We narrowed to 971 results for: LARS
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TypeBlog Post...internal control and a SL-sensitive fluorophore (circularly permuted green fluorescent protein or cpGFP) ... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection...
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What's the Best Way to Elute and Store Your Plasmid DNA?
TypeBlog Post...essential cofactors for many tools used in modern molecular biology like restriction enzymes and polymerases...posts Resources on Addgene.org Check out the Molecular Biology Reference Find protocols on plasmid cloning... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog Post..., as well as to optogenetically control the depolarization of R2 neurons using CsChrimson::tdTomato, an...fluorescent protein plasmids at Addgene Read our Molecular Biology Reference ... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog Post... or p19 promoters to, respectively, produce the large (Rep78 and Rep68) and small (Rep52 and Rep40) Rep...Targeting and Improving the Efficacy of AAV Vectors. Molecular Therapy - Methods & Clinical Development 12:248... -
Some Like it Hot: Thermostable GeoCas9
TypeBlog Post...overlapping PAM. In both cases, GeoCas9 and SpyCas had similar levels of editing. See figure 4 in Harrington et...thermostability could also be used with in vitro molecular bio applications that require high temperature... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog Post... not seem much different than my second son of similar age. When he came to visit us and watch fellow ...jarring of his body could initiate very painful FOP flare ups. Eventually, without a cure, AJ will begin losing... -
Management for Scientists: Giving Feedback
TypeBlog Post...style. Formalizing the feedback session Giving regular, informal feedback is a very effective way to bring...employee. This is especially true if the problems are large enough that the employee should be aware that they... -
Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio
TypeBlog Post...attacks. He described one such recent attack as "hilarious". Welcome to Montana! Though sadly I will not ...components, and challenging target genomes, such as the malaria-causing Plasmodium falciparum. Throughout these... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog Post...Resistance to antibiotics is a widely used tool in molecular biology, yet scientists rarely stop to think about...Plasmid Pages Resources on Addgene.org Browse Our Molecular Biology Reference Pages View our Plasmid Protocols... -
Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC
TypeBlog Post...challenge in the best of cases. But it can be particularly challenging when your only antibody option is...al., 2013). One issue with this strategy is that large complexes may not permeate through your tissue samples... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog Post...This is, for the intrepid researcher looking for molecular biology tools, a very useful trait. Virus structure... Viral vectors Viruses are quite appealing as molecular biology tools… if one isn’t too bothered by the... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog Post...Shen, 2011). To our knowledge the effect of hyperosmolarity on AAV production yields had yet to be examined...., and Bernhard O. Palsson. "Effect of medium osmolarity on hybridoma growth, metabolism, and antibody... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog Post...referred to as TX-TL systems) have been gaining popularity as a synthetic biology tool. These systems allow... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog Post...resistant to vaccine serum responses. This result was similar to studies that used live virus (Cele et al., 2021...neutralization rates of B.1.351 pseudovirus was similar to that of pseudoviruses representing a bat-derived... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog Post...deposited from Di Ventura's lab, including the popular light-inducible nuclear export system (LEXY) here...page Resources on Addgene.org Check out the Molecular Biology Reference Download Addgene's Plasmids ... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog Post...protein phosphatase that responds to rising intracellular calcium levels within the cell. When levels ... wild type (top panels) and cells with high intracellular Ca2+ (bottom panels) equipped with destabilized... -
Plasmids 101: Origin of Replication
TypeBlog Post...maintain fewer copies of plasmids if they contain large inserts or genes that create a toxic product. ...die off. Have any questions? Need something clarified? If so please, post in the comments. References... -
Intro to Our Summer SciComm Series!
TypeBlog Post... all be defined as laypeople when it comes to molecular biology. Some lay people have a high amount of...more likely to closely read an email sent out to a large listserv or one sent to you and a few other people... -
RUBY-Red Siliques
TypeBlog Post...equipment. Reporters for studying gene expression have similar drawbacks, often requiring the destruction of plants... for GUS staining or are not suited to studying large samples. RUBY Red But recently, something new has... -
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools
TypeBlog Post... are still just as specific to their target as regular antibodies. These properties make nanobodies easier...Wanisch Listen to the C-BERST podcast segment Similarly to the Carr lab, Erik Sontheimer and his group...