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We narrowed to 1,010 results for: PLE

Showing: 741 - 760 of 1010 results

  1. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    ...affect it. While tumors may be the stars, there's plenty of action outside of tumor cells, and recent studies...deeply connected to these 3 stages and contains multiple cell types: fibroblasts, myofibroblasts, neuroendocrine...spread and drug resistance? Although we’ve seen how multiple types of cells affect tumor progression, enzymes...

  2. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...ever like to write about a recent plasmid deposit please sign up here.        AAV delivery of split base... Type IIS restriction sites to easily assemble multiple DNA fragments. This system has been used to construct...controlled by Cre recombination. Each cell can have multiple copies of the transgene that recombine independently...

  3. What's New in CRISPR - November 2021

    Type
    Blog Post
    ... further demonstrated that PRIME-del can be “multiplexed” using pooled paired pegRNAs.  Read the paper...Xue’s lab also uses paired pegRNAs targeting complementary DNA strands to program large genomic deletions... without small insertions. But, instead, PEDAR couples this deletion process to a prime editing enzyme...

  4. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ... the LoxP sites, which is more difficult than a simple knock out. Looking for some permanent knock out...transcripts. When transiently introduced, they can deplete endogenous protein levels for 1-3 days. There’... tunable control These systems can be easily implemented for exogenously introduced proteins, as many ...

  5. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    ...below) Because off-target sequences have less complementarity to the gRNA, they have a higher propensity ...for their on-target and off-target activity at multiple genomic locations in HEK cells. Further testing... Hiroshi, et al. "Crystal structure of Cas9 in complex with guide RNA and target DNA." Cell 156.5 (2014...

  6. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...erlotinib. Looking at barcode abundances among multiple replicate populations, they found that similar...went on to show that combination treatment with multiple drugs reveals even smaller preexisting populations...dynamics in response to cancer therapy using high-complexity barcoding." Nature medicine 21.5 (2015): 440-...

  7. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    ...poster should catch the audience’s eye and draw people in. That can be accomplished with an exciting title...clearly identified and interpreted in less than a couple of minutes. How does one accomplish all of that...column left-to-right, which is the natural way that people expect to read. The key to the success of this ...

  8. A Look at Addgene's QC Process

    Type
    Blog Post
    ...have all heard. It’s a truth that extends to both people and the tools we use. Scientists utilize an array... to stop functioning properly or be something completely different than what was originally intended. ...Addgene receives deposited plasmids, they undergo complete plasmid sequencing using an in-house Illumina ...

  9. CRISPR in the Clinic

    Type
    Blog Post
    ...animal models of disease and cultured cells. For example, in 2013, Jinsong Li and colleagues chose to explore...current delivery technologies are suited for. For example, lipid nanoparticle delivery tends to be trafficked... Multiple Authors...

  10. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...viruses that can infect people, cells, and animals. Some of these viruses are completely inert, but others ...protection are key to lab safety and the same principles apply to viral handling. Gloves are always required...

  11. Hot Plasmids February 2024

    Type
    Blog Post
    ...achieved with a 30-minute incubation, even with multiplexed guides. Figure 1: A) Cell-penetrating ...generated a series of StayGold mutants and identified a triple mutant, Y187F, R144I and T155Q, which they named... Multiple Authors...

  12. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll find in this ... well-characterized Cas enzymes, which rely on multiple active sites. In addition, Casɸ has a minimal ...GFAP.SF-iGluSnFR.A184S-AAV1 here New Dual and Triple feature INTRSECT viral preps from the Deisseroth...

  13. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...others are studying and gives you a sense of what people are excited about. Following scientists in fields...more about authors by exploring author pages (for example, Melina Fan’s author page). These pages show all... Key features Setting up a personalized feed is simple. You can enter as many search terms as you like...

  14. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...anything. Viruses are complicated and they perform complex biological tasks, both of which are factors in ...Ugai et al., 2002). If the virus will be used in a couple of days, some researchers prefer to store freshly...: 16510845. 4. Krajden, Mel, et al. "Effect of multiple freeze-thaw cycles on hepatitis B virus DNA and...

  15. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...loxP, black arrowheads) and one mutated pair (for example- lox511, white arrowheads). It is necessary to ...express GeneR only in the presence of Cre. In this example, Cre would first invert the reporter and GeneR ...2008. A FLEX switch targets Channelrhodopsin-2 to multiple cell types for imaging and long-range circuit ...

  16. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...modification is chromatin remodeling. Chromatin is a complex of DNA and histone proteins that compacts cellular...different disease phenotypes, are two fascinating examples of epigenetics in the form of imprinting. In imprinting...have huge impacts in the way we study and treat complex or multifactorial diseases, enabling faster diagnosis...

  17. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...with knockouts of specific genes. Many years ago, people in the laboratory became frustrated with using ...procedure (illustrated in the YouTube video below) is simple: After obtaining antibiotic-resistant colonies,...initially, or using secondary biological screens. For example, for cancer researchers, if growth properties in...

  18. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    ...Compared to humans and mice, flies have a relatively simple genome. They have four chromosomes: the X (and ...chromosomes essentially are messed up chromosomes with multiple inversions. They are unable to participate in ... dominant phenotypes visible on the flies. For example, second chromosome balancers often have the curly...

  19. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...RNA (pegRNA). The pegRNA consists of a target-complementary spacer, a scaffold sequence, and an extension...screens with oligo libraries where pegRNAs are coupled to their target allowing us to edit and evaluate...efficiencies for diverse prime editing systems in multiple cell types. Cell, 186, 1–17. https://doi.org/10.1016...

  20. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...AdV-mediated transduction of gRNA:Cas9 ribonucleoprotein complexes into transformed and non-transformed cells yielded...a potential platform that could be realized via simple switching of the relevant gRNAs and donor DNA Adenoviral...vector delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array...
Showing: 741 - 760 of 1010 results