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  1. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...trigger other forms of neuronal signaling and regulation. To create iGluSnFR, the Looger lab wedged GFP...soluble protein CAG: Generic strong promoter. Formulated as AAV-DJ for neuronal expression. CAG-FLEX: ...

  2. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...DNA – perfect for transforma­­­tion or as a PCR template. Some of the applications of the NEBuilder HiFi...plasmids can be directly transformed into E.coli for plating, screening and generation of sgRNA. Generation ...

  4. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...the same myths and misconceptions fifteen years later. What does that look like?  One of my more recent...designs could work to slow the spread of illness. I later helped run workshops where I showed people how they...

  5. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...that stem cell scientists are so focused on translational research is encouraging. Patients are key to...depositors Marius Wernig, Kristin Baldwin, Kathrin Plath, Hans Schöler, and closing keynote speaker Rudolph...

  6. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...process in detail often suggests new strategies to manipulate that process. We began our research with a simple...approaches. Moreover, the cost of reagents is relatively modest, requiring expressed Cas9 protein, transcribed...

  7. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...is a challenge. To help keep you abreast of the latest fluorescent proteins, I maintain an interactive... is sufficient to use a spectrum for a closely related protein, if you know that your protein of interest...

  8. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...pub with us in the evening, wanting to know our latest results as soon as the gels ran.” Dr. Ellis tries...emphasis on genetic engineering, sequencing, and translation of results to companies.” Take home message Starting...

  10. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...offered by the system that allows for specific manipulation of neurons. We have expanded our AAV collection...Chemogenetics offers noninvasive methods for manipulating cell activity, particularly in the brain, and...

  12. Antibodies 101: Isotypes

    Type
    Blog Post
    ...region length. Now for some essential isotype nomenclature. Antibody heavy chain proteins as well as the...start by co-expressing IgM and IgD antibodies, but later in an immune response, the variable region of an...

  14. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...continuously imaged cells for about 4 hours! The latest enhanced Nano-lanterns (eNL) described in Suzuki...signals of various Nano-lanterns, but it does not isolate each Nano-lantern, so Takai et al. used a linear...

  15. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...adding more terms.  Weighting We determined the relative importance of different bits of information for...shortcuts to specific catalog items as well as related items (for example, a kit that contains a plasmid...

  16. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...? Sometimes, the funders of your research may stipulate that your article must be published in an open...data behind the figures and results. This is a relatively new development, and it allows reviewers and ...

  17. dTAG - You're it!

    Type
    Blog Post
    ...FKBP12F36V-tagged proteins using our lentiviral expression platform, to assess selective degradation of FKBP12F36V-BRD4...dTAG-47 Weintraub et al. YY1 Is a Structural Regulator of Enhancer-Promoter Loops. Cell. December 14 ...

  18. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...antibodies to antibody fragments to tools completely unrelated to antibodies.  Why use an alternative affinity... These are antibody mimics and are not closely related to antibodies. Instead, they are derived from a...

  19. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...the requirements of the specific assay. For the latter three, secondary antibodies are indicated when ...absolute quantitative analysis of a sample. Instead, relative quantification can be done using either the direct...

  20. Addgene: The First Twenty Years

    Type
    Blog Post
    ...the Neuroscience AntiBody Open Resource (NABOR) platform, supported by an NIH BRAIN Initiative. We also...shipped out our two millionth item, and had a cumulative 120,000 citations of individual items in our ...
Showing: 741 - 760 of 970 results