We narrowed to 824 results for: EED

1. CRISPR 101: Any Base Transversion Editors

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   ... (T) or cytosine (C). Several different teams succeeded with this approach around the same time (He et...

2. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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   ...RNAs) or studying RNA processing in live cells. Indeed, groups have utilized dCas13 fusion proteins for...

3. Viral Production at Addgene

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   ...) containing lentivirus is first cleared by low-speed centrifugation and then by filtration through a ...

4. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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   ...subsequent reprogramming success [1]. Fibroblasts need to be cultured and expanded in vitro for several...

5. AAV Titers: Where Do They Come From And What Do They Mean?

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   ... and thus are deemed reliable, or if the sample needs to be quantified again. Some of the issues with...

6. Using Video to Share Your Science: We Share Science

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   ... about the Evolution of Scientific Impact Data Freedom: The Expansion of Data Sharing in Research Publications...

7. Tips for Using FRET in Your Experiments

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   ...hero (that I’m not), FRET loves playing “live.” Indeed, FRET was one of the first techniques which enabled...

8. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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   ...identify two functionally distinct populations." Proceedings of the National Academy of Sciences 109.2 (2012...

9. Quick Guide to Near-Infrared Fluorescent Proteins

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   ... to help researchers choose the probe for their needs. You’ll find basic information on NIR FPs, their...

10. Antibodies 101: Single Chain Fragment Variables (scFvs)

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    ...single-chain Fv analogue produced in Escherichia coli. Proceedings of the National Academy of Sciences 85:5879–5883...

11. Harnessing Bacterial Toxins for Allelic Exchange

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    ... polylinker sequences of molecular vectors". Proceedings of the Belarusian State University Series of ...

12. An Integrin Antibody Toolkit from IPI

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    ...tension, however, the researchers realized they needed specific tools to interpret the roles of individual...

13. CRISPR 101: Homology Directed Repair

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    ...molecule containing your desired edit are all you need to generate a knock-in.   General considerations...

14. Adeno-associated Viruses (AAVs) for Genome Editing

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    ...occasionally less than 1%, and virtually never exceeds 40%. While this is comparable or better than the...

15. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    ...select for desirable traits using traditional plant breeding approaches, these techniques are cumbersome, often...

16. Using Phosphoserine to Study Protein Phosphorylation

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    ...the ORF to be expressed Finally, the researcher needs a plasmid encoding their open reading frame of interest...

17. Plasmids 101: Common Lab E. coli Strains

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    ...plasmid. A low copy-number plasmid, encodes proteins needed for bacterial conjugation. Genes listed on F´ are...

18. Recombinase-based State Machines Enable Order-dependent Logic in vivo

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    ...-based state machines for your own experimental needs. For more context, exposition, and detail, please...

19. A Guide to Getting Started in Undergrad Research

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    ...closest friends and have often provided me with much-needed emotional support during my journey. When you are...

20. RNA Interference in Plant Biology: New Tools for an Old Favorite

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    ...and other organisms lacking this innate immunity need not worry and have benefited from the ease of use...