We narrowed to 978 results for: LARS
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TypeBlog Post... nm will pass through animal and human tissues largely unimpeded. The challenge then was to develop fluorescent... Ultrasound wavelengths, on the other hand, are larger than the size of a cell, so they scatter much less...
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Hot Plasmids and Viral Preps - July 2021
TypeBlog Post...internal control and a SL-sensitive fluorophore (circularly permuted green fluorescent protein or cpGFP) ... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection... -
What's the Best Way to Elute and Store Your Plasmid DNA?
TypeBlog Post...essential cofactors for many tools used in modern molecular biology like restriction enzymes and polymerases...posts Resources on Addgene.org Check out the Molecular Biology Reference Find protocols on plasmid cloning... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog Post..., as well as to optogenetically control the depolarization of R2 neurons using CsChrimson::tdTomato, an...fluorescent protein plasmids at Addgene Read our Molecular Biology Reference ... -
CRISPR 101: Cas9 Nickase Design and Homology Directed Repair
TypeBlog Post... small deletions, while H840A is biased towards large insertions. These profiles are likely due to the... Oligonucleotides) Use 100 nt homology arms for large insertions (long ssDNA; IDT Megamer® ssDNA Fragments... -
Antibodies 101: Affinity Tags
TypeBlog Post...purification, it will be expressed along with all other cellular proteins within the expression system (bacteria...protein) with amylose resin. The MBP tag, although large, can actually increase expression and solubility... -
FastCloning: A Newer, Simpler Cloning Technique
TypeBlog Post...Protein Science at the University of Connecticut. Molecular cloning is the isolation, insertion and amplification... recombinant DNA without sequence alteration. Molecular cloning techniques are some of the most widely... -
An Addgene Summer Internship
TypeBlog Post...day at Addgene last summer, through the La Vida Scholars summer program. I was unsure whether or not I ...which was the impression I got from the La Vida Scholars tour. After some time had passed, I reached out... -
Oh, The Places You Can Go: Careers in Science Communication - Science Writing
TypeBlog Post...writer When Hans was finishing up his PhD in Molecular Neuroscience at the University of Iowa, he was...over hosting them. Since then, webinars make up a larger part of Hans’ workload. “I am currently getting... -
Some Like it Hot: Thermostable GeoCas9
TypeBlog Post...overlapping PAM. In both cases, GeoCas9 and SpyCas had similar levels of editing. See figure 4 in Harrington et...thermostability could also be used with in vitro molecular bio applications that require high temperature... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog Post... not seem much different than my second son of similar age. When he came to visit us and watch fellow ...jarring of his body could initiate very painful FOP flare ups. Eventually, without a cure, AJ will begin losing... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog Post... or p19 promoters to, respectively, produce the large (Rep78 and Rep68) and small (Rep52 and Rep40) Rep...Targeting and Improving the Efficacy of AAV Vectors. Molecular Therapy - Methods & Clinical Development 12:248... -
Management for Scientists: Giving Feedback
TypeBlog Post...style. Formalizing the feedback session Giving regular, informal feedback is a very effective way to bring...employee. This is especially true if the problems are large enough that the employee should be aware that they... -
Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio
TypeBlog Post...attacks. He described one such recent attack as "hilarious". Welcome to Montana! Though sadly I will not ...components, and challenging target genomes, such as the malaria-causing Plasmodium falciparum. Throughout these... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog Post...Resistance to antibiotics is a widely used tool in molecular biology, yet scientists rarely stop to think about...Plasmid Pages Resources on Addgene.org Browse Our Molecular Biology Reference Pages View our Plasmid Protocols... -
Intro to Our Summer SciComm Series!
TypeBlog Post... all be defined as laypeople when it comes to molecular biology. Some lay people have a high amount of...more likely to closely read an email sent out to a large listserv or one sent to you and a few other people... -
RUBY-Red Siliques
TypeBlog Post...equipment. Reporters for studying gene expression have similar drawbacks, often requiring the destruction of plants... for GUS staining or are not suited to studying large samples. RUBY Red But recently, something new has... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog Post...referred to as TX-TL systems) have been gaining popularity as a synthetic biology tool. These systems allow... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog Post...resistant to vaccine serum responses. This result was similar to studies that used live virus (Cele et al., 2021...neutralization rates of B.1.351 pseudovirus was similar to that of pseudoviruses representing a bat-derived... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog Post...deposited from Di Ventura's lab, including the popular light-inducible nuclear export system (LEXY) here...page Resources on Addgene.org Check out the Molecular Biology Reference Download Addgene's Plasmids ...