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We narrowed to 1,020 results for: NTS;

Showing: 741 - 760 of 1020 results

  1. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...relationships between microbes and insects, mammals, and plants, among others. I particularly enjoyed the emphasis... their paths cross with each other and with the events of the times."     - Aliyah Weinstein, Marketing...community know what your favorite book is in the comments! Additional resources on the Addgene blog Learn...

  2. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...Recombinases And more! Control AAV For many AAV experiments, we recommend validating the serotype in your...vectors to more serotypes. We currently package four variants of these biosensors, which are optimized for specific... should improve the specificity of influence measurements by reducing photostimulation of the axons and...

  4. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...Darrell Kotton 2. Stem cell scientists care about patients As any grad student who has spent years studying...: Dr. Yamanaka talked about his clinical trial patients with advanced macular degeneration; Laurenz Studer...focused on translational research is encouraging. Patients are key to the research, but they are also the...

  5. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    ...) and are ready to dive into your functional experiments! Whether you’ve cloned the plasmid yourself or...determine the relative sizes of the resulting DNA fragments. Restriction digests are commonly used to confirm...longer to achieve good separation of larger DNA fragments. I hope these tips demonstrate that plasmid ...

  6. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ... and you’ll need to track it throughout your experiments.  Now that that’s out of the way, you can put... reaction to understand if any of your buffer components could affect the reaction. For instance, sodium... - antibodies that are a good match for your experiments, and avoid the awkwardness of getting the right...

  7. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...they successfully imaged multiple subcellular compartments over several minutes using Nano-lantern fusion...single molecule imaging. Nano-lanterns and your experiments The Nagai laboratory has a done a wonderful job...Addgene. Suzuki, Kazushi, et al. “Five colour variants of bright luminescent protein for real-time multicolour...

  8. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...the N and C terminal segments respectively of a GFP dependent Cre. The two segments come together in the...transduction patterns empirically for their own experiments. To this end, we offer control AAV in smaller...

  9. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...being depicted, we can’t forget that those other components are still there in real life.  Certainly referring...specifically study it. They might be researching enhancer elements that control the transcription of a gene a megabase...versatile, small, sensitive immunohistochemical reagents. Yamagata M, Sanes JR Proc Natl Acad Sci U S A...

  10. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    ...skills, while also learning about new scientific developments and helping scientists is a win-win for me. ...shared in the post. And the blog writing tips are elements I try to include in each post I write for the ...your research or career? Share your story in the comments below!   Additional resources on the Addgene...

  11. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    ...idea that would evolve into Sample6 as graduate students in Dr. Jim Collin’s Lab in 2009. From 2009 to ... an idea or product? Please let us know in the comments section! And read on for more great information...contributed by Julia Phillips and Fiona Beckman Acknowledgments Thank you to Dr. Mike Koeris (Co-founder and...

  12. To Each HIS Own

    Type
    Blog Post
    ...wash steps removes excess proteins and other contaminants. The protein of interest is then eluted using...polyhistidine-metal ion interaction is not disrupted by denaturants (Hochuli, 1988). In some cases, proteins can ...more stringent wash conditions and remove more contaminants without losing your protein of interest. But...

  13. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...Addgene, so troubleshooting molecular biology experiments was in the job description, but this skill is...broader research questions and designing future experiments. Being able to work through these types of bigger....     Know your strengths! Research experience presents ample opportunities to practice problem solving...

  14. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...CRISPR-Cas systems SEND plasmids Prime editing improvements Miniature CRISPR-Cas systems By Brook Pyhtila... from Segel et al. 2021.   Prime editing improvements By Angela Holmes Several labs have recently ...PEDAR is capable of correcting larger genomic rearrangements as demonstrated in a mouse model of tyrosinemia...

  16. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ... aggregate. The basics and pitfalls of these experiments are discussed here. In vitro tests for aggregation...your cell type of interest before starting your experiments.  Selecting a non-oligomerizing protein A fluorescent... Of course, you want the ‘latest and greatest’ variants with the best properties for your research. But...

  17. RNA Extraction Without A Kit

    Type
    Blog Post
    ...such as RNaseZAP. When harvesting tissues, cells, plants, fungi, or bacteria, keep samples cold and work...the homogenate was loaded onto cesium-chloride gradients and ultracentrifuged for up to 20 hours to separate...instead of RNA separation using cesium-chloride gradients, water-saturated phenol, sodium acetate, and chloroform...

  18. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...restriction sites to easily assemble multiple DNA fragments. This system has been used to construct plasmids...McCormick lab generated knock-out and knock-in mutants, determined protein expression levels from different...critical for reducing Cre toxicity in long-term experiments.  Figure 4: The C-terminus of Cre (CreC...

  19. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...reactions on tissues can be cumbersome Tyramide reagents can be expensive Can only stain with one HRP-RANbody...consider leaving a link to its sequence in the comments below. References Sanes, J.R., & Yamagata, M....versatile, small, sensitive immunohistochemical reagents. PNAS. PubMed PMID: 29440485. Additional Resources...

  20. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    ...about it. Building a diverse team with different talents and expertise Fortunately, the eLife ambassadors... had previous experience organizing open access events and grassroots efforts. After thoughtful conversations...Twitter (@repro4everyone), or email the team. Acknowledgements One of the highlights of 2018 for me was to...
Showing: 741 - 760 of 1020 results