We narrowed to 1,006 results for: RING
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TypeBlog Post...spread. They can be inherited by daughter cells during cellular reproduction, similar to the host’s chromosome...metabolic pathways. Fertility (F) plasmids: microbial sharing F plasmids contain the genes for bacteria to form...
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Antibodies 101: Choosing the Right Isotype
TypeBlog Post...options are available to you, it may be worth considering an antibody raised in a different species. ... strategically plan primary/secondary antibody pairing such that each target has a unique species or isotype... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog Post...features that allow such “chemical rescue” in vivo. Engineering the human disease alleles into cells for testing...might consider prescribing GdnHCl to patients suffering from rare diseases arising from Arg mutations.... -
Rabies and Neuronal Tracing
TypeBlog Post...specific locations and give rise to progenitors during growth and development or that blood flows through.... While the EnvA genetic sequences are supplied during production of EnvA-pseudotyped RABV, these sequences... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog Post...CRISPR is a simple and versatile tool for genome engineering, but its utility is dependent on its ability ...Nanoblades editing efficiencies of primary cells. Comparing Nanoblades to Existing CRISPR Delivery Methods... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog Post...resulted altogether in 40 new ABE8 variants. When comparing on-target DNA editing efficiency in a human cell...and ABE7.10. They quantified genomic editing by measuring the reduction in protein expression of these six... -
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog Post...hold great promise for improving or potentially curing human diseases that were previously thought to ...as gene delivery tools: Unlike most naturally occurring viruses used in clinical trials, wild type AAV... -
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux
TypeBlog Post... of Research (FoR) symposia held across the USA during this time have revealed that young scientists are...Biochemistry and Molecular Biology/ FoR Hack Day during which several projects were initiated. Recent developments... -
Plasmids 101: FLEx Vectors
TypeBlog Post...and Ghyselinck NB. A directional strategy for monitoring Cre-mediated recombination at the cellular level...Addgene Blog Read about Other Means of Genome Engineering Using FLEx AAV Vectors? Learn about Viral Vector... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog Post...change (facilitated by the flexible linker) that brings the fluorescent units of the FRET pair together... and diabetes mortality determined by nutrition during parents' and grandparents' slow growth period."... -
Hot Plasmids: Winter 2025
TypeBlog Post...to dicot species. In addition, they generated offspring tomato plants that inherited the genomic edit ...If they do (or if they don't!), please consider sharing your experience with other researchers by contributing... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog Post...the Challenges of Lentivirus Production Tips for Titering Your Lentivirus Preps More resources on Addgene.org...recombinant proteins and viral vectors. Frontiers in Bioengineering and Biotechnology, 9. https://doi.org/10.3389... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog Post...downstream flanking sequences to their input string (Figure 2). This string can then be used as input for the prediction... -
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog Post... use in the protocol, which cover 3 naturally-occurring nanobody structures (concave, loop, and convex...translated protein (sybody), ribosome, and mRNA. During the screening process, displayed sybodies that ... -
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog Post...supernumerary preimplantation embryos that are produced during in vitro fertilization treatments and donated afterwards...produced Ethical concern High. Embryos are destroyed during ESC isolation. Low Chances of post-transplantation... -
3D Printing Meets CRISPR Cas9
TypeBlog Post...their mental model of the protein. At some point during this interrogation process, they will almost invariably...recognize a PAM sequence, allow the complementary base pairing between the guide RNA and the target DNA strand... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog Post...and protein turnover rates. Additionally, by engineering FP fusions associated with cellular organelles...Cas Plasmids - Protein Tagging. Beyond simply monitoring protein localization and activity, CRISPR tagging... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog Post...mouse knockout lines should increase. New genome engineering tools also offer hope for the creation of new...knockout animals or cell lines, please consider sharing them by depositing with Addgene! Figures were ... -
PCR Applications for Copy Number Variation Assays
TypeBlog Post...expression baseline. Copy number is then determined by comparing results to a standard curve. For CNV assays, it...gives an absolute value of DNA concentration. By comparing the number of positive droplets for the gene of... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog Post...FPs that dimerize can form dynamic contacts that bring apposing membranes together and can wrap the membranes...reporter that undergoes a conformational change to bring two FPs on a single protein closer together or further...