We narrowed to 775 results for: Mos

... plasmids all replicate in E. coli, which is the most commonly used bacteria in lab manipulations. Table...

...first try to produce GVs in their own lab using the most reliable method, which is the transient cotransfection...

...to press, as there is so much analysis possible. Most scientists are, however, privileged in having exclusive...

... need to sequence your PCR to visualize an edit. Most dual guide systems generate a deletion large enough...

...mammalian, yeast, plant, and insect cells, they are most often expressed from a plasmid in bacteria (Ahmed...

...different area of the cell than CFP and YFP. The most recent derivation of Brainbow, Brainbow 3.0 (Cai...

...research. Still a Grad Student? Get Tips on Getting the Most out of the Process Get more tips from the full eBook...

...the orientation of the BbcVI restrict site matter? Most of the time no, but there are 2 instances where ...

...can be difficult to compare their performance, as most translation initiation sequences are optimised for...

...Consequently, site directed mutagenesis is one of the most popular approaches for introducing minor modifications...

...recombination, discovered in the 1980s, is one of the most important ways to spatially and temporally control...

...epigenetic modifiers available from Addgene. For most of these constructs, catalytically dead modifiers...

...manufacturer’s instructions for your competent cells. For most standard cloning, you can transform 1-2μl of your...

...traction in recent years. The goal is to engineer the most miniscule parts of life, like proteins, to study...

...fluorescent protein. FRET can be measured by exciting the most blue-shifted fluorescent protein (donor) and measuring...

...microscopy must be used. To learn more about the most suitable fluorescent microscopy technique for your...

...expressing Cre. The result of a system like this is a mosaic of cells labelled with different fluorescent proteins...

...Rather than reprogram cells all the way back to their most primitive pluripotent stem cell state, through transdifferentiation...

...photoactive Cas9 tool, as shown in the figure below. Their most successful design utilized Magnet photoswitchable...

...four adenine base editors (ABEs). ABE7.10 is the most active editor, displaying an average editing efficiency...