go, so...

  18. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    ...with most reagents sent, and the most recent full calendar year When the website is launched, it will show...Amsterdam). He develops, characterizes, and uses genetically encoded fluorescent probes. You can follow him...

  20. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    ...wear a mask,↔️social distance, 💓show gratitude,☎️ call up an old friend. Remind them you care!#thanks #...viruses. Final tree with the complete #MicrobeAdventCalendar ornament set (plus more!) It features archaea...
Showing: 741 - 760 of 1019 results