We narrowed to 974 results for: LAT
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TypeBlog Post...when Gal4 is expressed. Gal4 expression can be regulated by any chosen promoter, and Bloomington’s Stock...make your own. In my next post, I’ll explain the relatively straightforward process of generating your own...
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Four Factors that Differentiate the Stem Cell Field
TypeBlog Post...that stem cell scientists are so focused on translational research is encouraging. Patients are key to...depositors Marius Wernig, Kristin Baldwin, Kathrin Plath, Hans Schöler, and closing keynote speaker Rudolph... -
Optimizing Donor DNA for Enhanced CRISPR Genome Editing
TypeBlog Post...process in detail often suggests new strategies to manipulate that process. We began our research with a simple...approaches. Moreover, the cost of reagents is relatively modest, requiring expressed Cas9 protein, transcribed... -
Celebrate National Book Month with Addgene
TypeBlog Post...Sacks "Neurologist Oliver Sacks examines the relationship between the human brain and music: how we perceive...roles microbes play in our ecosystem, examining relationships between microbes and insects, mammals, and plants... -
New and Upcoming Viral Vectors - June 2019
TypeBlog Post...provides improved detection for tracking large populations of neurons in wide-field imaging (Dana et al.... intersectional control of specific neuronal populations. We now offer a Flp-expressing AAV, as well as... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog Post...the same myths and misconceptions fifteen years later. What does that look like? One of my more recent...designs could work to slow the spread of illness. I later helped run workshops where I showed people how they... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog Post...trigger other forms of neuronal signaling and regulation. To create iGluSnFR, the Looger lab wedged GFP...soluble protein CAG: Generic strong promoter. Formulated as AAV-DJ for neuronal expression. CAG-FLEX: ... -
Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog Post...is a challenge. To help keep you abreast of the latest fluorescent proteins, I maintain an interactive... is sufficient to use a spectrum for a closely related protein, if you know that your protein of interest... -
Advice for Scientists Starting a Lab
TypeBlog Post...pub with us in the evening, wanting to know our latest results as soon as the gels ran.” Dr. Ellis tries...emphasis on genetic engineering, sequencing, and translation of results to companies.” Take home message Starting... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog Post... determine the implications of your genetic manipulations. You can produce some very beautiful immunofluorescent...model organism is in its amenability to genetic manipulation. This is just a primer so that you are aware... -
Luminescent Imaging with Nano-lanterns
TypeBlog Post...continuously imaged cells for about 4 hours! The latest enhanced Nano-lanterns (eNL) described in Suzuki...signals of various Nano-lanterns, but it does not isolate each Nano-lantern, so Takai et al. used a linear... -
Plasmids 101: Screening Strategies Used in Plasmid Cloning
TypeBlog Post...information (more on this below). Plating the transformation on ampicillin plates containing X-gal allows you... -
New and Upcoming Viral Vectors - May 2020
TypeBlog Post...offered by the system that allows for specific manipulation of neurons. We have expanded our AAV collection...Chemogenetics offers noninvasive methods for manipulating cell activity, particularly in the brain, and... -
Antibodies 101: Isotypes
TypeBlog Post...region length. Now for some essential isotype nomenclature. Antibody heavy chain proteins as well as the...start by co-expressing IgM and IgD antibodies, but later in an immune response, the variable region of an... -
The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It
TypeBlog Post...more credible than they actually are. Throwing out plates that don’t respond as expected, use of representative...different results. Different setting, different population, new genetic variation, or different handling... -
Behind the scenes: Addgene’s new search engine and more
TypeBlog Post...adding more terms. Weighting We determined the relative importance of different bits of information for...shortcuts to specific catalog items as well as related items (for example, a kit that contains a plasmid... -
Dos & Don'ts When Publishing a Scientific Manuscript
TypeBlog Post...? Sometimes, the funders of your research may stipulate that your article must be published in an open...data behind the figures and results. This is a relatively new development, and it allows reviewers and ... -
dTAG - You're it!
TypeBlog Post...FKBP12F36V-tagged proteins using our lentiviral expression platform, to assess selective degradation of FKBP12F36V-BRD4...dTAG-47 Weintraub et al. YY1 Is a Structural Regulator of Enhancer-Promoter Loops. Cell. December 14 ... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog Post...always easy to track at the transcriptional or translational level. However, several genetic tools have been.... These allow for not only recording but also modulating bioelectric states in vivo. For example, the ... -
Antibodies 101: Affinity Reagents
TypeBlog Post...antibodies to antibody fragments to tools completely unrelated to antibodies. Why use an alternative affinity... These are antibody mimics and are not closely related to antibodies. Instead, they are derived from a...