Addgene

We narrowed to 816 results for: EED

Showing: 741 - 760 of 816 results

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Overcoming the AAV Size Limitation for CRISPR Delivery

Type

Blog Post

... for some applications, it doesn’t eliminate the need to deliver larger cargo such as base editors and...
The PAM Requirement and Expanding CRISPR Beyond SpCas9

Type

Blog Post

...requirement for Cas9-mediated DNA cleavage. The need for more PAM sequences While PAM sequences for the...
Finding nucleic acids with SHERLOCK and DETECTR

Type

Blog Post

...sensitivity and specificity without compromising speed, ease of use, and portability. This method can be...
Plasmids 101: Plasmid Incompatibility

Type

Blog Post

...replication and the replication control machinery (Need a refresher on the Origin of Replication? See our...
CRISPR 101: Targeting RNA with Cas13a (C2c2)

Type

Blog Post

...RNAs) or studying RNA processing in live cells. Indeed, groups have utilized dCas13 fusion proteins for...
Using Video to Share Your Science: We Share Science

Type

Blog Post

... about the Evolution of Scientific Impact Data Freedom: The Expansion of Data Sharing in Research Publications...
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

Type

Blog Post

...arise and tools to deactivate specific genes are needed. Targeted mutation has been demonstrated in zebrafish...
New Tools Enable CRISPRa for Neuroscience Applications

Type

Blog Post

...laborious because multiple plasmids/viruses are needed to deliver all the protein and RNA components, ...
Viral Production at Addgene

Type

Blog Post

...) containing lentivirus is first cleared by low-speed centrifugation and then by filtration through a ...
CRISPR 101: Any Base Transversion Editors

Type

Blog Post

... (T) or cytosine (C). Several different teams succeeded with this approach around the same time (He et...
Rett Syndrome: A History of Research and Therapeutic Outlooks

Type

Blog Post

...researchers can find the reagents and resources they need to cure Rett Syndrome. If you’re new to Rett Syndrome...
To Codon Optimize or Not: That is the Question

Type

Blog Post

... a protein in its native host there is likely no need to codon optimize as this gene should already be...
Tips for Using FRET in Your Experiments

Type

Blog Post

...hero (that I’m not), FRET loves playing “live.” Indeed, FRET was one of the first techniques which enabled...
Adeno-associated Viruses (AAVs) for Genome Editing

Type

Blog Post

...occasionally less than 1%, and virtually never exceeds 40%. While this is comparable or better than the...
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

Type

Blog Post

...select for desirable traits using traditional plant breeding approaches, these techniques are cumbersome, often...
Quick Guide to Near-Infrared Fluorescent Proteins

Type

Blog Post

... to help researchers choose the probe for their needs. You’ll find basic information on NIR FPs, their...
Antibodies 101: Single Chain Fragment Variables (scFvs)

Type

Blog Post

...single-chain Fv analogue produced in Escherichia coli. Proceedings of the National Academy of Sciences 85:5879–5883...
Harnessing Bacterial Toxins for Allelic Exchange

Type

Blog Post

... polylinker sequences of molecular vectors". Proceedings of the Belarusian State University Series of ...
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

Type

Blog Post

...identify two functionally distinct populations." Proceedings of the National Academy of Sciences 109.2 (2012...
CRISPR 101: Homology Directed Repair

Type

Blog Post

...molecule containing your desired edit are all you need to generate a knock-in. General considerations...

Showing: 741 - 760 of 816 results

Results per page:

Overcoming the AAV Size Limitation for CRISPR Delivery

The PAM Requirement and Expanding CRISPR Beyond SpCas9

Finding nucleic acids with SHERLOCK and DETECTR

Plasmids 101: Plasmid Incompatibility

CRISPR 101: Targeting RNA with Cas13a (C2c2)

Using Video to Share Your Science: We Share Science

Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

New Tools Enable CRISPRa for Neuroscience Applications

Viral Production at Addgene

CRISPR 101: Any Base Transversion Editors

Rett Syndrome: A History of Research and Therapeutic Outlooks

To Codon Optimize or Not: That is the Question

Tips for Using FRET in Your Experiments

Adeno-associated Viruses (AAVs) for Genome Editing

CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

Quick Guide to Near-Infrared Fluorescent Proteins

Antibodies 101: Single Chain Fragment Variables (scFvs)

Harnessing Bacterial Toxins for Allelic Exchange

Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

CRISPR 101: Homology Directed Repair

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