We narrowed to 772 results for: Mos

1. Finding nucleic acids with SHERLOCK and DETECTR

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   Blog Post

   ...type of readout allows for nucleic acid detection almost anywhere as lateral flow strips are easy to transport...

2. Plasmids 101: Plasmid Incompatibility

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   ...same replication control machinery within the cell. Most plasmids encode a negative regulation system involving...

3. An Inside Look into Addgene's Dev Team

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   ... third party libraries and any security updates. Most of the time, updating these libraries is straight...

4. Transferable Skills Guide: Creativity

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   ... tendency of a group of people to agree with the most prominent idea, and instead building an environment...

5. Analyzing CRISPR Editing Results with ICE from Synthego

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   ...multiplex sample, the cut site will be aligned to the most 5’ cut site.     Indel Distribution In the Indel...

6. Transferable Skills Guide: External Collaborations

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   ...relationships. Personally, I find this to be the most challenging and time-intensive part of relationship...

7. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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   ...high concentration of FLEx vectors guarantees that most cells are transfected with all labeling plasmids...

8. Deciphering the Mysteries of Behavior with Viral Vectors

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   ...studies have been essential for this task. However, most lesioning methods destroy areas larger than intended...

9. Save Time with Transient Plant Leaf Transformations

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   Blog Post

   ...tissue (see Figure 3). This method is probably the most scalable method. Transfer your plant into the dark...

10. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    ...compatible PAM at your site of interest, that is the most efficient option. If there is no NGG in your genomic...

11. CRISPR Activation: A Practical Guide

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    ...the scientific world by storm. While Cas9 has been most widely used to create specific edits in DNA, there...

12. Data Freedom: The Expansion of Data Sharing in Research Publications

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    ...to press, as there is so much analysis possible. Most scientists are, however, privileged in having exclusive...

13. Tips for Getting a Faculty Position

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    ...research. Still a Grad Student? Get Tips on Getting the Most out of the Process Get more tips from the full eBook...

14. Antibodies 101: Single Chain Fragment Variables (scFvs)

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    Blog Post

    ...mammalian, yeast, plant, and insect cells, they are most often expressed from a plasmid in bacteria (Ahmed...

15. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    Blog Post

    ...the orientation of the BbcVI restrict site matter? Most of the time no, but there are 2 instances where ...

16. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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    ...different area of the cell than CFP and YFP. The most recent derivation of Brainbow, Brainbow 3.0 (Cai...

17. CRISPR 101: Validating Your Genome Edit

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    ... need to sequence your PCR to visualize an edit. Most dual guide systems generate a deletion large enough...

18. Multiple Plasmids at a Low Price: Kits from Addgene

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    ...can be difficult to compare their performance, as most translation initiation sequences are optimised for...

19. Site Directed Mutagenesis by PCR

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    Blog Post

    ...Consequently, site directed mutagenesis is one of the most popular approaches for introducing minor modifications...

20. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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    Blog Post

    ...recombination, discovered in the 1980s, is one of the most important ways to spatially and temporally control...