We narrowed to 982 results for: IGH@
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TypeBlog Post...‘foldscope’ (Dr. Prakash, Stanford University). Right there on the floor outside the lecture hall. It ...addition to low costs, deep tissue penetration, and high spatial resolution are benefits of this technique...
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Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton
TypeBlog Post...approaches. Why researchers should have a mix of high-risk, high-reward and low-risk but reliable projects. ... -
Save Time and Money by Making Your Own Competent Cells
TypeBlog Post... to make your own competent cells. If you need a high efficiency of transformation (for example, if you...However, you can skip this incubation step and go straight to plating if your transformed cells are being... -
Our Most Popular Blog Posts from 2019
TypeBlog Post...sacB counter-selection) tools used in cloning. 8. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1... Proteins 101: GFP Fusion Proteins - Making the Right Connection by Joachim Goedhart Guest blogger Joachim... -
Editor's Choice, August 2016
TypeBlog Post...experiences with CRISPR alternative, NgAgo. As you might expect with any nascent technology, researchers ...from Addgene Executive Director Joanne Kamens. I highly recommend taking a look at all of our posts as ... -
Modulate the Activity of 17 Signaling Pathways with One Kit!
TypeBlog Post...cancers are treated with drugs designed to hit them right where it hurts, the effects are often remarkable...that confer resistance to drugs,” in a rapid, straightforward, and scalable manner, Wood said. Thankfully... -
From our Table to Yours: An Inside Look at Lunch at Addgene
TypeBlog Post...Right from the start, when we founded Addgene nearly 10 years ago, lunch felt like a family meal. My ... we have a few unique lunchtime traditions that might be fun to incorporate into your own lab. Iron Chef-style... -
X-CHIME: Context Dependent Germline Knockout in Immune Cells
TypeBlog Post...systems enable gene knockout in immune cells in a high-throughput manner, but these systems have not been...also includes pXPR_071, a plasmid that leads to higher titer lentivirus preparations than the first generation... -
Searchable and Sortable gRNAs for Your Next CRISPR Experiment
TypeBlog Post...what you're looking for. Search using the box highlighted in red above. When searching, it is worth trying... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog Post...precise genome edits, the recombinases used must have high activity and specificity with low cross-reactivity...recombinase/reporter pairs were inactive, even at very high recombinase levels. These data show that multiple... -
London Calling - Addgene’s New Europe Office in London
TypeBlog Post...collaborators was extremely helpful, but with hindsight I wished I had gotten them from Addgene. I really...really struggled to get those plasmids with the right information and I believe that a lot of European ... -
Fluorescent Proteins 101: Fluorescent Biosensors
TypeBlog Post...signal. Reporter signals may be electrochemical or light-based, with luminescent and fluorescent reporters...transfer between the fluorescent proteins. FRET is highly amenable to biosensor creation, and custom FRET... -
Antibodies 101: Affinity Tags
TypeBlog Post...proteins from E. coli is generally more straightforward and of a higher purity than mammalian purifications... -
Early Career Researcher Toolbox: Free Online Molecular Biology Tools
TypeBlog Post...mapping and design (Figure 1). Benchling: While you might think of Benchling as an electronic lab notebook...Design (DAD): This tool designs overhang sets for high complexity Golden Gate Reactions Cloning ligation... -
New Viral Vectors - Fall 2024
TypeBlog Post...monkeys it actually targets neurons, and has 45-fold higher expression levels compared to AAV9. You can now...AAV-CAG-GFP packaged in AAV9-X1.1! We also have eight new AAV-PHP.eB vectors from the Jonathan Ting lab... -
The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place
TypeBlog Post...growing open repository of CRISPR screen data that might be able to help you out. What is the Open Repository... cross-interrogation of different datasets that might otherwise be hard to identify. Figure 2: ... -
Oh, The Places You Can Go: Careers in Science Communication - Science Writing
TypeBlog Post...about science communication careers, you’re in the right place. For this series, I’ll interview three science... a writing position and looking for someone who might take over. I like writing and science, so I took... -
Tips for Improving Your Next Manuscript
TypeBlog Post...leaders in the field of microbiology with multiple high-profile publications. My classmates consisted of...scientific manuscript is not prose; it is to be straight to the point. 3. Engage the reader Scientists ... -
The Blue Flame Award: Celebrating Addgene's Most Requested Depositors
TypeBlog Post...celebrate our depositing PI’s who have the most highly requested plasmids. Currently, there are roughbly... highly requested plasmids... -
The Stingy Scientist: How the Baby Gel Box Was Born
TypeBlog Post... I settled on using glass slides. With just the right amount of agarose, I could pour a very thin gel ...surface tension. It's a bit tricky to pour the right amount so the agarose doesn’t pour over the sides...