We narrowed to 816 results for: MAL
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TypeBlog Post...titrating each lot of AAV in vivo to determine the optimal dosage. At Addgene, we don’t reserve lots, but ...
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Career Insights: Technical Support Specialist
TypeBlog Post...also the proposed salary range) should help. In smaller companies, technical support is often covered by... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog Post...website, we’ve learned about the importance of smaller, systemic changes, like using alt-text, captions... -
Tips for Writing a Good Cover Letter
TypeBlog Post... preparation for updating this post, I took an informal poll of Addgene hiring managers. All of them stated... -
Finding nucleic acids with SHERLOCK and DETECTR
TypeBlog Post...sensitivity, the DNA is first amplified through isothermal amplification by RPA. When Cas12a-cRNA base pairs... -
Lab to Office Culture Shock
TypeBlog Post...work environments outside of academia provide a formal training regime and the right set of tools to help... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog Post...-Kulka, and Gad Glaser. "An Escherichia coli chromosomal" addiction module" regulated by guanosine [corrected... -
How to Be an Excellent Trainee
TypeBlog Post...demonstrating it. Requests like this are both very normal and very helpful; don’t be afraid to ask! Especially... -
Transferable Skills Guide: Creativity
TypeBlog Post...PhDs found that many researchers feel they lack formal training in a variety of transferable skills. At... -
Hot Plasmids - November 2023
TypeBlog Post...transcriptase (RT) variants, PE6a and PE6b are 500–800 bp smaller than previous editors, but just as effective and... -
Healthcare Consulting: A Door to the Business of Life Sciences
TypeBlog Post...complex challenges, quickly breaking them up into smaller achievable tasks, and then executing on them in... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog Post...Schematic representation of integration process and episomal forms of non-integrating transgenes. Created with... -
Transferable Skills Guide: Conflict Resolution
TypeBlog Post...Substantive conflict occurs when people or teams who normally get along well with each other disagree about ... -
Plasmids 101: Restriction Cloning
TypeBlog Post...fragments of linearized DNA migrate slower than smaller linearized fragments. You can separate your backbone... -
Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog Post...nominations for government fellowships. The closing and formal evaluation of the pilot program IMP 2014/2015, ... -
A Guide to Getting Started in Undergrad Research
TypeBlog Post...yourself and the world around you, it’s perfectly normal to realize that you have new interests and that... -
Sequencing Options for CRISPR Genotyping
TypeBlog Post...Cell-based (in vitro) Can be used on tissue from whole animal models, no exogenous component required (e.g. dsODN... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog Post...show efficient absorption at 650 nm, APC-Cy7's optimal excitation happens at higher wavelengths of about... -
Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs
TypeBlog Post...management consulting firms), sponsor more H-1Bs than small startups and firms with a non-international focus... -
CRISPR Guide
TypeGuide...Browse Plasmids: Anti-CRISPR Small Precision Edits CRISPR can be used to make small, very precise edits of ...double-stranded DNA plasmid. Small ssDNA oligos often work well for small edits like a single-point mutation...Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening Cas9 Fusion...missing sequence, and is more commonly used for small precision edits. NHEJ directly ligates the break...efficient in repairing DSB, it frequently causes small nucleotide insertions or deletions (indels) at the...modification ). In most cases, NHEJ gives rise to small indels in the target DNA that result in amino acid... handful of bases, or even a single base. These small precision edits can be done via HDR, base editing...