We narrowed to 765 results for: Mos

1. Transferable Skills Guide: Creativity

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   Blog Post

   ... tendency of a group of people to agree with the most prominent idea, and instead building an environment...

2. Hot Plasmids - November 2023

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   ...undesired edits (indels, etc.), while PE6c and PE6d were most efficient at complex edits, such as long or highly...

3. Viral Production at Addgene

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   Blog Post

   ...This article was written by multiple Addgenies and most recently updated by Ina Ersing in August 2023. ...

4. Finding nucleic acids with SHERLOCK and DETECTR

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   ...type of readout allows for nucleic acid detection almost anywhere as lateral flow strips are easy to transport...

5. Plasmids 101: Plasmid Incompatibility

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   ...same replication control machinery within the cell. Most plasmids encode a negative regulation system involving...

6. An Inside Look into Addgene's Dev Team

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   ... third party libraries and any security updates. Most of the time, updating these libraries is straight...

7. CRISPR Activation: A Practical Guide

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   Blog Post

   ...the scientific world by storm. While Cas9 has been most widely used to create specific edits in DNA, there...

8. Data Freedom: The Expansion of Data Sharing in Research Publications

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   Blog Post

   ...to press, as there is so much analysis possible. Most scientists are, however, privileged in having exclusive...

9. CRISPR 101: Validating Your Genome Edit

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   ... need to sequence your PCR to visualize an edit. Most dual guide systems generate a deletion large enough...

10. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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    Blog Post

    ...different area of the cell than CFP and YFP. The most recent derivation of Brainbow, Brainbow 3.0 (Cai...

11. Antibodies 101: Single Chain Fragment Variables (scFvs)

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    ...mammalian, yeast, plant, and insect cells, they are most often expressed from a plasmid in bacteria (Ahmed...

12. Tips for Getting a Faculty Position

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    Blog Post

    ...research. Still a Grad Student? Get Tips on Getting the Most out of the Process Get more tips from the full eBook...

13. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    Blog Post

    ...the orientation of the BbcVI restrict site matter? Most of the time no, but there are 2 instances where ...

14. Multiple Plasmids at a Low Price: Kits from Addgene

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    Blog Post

    ...can be difficult to compare their performance, as most translation initiation sequences are optimised for...

15. Site Directed Mutagenesis by PCR

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    ...Consequently, site directed mutagenesis is one of the most popular approaches for introducing minor modifications...

16. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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    Blog Post

    ...recombination, discovered in the 1980s, is one of the most important ways to spatially and temporally control...

17. Technologies Enabled by NanoLuc® Luciferase

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    Blog Post

    ...fluorescent protein. FRET can be measured by exciting the most blue-shifted fluorescent protein (donor) and measuring...

18. CRISPR 101: Epigenetics and Editing the Epigenome

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    ...epigenetic modifiers available from Addgene. For most of these constructs, catalytically dead modifiers...

19. Plasmids 101: Restriction Cloning

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    Blog Post

    ...manufacturer’s instructions for your competent cells. For most standard cloning, you can transform 1-2μl of your...

20. Antibodies 101: Flow Cytometry

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    Blog Post

    ...microscopy must be used. To learn more about the most suitable fluorescent microscopy technique for your...