We narrowed to 957 results for: OMP;

... production of viruses used to deliver CRISPR components to cells or organisms under study. In the past...lentivirus production frustrating - a researcher can complete an entire lentiviral production protocol only ...

...field. Of course they are competing with each other, but it's a healthy competition. What an honor for Addgene...

...resulting in a Cas9 with improved specificity. They compared on-site and off-site activity of Sniper-Cas9 to...et al. "Evolved Cas9 variants with broad PAM compatibility and high DNA specificity." Nature 556.7699 (...

...and learn from them. I started at my previous company by taking on responsibilities such as mentoring...facilitated a team meeting to highlight their complementary strengths. By encouraging alignment on goals...

...binding of a new inactive heterotrimeric G protein complex and subsequently another round of signaling.    ...gamma subunits of the heterotrimeric G protein complex that can be used to detect G protein activation...

... creates a non-functional 𝛽-galactosidase. Complementing this mutation by expressing this deleted portion...get started, you will first need a primer that complements your plasmid sequence. Start with a backbone-...

...shRNAs are short RNA sequences which target complementary mRNA sequence for degradation. Viral introduction... virus to which immunity is desired. Instead, components of the virus are now delivered in other forms...

... range from a defined pathway or reaction to a complex event such as cancer or even aging. For both a ...CRISPR, drug, etc.), just with an added baseline component. Alternative screening techniques While CRISPR...

...important in experiments in which viability is being compromised by introduction of foreign RNAs or immunity itself... CRISPR 101: Ribonucleoprotein (RNP) Delivery Components of CRISPR/Cas9 ...

...SSAP system reports an efficiency of up to 20%, comparable to commonly used CRISPR/Cas9 methods for large...error rates overall ranged from 0.3% to 10%, in comparison to the 62-90% error rate seen with Cas9 editors...

... and make a checkmark next to each step as you complete it. Yes, this can be incredibly tedious, but it...several steps into one. Soon you’ll figure out what prompts you need to consistently and accurately run a protocol...

...portion of different antibody isotypes. Binding to complement system proteins, which can initiate a cascade...antibodies through cells and into different body compartments, such as into mucus, tears, or milk. When using...

...increase in the number of endogenous indel edits when compared to unsorted cells (Wen et al., 2018). Thus SRIRACCHA...expression. As a proof of concept the Denham lab compared editing at three different genomic sites in the...

...lentiviruses expressing various gRNAs. It’s better to compare the phenotypic effects of expressing various gRNAs...expression between experiments and over time (as compared to separate transductions with a single-vector...

...protect themselves from foreign DNA. The other component of these systems, methyltransferases, methylate...add methyl groups to specific DNA sequences and companion endonucleases that recognizes and cleave the same...

... Provide perspective on core values at another company/department Give more candid feedback and be able...If they say no, don’t worry about it. It is a compliment to ask someone to share their wisdom and they...

... Addgene move from completing 2-3 large projects a year, on average, to completing 8-10 projects per year...

...Addgene!  Addgene was established as an e-commerce company, which helped it stay ahead of the curve as access...combatting the virus. Our COVID-19 collection, comprised of 1302 plasmids, was shared with researchers ...

...interest is often one of the most challenging components of any immunoimaging assay. Secondary antibodies... (And while I have it on good authority that a complete Western blot can be run in a single day, I really...

...used along other thiol-containing acceptors in competition assays. “I believe that improved biosensors or...related to addiction and pain. Once these are completed, Hubbard will image these neurons using GCaMP7...