3D Printing Meets CRISPR Cas9
Type
Blog Post
...compelling, and I encourage you to hold one.
Why do we need physical models of protein structure?
Structural...
We narrowed to 830 results for: EED
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PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog Post...created in about a month and a half, without the need for complicated cloning of homology arms. MMEJ... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...Flexible GS linkers may also work. You will also need to generate negative control constructs — more on... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog Post...Bacteria: Table 2 also has a link to the plasmid you’ll need to express your nanobody of choice in bacteria. ... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog Post... and stability of the Cas9 protein in the host. Indeed, research has shown that a Cas9 protein from Geobacillus... -
AAVs in Retinal Gene Therapy
TypeBlog Post... systemic delivery of high doses of vectors are needed. Additionally, AAVs do not integrate in the host... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog Post...transgene into a specific cell type in the brain, you needed to make a transgenic mouse. Combining AAv.PHP.eB... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog Post...promoter sequence. Thus, T7 RNA polymerase also needs to be present in the cells to transcribe the viral... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog Post...sounds a little neurotic, but hey- sometimes fun needs to be had in an orderly and timely fashion. 9. ... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog Post...this versatile protein to suit their experimental needs, and some of the common applications in the lab.... -
Viral Production at Addgene
TypeBlog Post...) containing lentivirus is first cleared by low-speed centrifugation and then by filtration through a ... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog Post... for some applications, it doesn’t eliminate the need to deliver larger cargo such as base editors and... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog Post...requirement for Cas9-mediated DNA cleavage. The need for more PAM sequences While PAM sequences for the... -
Finding nucleic acids with SHERLOCK and DETECTR
TypeBlog Post...sensitivity and specificity without compromising speed, ease of use, and portability. This method can be... -
Plasmids 101: Plasmid Incompatibility
TypeBlog Post...replication and the replication control machinery (Need a refresher on the Origin of Replication? See our... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog Post...subsequent reprogramming success [1]. Fibroblasts need to be cultured and expanded in vitro for several... -
AAV Titers: Where Do They Come From And What Do They Mean?
TypeBlog Post... and thus are deemed reliable, or if the sample needs to be quantified again. Some of the issues with... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog Post...arise and tools to deactivate specific genes are needed. Targeted mutation has been demonstrated in zebrafish... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog Post...laborious because multiple plasmids/viruses are needed to deliver all the protein and RNA components, ... -
QC Sequencing Technologies at Addgene
TypeBlog Post...sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, 74(12), ...