We narrowed to 941 results for: OMP;
-
TypeBlog Post...interest is often one of the most challenging components of any immunoimaging assay. Secondary antibodies... (And while I have it on good authority that a complete Western blot can be run in a single day, I really...
-
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog Post...used along other thiol-containing acceptors in competition assays. “I believe that improved biosensors or...related to addiction and pain. Once these are completed, Hubbard will image these neurons using GCaMP7... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog Post... but not necessarily at the same subcellular compartment as the opsin or chemogenetic receptor. This may...Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope... -
Transferable Skills Guide: Cross-team Communication
TypeBlog Post... Addgene move from completing 2-3 large projects a year, on average, to completing 8-10 projects per year... -
Giving gRNAs a Facelift - Synthetic and Beyond
TypeBlog Post...important in experiments in which viability is being compromised by introduction of foreign RNAs or immunity itself... CRISPR 101: Ribonucleoprotein (RNP) Delivery Components of CRISPR/Cas9 ... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog Post... found suitable pairs, they constructed three components: GBPa-VP16 (activation domain); GBPb-GAL4 (DNA-binding..., GFP linked the two GBPs together to create a complete transcription factor, activating luciferase transcription... -
The Future of Research Symposium Boston 2015
TypeBlog Post... about how the system operates and functions” “Complete lack of information on number of postdocs” “Postdocs...Harvard’s Therapeutics Graduate Program has a compulsory internship to be taken in an industry, regulatory... -
Design Tips for Prime Editing
TypeBlog Post...extended to include a primer binding site (PBS) complementary to the genome and a reverse transcriptase (RT...Nureki, O. (2014). Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA. Cell, 156(5), 935... -
Hot Plasmids February 2024
TypeBlog Post...variant has similar photostability and brightness compared to the original, though its maturation is even...cell-specific tropism, with slightly reduced efficacy compared to AA9-X1.1. Importantly, AAV1-X1 is not neutralized... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog Post... Figure 1: SARS-CoV-2 proteins and other components. Image from Maya Kostman for the IGI. ...-LAMP) and a colorimetric readout that can be completed in just 45 minutes. The team deposited the reverse... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog Post...development, recognition and migration, the immune compromise observed in CDG patients is not surprising. However... into functional multi-subunit macromolecular complexes, but a serendipitous discovery led them to investigate... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog Post.... The donor and acceptor fluorophores must be compatible, such that the emission spectrum of the donor...or be detected even if the two proteins form a complex. To help troubleshoot your experimental setup ... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post...multiplexed analysis of cells pre-treated with a compound of interest. Lentiviral vectors Popular Lentiviral...modular and flexible system can be used to construct complex lentiviruses for modification of mammalian cells... -
Hot Plasmids - October 2020
TypeBlog Post...expanding our ability to study and understand the complex interworking of the 86 billion neurons in the human...Sciences. Like many split FPs, split-wrmScarlet is composed of two FP fragments that are not fluorescent on... -
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux
TypeBlog Post... for us all. For example, implementation and compliance for the FLSA changes, including finding money...postdocs nationwide. On this level, FoR is currently compiling a database of institutional responses to the FLSA... -
The Materials Science of Optogenetics Experiments
TypeBlog Post...required to produce your fiber optic implant. Companies such as Thor Labs and Precision Fiber Products...writing. Derek is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org or follow... -
Common Injection Routes in Mice
TypeBlog Post... but they are not recommended for tumorigenic compounds, as circulation is slower, and more institutional...depending on researcher preferences. It sounds complicated but has a pretty low learning curve! Restraint... -
Viral Vectors 101: Producing Your rAAV
TypeBlog Post...or producer cells for further processing. What component(s) you need to collect will depend on the serotype...Absolute determination of single-stranded and self-complementary adeno-associated viral vector genome titers ... -
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog Post...functional utility, iPSCs bypass the transplantation compatibility issues and ethical concerns of ESCs. As iPSCs...in science communication and publishing after completing her postdoctoral training from Massachusetts ... -
The Scientific Conference Poster Session: Tips for Success
TypeBlog Post...practice while seated or with your poster on your computer screen than it is to pretend you’re standing at...guilty of this, and have many times answered a completely different question than the intended one. Take...