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  2. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...procedure (illustrated in the YouTube video below) is simple: After obtaining antibiotic-resistant colonies,...initially, or using secondary biological screens. For example, for cancer researchers, if growth properties in...

  4. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...attention on social media. I gained a concrete example to use to promote future attempts and learned a...roles of scientists outside of academia One recent example: Don't call me a dropout: Why science needs more...

  5. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ... table below lists the four common 2A peptides employed by scientists. 2A cleavage is universal in eukaryotic...reporter cloned in. In these plasmids you would simply clone your gene of interest into the multiple cloning...

  6. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...low RNA editing. To increase A->G editing, they employed hyperactive ADAR constructs, like ADAR2DD(E488Q...ADAR will preferentially edit an adenine if the template has a cytosine mismatch at that position. PspCas13b-ADAR2DD...

  7. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...correct genetic conditions over 25 years ago when completing her PhD and medical training in Ophthalmology... kb cannot be delivered using this vector. For example, although researchers know that Usher Syndrome ...

  8. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...relied on deaminase enzymes, either to directly accomplish the base edit or to create the substrate for ...to repair the DNA using the edited strand as a template — in this case, encouraging it to incorporate ...

  9. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ... solve an issue specific to that country. ‌For example, when we began offering temperature-sensitive materials...inclusive design in our digital content. We have implemented proactive measures to find potential barriers...

  10. How to Be an Excellent Trainee

    Type
    Blog Post
    ...experience In research there is a saying that if the sample prep is poor, then the data will be poor. The same...you remember where the equipment, reagents, and samples are. Do: Ask your bench mentor if they have time...

  11. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ... understand the process, we’ll walk through an example experiment where we might use Gateway cloning to...into destination vectors. The cloning process is simple -  no restriction, ligation or gel purification...

  12. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...avoid rousing the trolls. 3. The AMA process was simple and well organized. You can check out the r/Science...posters (this advice included leaving out the term “implicit bias” which is apparently a troll trigger phrase...

  17. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ... purified from the system, researchers can run samples on SDS-PAGE gels containing Phos-Tag, a small molecule...substitution. Alternatively, you can express the completely wild type protein from the same plasmid.  Upon...

  18. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...regularly. Dystrophin and genome editing Since complex oligonucleotide treatment comes with many challenges...therapy, it’s important to realize that DMD is a simpler case than other genetic diseases we’d like to treat...

  19. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...specific list of the candidate off-target sites. For example, if there are 10 putative off-target locations ...confounding your results should be minimal. For example, if your goal is to generate a knock-out cell line...

  20. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...emotions and assumptions. I’m not just talking about simple things like “Which route should I take to get to...choosing a training lab, I strongly suggest you complete a work rotation in the lab if at all possible....
Showing: 741 - 760 of 930 results